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Legionella dumoffii Utilizes Exogenous Choline for Phosphatidylcholine Synthesis

Phosphatidycholine (PC) is the major membrane-forming phospholipid in eukaryotes but it has been found in only a limited number of prokaryotes. Bacteria synthesize PC via the phospholipid N-methylation pathway (Pmt) or via the phosphatidylcholine synthase pathway (Pcs) or both. Here, we demonstrated...

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Autores principales: Palusinska-Szysz, Marta, Szuster-Ciesielska, Agnieszka, Kania, Magdalena, Janczarek, Monika, Chmiel, Elżbieta, Danikiewicz, Witold
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4057730/
https://www.ncbi.nlm.nih.gov/pubmed/24821544
http://dx.doi.org/10.3390/ijms15058256
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author Palusinska-Szysz, Marta
Szuster-Ciesielska, Agnieszka
Kania, Magdalena
Janczarek, Monika
Chmiel, Elżbieta
Danikiewicz, Witold
author_facet Palusinska-Szysz, Marta
Szuster-Ciesielska, Agnieszka
Kania, Magdalena
Janczarek, Monika
Chmiel, Elżbieta
Danikiewicz, Witold
author_sort Palusinska-Szysz, Marta
collection PubMed
description Phosphatidycholine (PC) is the major membrane-forming phospholipid in eukaryotes but it has been found in only a limited number of prokaryotes. Bacteria synthesize PC via the phospholipid N-methylation pathway (Pmt) or via the phosphatidylcholine synthase pathway (Pcs) or both. Here, we demonstrated that Legionella dumoffii has the ability to utilize exogenous choline for phosphatidylcholine (PC) synthesis when bacteria grow in the presence of choline. The Pcs seems to be a primary pathway for synthesis of this phospholipid in L. dumoffii. Structurally different PC species were distributed in the outer and inner membranes. As shown by the LC/ESI-MS analyses, PC15:0/15:0, PC16:0/15:0, and PC17:0/17:1 were identified in the outer membrane and PC14:0/16:0, PC16:0/17:1, and PC20:0/15:0 in the inner membrane. L. dumoffii pcsA gene encoding phosphatidylcholine synthase revealed the highest sequence identity to pcsA of L. bozemanae (82%) and L. longbeachae (81%) and lower identity to pcsA of L. drancourtii (78%) and L. pneumophila (71%). The level of TNF-α in THP1-differentiated cells induced by live and temperature-killed L. dumoffii cultured on a medium supplemented with choline was assessed. Live L. dumoffii bacteria cultured on the choline-supplemented medium induced TNF-α three-fold less efficiently than cells grown on the non-supplemented medium. There is an evident effect of PC modification, which impairs the macrophage inflammatory response.
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spelling pubmed-40577302014-06-16 Legionella dumoffii Utilizes Exogenous Choline for Phosphatidylcholine Synthesis Palusinska-Szysz, Marta Szuster-Ciesielska, Agnieszka Kania, Magdalena Janczarek, Monika Chmiel, Elżbieta Danikiewicz, Witold Int J Mol Sci Article Phosphatidycholine (PC) is the major membrane-forming phospholipid in eukaryotes but it has been found in only a limited number of prokaryotes. Bacteria synthesize PC via the phospholipid N-methylation pathway (Pmt) or via the phosphatidylcholine synthase pathway (Pcs) or both. Here, we demonstrated that Legionella dumoffii has the ability to utilize exogenous choline for phosphatidylcholine (PC) synthesis when bacteria grow in the presence of choline. The Pcs seems to be a primary pathway for synthesis of this phospholipid in L. dumoffii. Structurally different PC species were distributed in the outer and inner membranes. As shown by the LC/ESI-MS analyses, PC15:0/15:0, PC16:0/15:0, and PC17:0/17:1 were identified in the outer membrane and PC14:0/16:0, PC16:0/17:1, and PC20:0/15:0 in the inner membrane. L. dumoffii pcsA gene encoding phosphatidylcholine synthase revealed the highest sequence identity to pcsA of L. bozemanae (82%) and L. longbeachae (81%) and lower identity to pcsA of L. drancourtii (78%) and L. pneumophila (71%). The level of TNF-α in THP1-differentiated cells induced by live and temperature-killed L. dumoffii cultured on a medium supplemented with choline was assessed. Live L. dumoffii bacteria cultured on the choline-supplemented medium induced TNF-α three-fold less efficiently than cells grown on the non-supplemented medium. There is an evident effect of PC modification, which impairs the macrophage inflammatory response. Molecular Diversity Preservation International (MDPI) 2014-05-09 /pmc/articles/PMC4057730/ /pubmed/24821544 http://dx.doi.org/10.3390/ijms15058256 Text en © 2014 by the authors; licensee MDPI, Basel, Switzerland http://creativecommons.org/licenses/by/3.0/ This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Palusinska-Szysz, Marta
Szuster-Ciesielska, Agnieszka
Kania, Magdalena
Janczarek, Monika
Chmiel, Elżbieta
Danikiewicz, Witold
Legionella dumoffii Utilizes Exogenous Choline for Phosphatidylcholine Synthesis
title Legionella dumoffii Utilizes Exogenous Choline for Phosphatidylcholine Synthesis
title_full Legionella dumoffii Utilizes Exogenous Choline for Phosphatidylcholine Synthesis
title_fullStr Legionella dumoffii Utilizes Exogenous Choline for Phosphatidylcholine Synthesis
title_full_unstemmed Legionella dumoffii Utilizes Exogenous Choline for Phosphatidylcholine Synthesis
title_short Legionella dumoffii Utilizes Exogenous Choline for Phosphatidylcholine Synthesis
title_sort legionella dumoffii utilizes exogenous choline for phosphatidylcholine synthesis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4057730/
https://www.ncbi.nlm.nih.gov/pubmed/24821544
http://dx.doi.org/10.3390/ijms15058256
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