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Patients with sepsis exhibit increased mitochondrial respiratory capacity in peripheral blood immune cells
INTRODUCTION: In sepsis, mitochondria have been associated with both initial dysfunction and subsequent upregulation (biogenesis). However, the evolvement of mitochondrial function in sepsis over time is largely unknown, and we therefore investigated mitochondrial respiration in peripheral blood imm...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4056763/ https://www.ncbi.nlm.nih.gov/pubmed/23883738 http://dx.doi.org/10.1186/cc12831 |
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author | Sjövall, Fredrik Morota, Saori Persson, Johan Hansson, Magnus J Elmér, Eskil |
author_facet | Sjövall, Fredrik Morota, Saori Persson, Johan Hansson, Magnus J Elmér, Eskil |
author_sort | Sjövall, Fredrik |
collection | PubMed |
description | INTRODUCTION: In sepsis, mitochondria have been associated with both initial dysfunction and subsequent upregulation (biogenesis). However, the evolvement of mitochondrial function in sepsis over time is largely unknown, and we therefore investigated mitochondrial respiration in peripheral blood immune cells (PBICs) in sepsis patients during the first week after admission to the intensive care unit (ICU). METHODS: PBICs from 20 patients with severe sepsis or septic shock were analyzed with high-resolution respirometry 3 times after admission to the ICU (within 48 hours, days 3 to 4 and days 6 to 7). Mitochondrial DNA (mtDNA), cytochrome c (Cyt c), and citrate synthase (CS) were measured as indicators of cellular mitochondrial content. RESULTS: In intact PBICs with endogenous substrates, a gradual increase in cellular respiration reached 173% of controls after 1 week (P = 0.001). In permeabilized cells, respiration using substrates of complex I, II, and IV were significantly increased days 1 to 2, reaching 137%, 130%, and 173% of controls, respectively. In parallel, higher levels of CS activity, mtDNA, and Cyt c content in PBICs (211%, 243%, and 331% of controls for the respective indicators were found at days 6 to 7; P < 0.0001). No differences in respiratory capacities were noted between survivors and nonsurvivors at any of the time points measured. CONCLUSIONS: PBICs from patients with sepsis displayed higher mitochondrial respiratory capacities compared with controls, due to an increased mitochondrial content, as indicated by increased mitochondrial DNA, protein content, and enzyme activity. The results argue against mitochondrial respiratory dysfunction in this type of cells in sepsis. |
format | Online Article Text |
id | pubmed-4056763 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-40567632014-06-16 Patients with sepsis exhibit increased mitochondrial respiratory capacity in peripheral blood immune cells Sjövall, Fredrik Morota, Saori Persson, Johan Hansson, Magnus J Elmér, Eskil Crit Care Research INTRODUCTION: In sepsis, mitochondria have been associated with both initial dysfunction and subsequent upregulation (biogenesis). However, the evolvement of mitochondrial function in sepsis over time is largely unknown, and we therefore investigated mitochondrial respiration in peripheral blood immune cells (PBICs) in sepsis patients during the first week after admission to the intensive care unit (ICU). METHODS: PBICs from 20 patients with severe sepsis or septic shock were analyzed with high-resolution respirometry 3 times after admission to the ICU (within 48 hours, days 3 to 4 and days 6 to 7). Mitochondrial DNA (mtDNA), cytochrome c (Cyt c), and citrate synthase (CS) were measured as indicators of cellular mitochondrial content. RESULTS: In intact PBICs with endogenous substrates, a gradual increase in cellular respiration reached 173% of controls after 1 week (P = 0.001). In permeabilized cells, respiration using substrates of complex I, II, and IV were significantly increased days 1 to 2, reaching 137%, 130%, and 173% of controls, respectively. In parallel, higher levels of CS activity, mtDNA, and Cyt c content in PBICs (211%, 243%, and 331% of controls for the respective indicators were found at days 6 to 7; P < 0.0001). No differences in respiratory capacities were noted between survivors and nonsurvivors at any of the time points measured. CONCLUSIONS: PBICs from patients with sepsis displayed higher mitochondrial respiratory capacities compared with controls, due to an increased mitochondrial content, as indicated by increased mitochondrial DNA, protein content, and enzyme activity. The results argue against mitochondrial respiratory dysfunction in this type of cells in sepsis. BioMed Central 2013 2013-07-24 /pmc/articles/PMC4056763/ /pubmed/23883738 http://dx.doi.org/10.1186/cc12831 Text en Copyright © 2013 Sjövall et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Sjövall, Fredrik Morota, Saori Persson, Johan Hansson, Magnus J Elmér, Eskil Patients with sepsis exhibit increased mitochondrial respiratory capacity in peripheral blood immune cells |
title | Patients with sepsis exhibit increased mitochondrial respiratory capacity in peripheral blood immune cells |
title_full | Patients with sepsis exhibit increased mitochondrial respiratory capacity in peripheral blood immune cells |
title_fullStr | Patients with sepsis exhibit increased mitochondrial respiratory capacity in peripheral blood immune cells |
title_full_unstemmed | Patients with sepsis exhibit increased mitochondrial respiratory capacity in peripheral blood immune cells |
title_short | Patients with sepsis exhibit increased mitochondrial respiratory capacity in peripheral blood immune cells |
title_sort | patients with sepsis exhibit increased mitochondrial respiratory capacity in peripheral blood immune cells |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4056763/ https://www.ncbi.nlm.nih.gov/pubmed/23883738 http://dx.doi.org/10.1186/cc12831 |
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