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Herpes simplex virus: isolation, cytopathological characterization and antiviral sensitivity()

BACKGROUND: Herpes simplex virus (HSV) infection is an endemic disease and it is estimated that 6095% of the adult population are infected with symptoms that are usually self-limiting, though they can be serious, extensive and prolonged in immunocompromised individuals, highlighted by the emergence...

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Autores principales: Nozawa, Carlos, Hattori, Lilian Yumi, Galhardi, Ligia Carla Faccin, Lopes, Nayara, Bomfim, Wesley Andrade, de Cândido, Ligyana Korki, de Azevedo, Elbens Marcos Minoreli, Gon, Airton dos Santos, Linhares, Rosa Elisa Carvalho
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Dermatologia 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4056703/
https://www.ncbi.nlm.nih.gov/pubmed/24937819
http://dx.doi.org/10.1590/abd1806-4841.20142574
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author Nozawa, Carlos
Hattori, Lilian Yumi
Galhardi, Ligia Carla Faccin
Lopes, Nayara
Bomfim, Wesley Andrade
de Cândido, Ligyana Korki
de Azevedo, Elbens Marcos Minoreli
Gon, Airton dos Santos
Linhares, Rosa Elisa Carvalho
author_facet Nozawa, Carlos
Hattori, Lilian Yumi
Galhardi, Ligia Carla Faccin
Lopes, Nayara
Bomfim, Wesley Andrade
de Cândido, Ligyana Korki
de Azevedo, Elbens Marcos Minoreli
Gon, Airton dos Santos
Linhares, Rosa Elisa Carvalho
author_sort Nozawa, Carlos
collection PubMed
description BACKGROUND: Herpes simplex virus (HSV) infection is an endemic disease and it is estimated that 6095% of the adult population are infected with symptoms that are usually self-limiting, though they can be serious, extensive and prolonged in immunocompromised individuals, highlighted by the emergence of drug-resistant strains. The study of the wild-type HSV strains based on the cytopathogenic features and its antiviral sensitivity are important in the establishment of an antivirogram for controlling the infection. OBJECTIVE: This study sought to isolate and examine the cytopathological characteristics of circulating strains of the Herpes simplex virus, from clinical specimens and their sensitivity to commercially available antiherpesvirus drugs, acyclovir, phosphonophormic acid and trifluridine. METHODS: Herpes simplex virus isolation, cytopathological features and antiviral sensitivity assays were performed in cell culture by tissue culture infectious dose or plaque forming unit assay. RESULTS: From twenty-two clinical specimens, we isolated and adapted nine strains. Overall, the cytopathic effect was detected 24 h post-infection (p.i.) and the presence of syncytia was remarkable 48 h p.i., observed after cell staining. Out of eight isolates, four developed plaques of varying sizes. All the isolates were sensitive to acyclovir, phosphonophormic and trifluridine, with the percentage of virus inhibition (%VI) ranging from 49.7-100%. CONCLUSIONS: The methodology for HSV isolation and characterization is a straightforward approach, but the drug sensitivity test, regarded as being of great practical importance, needs to be better understood.
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spelling pubmed-40567032014-06-17 Herpes simplex virus: isolation, cytopathological characterization and antiviral sensitivity() Nozawa, Carlos Hattori, Lilian Yumi Galhardi, Ligia Carla Faccin Lopes, Nayara Bomfim, Wesley Andrade de Cândido, Ligyana Korki de Azevedo, Elbens Marcos Minoreli Gon, Airton dos Santos Linhares, Rosa Elisa Carvalho An Bras Dermatol Investigation BACKGROUND: Herpes simplex virus (HSV) infection is an endemic disease and it is estimated that 6095% of the adult population are infected with symptoms that are usually self-limiting, though they can be serious, extensive and prolonged in immunocompromised individuals, highlighted by the emergence of drug-resistant strains. The study of the wild-type HSV strains based on the cytopathogenic features and its antiviral sensitivity are important in the establishment of an antivirogram for controlling the infection. OBJECTIVE: This study sought to isolate and examine the cytopathological characteristics of circulating strains of the Herpes simplex virus, from clinical specimens and their sensitivity to commercially available antiherpesvirus drugs, acyclovir, phosphonophormic acid and trifluridine. METHODS: Herpes simplex virus isolation, cytopathological features and antiviral sensitivity assays were performed in cell culture by tissue culture infectious dose or plaque forming unit assay. RESULTS: From twenty-two clinical specimens, we isolated and adapted nine strains. Overall, the cytopathic effect was detected 24 h post-infection (p.i.) and the presence of syncytia was remarkable 48 h p.i., observed after cell staining. Out of eight isolates, four developed plaques of varying sizes. All the isolates were sensitive to acyclovir, phosphonophormic and trifluridine, with the percentage of virus inhibition (%VI) ranging from 49.7-100%. CONCLUSIONS: The methodology for HSV isolation and characterization is a straightforward approach, but the drug sensitivity test, regarded as being of great practical importance, needs to be better understood. Sociedade Brasileira de Dermatologia 2014 /pmc/articles/PMC4056703/ /pubmed/24937819 http://dx.doi.org/10.1590/abd1806-4841.20142574 Text en http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Investigation
Nozawa, Carlos
Hattori, Lilian Yumi
Galhardi, Ligia Carla Faccin
Lopes, Nayara
Bomfim, Wesley Andrade
de Cândido, Ligyana Korki
de Azevedo, Elbens Marcos Minoreli
Gon, Airton dos Santos
Linhares, Rosa Elisa Carvalho
Herpes simplex virus: isolation, cytopathological characterization and antiviral sensitivity()
title Herpes simplex virus: isolation, cytopathological characterization and antiviral sensitivity()
title_full Herpes simplex virus: isolation, cytopathological characterization and antiviral sensitivity()
title_fullStr Herpes simplex virus: isolation, cytopathological characterization and antiviral sensitivity()
title_full_unstemmed Herpes simplex virus: isolation, cytopathological characterization and antiviral sensitivity()
title_short Herpes simplex virus: isolation, cytopathological characterization and antiviral sensitivity()
title_sort herpes simplex virus: isolation, cytopathological characterization and antiviral sensitivity()
topic Investigation
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4056703/
https://www.ncbi.nlm.nih.gov/pubmed/24937819
http://dx.doi.org/10.1590/abd1806-4841.20142574
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