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MicroRNA-103 Promotes Colorectal Cancer by Targeting Tumor Suppressor DICER and PTEN

MicroRNAs (miRNAs) are a class of small, noncoding RNAs that act as key regulators in various physiological and pathological processes. However, the regulatory mechanisms for miRNAs in colorectal cancer remain largely unknown. Here, we found that miR-103 is up-regulated in colorectal cancer and its...

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Detalles Bibliográficos
Autores principales: Geng, Li, Sun, Bing, Gao, Bo, Wang, Zheng, Quan, Cheng, Wei, Feng, Fang, Xue-Dong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4057742/
https://www.ncbi.nlm.nih.gov/pubmed/24828205
http://dx.doi.org/10.3390/ijms15058458
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author Geng, Li
Sun, Bing
Gao, Bo
Wang, Zheng
Quan, Cheng
Wei, Feng
Fang, Xue-Dong
author_facet Geng, Li
Sun, Bing
Gao, Bo
Wang, Zheng
Quan, Cheng
Wei, Feng
Fang, Xue-Dong
author_sort Geng, Li
collection PubMed
description MicroRNAs (miRNAs) are a class of small, noncoding RNAs that act as key regulators in various physiological and pathological processes. However, the regulatory mechanisms for miRNAs in colorectal cancer remain largely unknown. Here, we found that miR-103 is up-regulated in colorectal cancer and its overexpression is closely associated with tumor proliferation and migration. In addition, repressing the expression of miR-103 apparently inhibits colorectal cancer cell proliferation and migration in vitro and HCT-116 xenograft tumor growth in vivo. Subsequent software analysis and dual-luciferase reporter assay identified two tumor suppressor genes DICER and PTEN as direct targets of miR-103, and up-regulation of DICER and PTEN obtained similar results to that occurred in the silencing of miR-103. In addition, restoration of DICER and PTEN can inhibit miR-103-induced colorectal cancer cell proliferation and migration. Our data collectively demonstrate that miR-103 is an oncogene miRNA that promotes colorectal cancer proliferation and migration through down-regulation of the tumor suppressor genes DICER and PTEN. Thus, miR-103 may represent a new potential diagnostic and therapeutic target for colorectal cancer treatment.
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spelling pubmed-40577422014-06-16 MicroRNA-103 Promotes Colorectal Cancer by Targeting Tumor Suppressor DICER and PTEN Geng, Li Sun, Bing Gao, Bo Wang, Zheng Quan, Cheng Wei, Feng Fang, Xue-Dong Int J Mol Sci Article MicroRNAs (miRNAs) are a class of small, noncoding RNAs that act as key regulators in various physiological and pathological processes. However, the regulatory mechanisms for miRNAs in colorectal cancer remain largely unknown. Here, we found that miR-103 is up-regulated in colorectal cancer and its overexpression is closely associated with tumor proliferation and migration. In addition, repressing the expression of miR-103 apparently inhibits colorectal cancer cell proliferation and migration in vitro and HCT-116 xenograft tumor growth in vivo. Subsequent software analysis and dual-luciferase reporter assay identified two tumor suppressor genes DICER and PTEN as direct targets of miR-103, and up-regulation of DICER and PTEN obtained similar results to that occurred in the silencing of miR-103. In addition, restoration of DICER and PTEN can inhibit miR-103-induced colorectal cancer cell proliferation and migration. Our data collectively demonstrate that miR-103 is an oncogene miRNA that promotes colorectal cancer proliferation and migration through down-regulation of the tumor suppressor genes DICER and PTEN. Thus, miR-103 may represent a new potential diagnostic and therapeutic target for colorectal cancer treatment. Molecular Diversity Preservation International (MDPI) 2014-05-13 /pmc/articles/PMC4057742/ /pubmed/24828205 http://dx.doi.org/10.3390/ijms15058458 Text en © 2014 by the authors; licensee MDPI, Basel, Switzerland http://creativecommons.org/licenses/by/3.0/ This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Geng, Li
Sun, Bing
Gao, Bo
Wang, Zheng
Quan, Cheng
Wei, Feng
Fang, Xue-Dong
MicroRNA-103 Promotes Colorectal Cancer by Targeting Tumor Suppressor DICER and PTEN
title MicroRNA-103 Promotes Colorectal Cancer by Targeting Tumor Suppressor DICER and PTEN
title_full MicroRNA-103 Promotes Colorectal Cancer by Targeting Tumor Suppressor DICER and PTEN
title_fullStr MicroRNA-103 Promotes Colorectal Cancer by Targeting Tumor Suppressor DICER and PTEN
title_full_unstemmed MicroRNA-103 Promotes Colorectal Cancer by Targeting Tumor Suppressor DICER and PTEN
title_short MicroRNA-103 Promotes Colorectal Cancer by Targeting Tumor Suppressor DICER and PTEN
title_sort microrna-103 promotes colorectal cancer by targeting tumor suppressor dicer and pten
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4057742/
https://www.ncbi.nlm.nih.gov/pubmed/24828205
http://dx.doi.org/10.3390/ijms15058458
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