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Molecular Characterisation, Evolution and Expression of Hypoxia-Inducible Factor in Aurelia sp.1

The maintenance of physiological oxygen homeostasis is mediated by hypoxia-inducible factor (HIF), a key transcriptional factor of the PHD-HIF system in all metazoans. However, the molecular evolutionary origin of this central physiological regulatory system is not well characterized. As the earlies...

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Autores principales: Wang, Guoshan, Yu, Zhigang, Zhen, Yu, Mi, Tiezhu, Shi, Yan, Wang, Jianyan, Wang, Minxiao, Sun, Song
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4057343/
https://www.ncbi.nlm.nih.gov/pubmed/24926666
http://dx.doi.org/10.1371/journal.pone.0100057
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author Wang, Guoshan
Yu, Zhigang
Zhen, Yu
Mi, Tiezhu
Shi, Yan
Wang, Jianyan
Wang, Minxiao
Sun, Song
author_facet Wang, Guoshan
Yu, Zhigang
Zhen, Yu
Mi, Tiezhu
Shi, Yan
Wang, Jianyan
Wang, Minxiao
Sun, Song
author_sort Wang, Guoshan
collection PubMed
description The maintenance of physiological oxygen homeostasis is mediated by hypoxia-inducible factor (HIF), a key transcriptional factor of the PHD-HIF system in all metazoans. However, the molecular evolutionary origin of this central physiological regulatory system is not well characterized. As the earliest eumetazoans, Cnidarians can be served as an interesting model for exploring the HIF system from an evolutionary perspective. We identified the complete cDNA sequence of HIF-1α (ASHIF) from the Aurelia sp.1, and the predicted HIF-1α protein (pASHIF) was comprised of 674 amino acids originating from 2,025 bp nucleotides. A Pairwise comparison revealed that pASHIF not only possessed conserved basic helix-loop-helix (bHLH) and Per-Arnt-Sim (PAS) domains but also contained the oxygen dependent degradation (ODD) and the C-terminal transactivation domains (C-TAD), the key domains for hypoxia regulation. As indicated by sequence analysis, the ASHIF gene contains 8 exons interrupted by 7 introns. Western blot analysis indicated that pASHIF that existed in the polyps and medusa of Aurelia. sp.1 was more stable for a hypoxic response than normoxia.
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spelling pubmed-40573432014-06-18 Molecular Characterisation, Evolution and Expression of Hypoxia-Inducible Factor in Aurelia sp.1 Wang, Guoshan Yu, Zhigang Zhen, Yu Mi, Tiezhu Shi, Yan Wang, Jianyan Wang, Minxiao Sun, Song PLoS One Research Article The maintenance of physiological oxygen homeostasis is mediated by hypoxia-inducible factor (HIF), a key transcriptional factor of the PHD-HIF system in all metazoans. However, the molecular evolutionary origin of this central physiological regulatory system is not well characterized. As the earliest eumetazoans, Cnidarians can be served as an interesting model for exploring the HIF system from an evolutionary perspective. We identified the complete cDNA sequence of HIF-1α (ASHIF) from the Aurelia sp.1, and the predicted HIF-1α protein (pASHIF) was comprised of 674 amino acids originating from 2,025 bp nucleotides. A Pairwise comparison revealed that pASHIF not only possessed conserved basic helix-loop-helix (bHLH) and Per-Arnt-Sim (PAS) domains but also contained the oxygen dependent degradation (ODD) and the C-terminal transactivation domains (C-TAD), the key domains for hypoxia regulation. As indicated by sequence analysis, the ASHIF gene contains 8 exons interrupted by 7 introns. Western blot analysis indicated that pASHIF that existed in the polyps and medusa of Aurelia. sp.1 was more stable for a hypoxic response than normoxia. Public Library of Science 2014-06-13 /pmc/articles/PMC4057343/ /pubmed/24926666 http://dx.doi.org/10.1371/journal.pone.0100057 Text en © 2014 Wang et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Wang, Guoshan
Yu, Zhigang
Zhen, Yu
Mi, Tiezhu
Shi, Yan
Wang, Jianyan
Wang, Minxiao
Sun, Song
Molecular Characterisation, Evolution and Expression of Hypoxia-Inducible Factor in Aurelia sp.1
title Molecular Characterisation, Evolution and Expression of Hypoxia-Inducible Factor in Aurelia sp.1
title_full Molecular Characterisation, Evolution and Expression of Hypoxia-Inducible Factor in Aurelia sp.1
title_fullStr Molecular Characterisation, Evolution and Expression of Hypoxia-Inducible Factor in Aurelia sp.1
title_full_unstemmed Molecular Characterisation, Evolution and Expression of Hypoxia-Inducible Factor in Aurelia sp.1
title_short Molecular Characterisation, Evolution and Expression of Hypoxia-Inducible Factor in Aurelia sp.1
title_sort molecular characterisation, evolution and expression of hypoxia-inducible factor in aurelia sp.1
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4057343/
https://www.ncbi.nlm.nih.gov/pubmed/24926666
http://dx.doi.org/10.1371/journal.pone.0100057
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