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Stimulation of Bone Healing by Sustained Bone Morphogenetic Protein 2 (BMP-2) Delivery

The aim of the study was to investigate the effect of a sustained release of bone morphogenetic protein2 (BMP-2) incorporated in a polymeric implant coating on bone healing. In vitro analysis revealed a sustained, but incomplete BMP-2 release until Day 42. For the in vivo study, the rat tibia osteot...

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Autores principales: Faßbender, Mirja, Minkwitz, Susann, Strobel, Catrin, Schmidmaier, Gerhard, Wildemann, Britt
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4057747/
https://www.ncbi.nlm.nih.gov/pubmed/24830556
http://dx.doi.org/10.3390/ijms15058539
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author Faßbender, Mirja
Minkwitz, Susann
Strobel, Catrin
Schmidmaier, Gerhard
Wildemann, Britt
author_facet Faßbender, Mirja
Minkwitz, Susann
Strobel, Catrin
Schmidmaier, Gerhard
Wildemann, Britt
author_sort Faßbender, Mirja
collection PubMed
description The aim of the study was to investigate the effect of a sustained release of bone morphogenetic protein2 (BMP-2) incorporated in a polymeric implant coating on bone healing. In vitro analysis revealed a sustained, but incomplete BMP-2 release until Day 42. For the in vivo study, the rat tibia osteotomy was stabilized either with control or BMP-2 coated wires, and the healing progress was followed by micro computed tomography (μCT), biomechanical testing and histology at Days 10, 28, 42 and 84. MicroCT showed an accelerated formation of mineralized callus, as well as remodeling and an increase of mineralized/total callus volume (p = 0.021) at Day 42 in the BMP-2 group compared to the control. Histology revealed an increased callus mineralization at Days 42 and 84 (p = 0.006) with reduced cartilage at Day 84 (p = 0.004) in the BMP-2 group. Biomechanical stiffness was significantly higher in the BMP-2 group (p = 0.045) at Day 42. In summary, bone healing was enhanced after sustained BMP-2 application compared to the control. Using the same drug delivery system, but a burst release of BMP-2, a previous published study showed a similar positive effect on bone healing. Distinct differences in the healing outcome might be explained due to the different BMP release kinetics and dosages. However, further studies are necessary to adapt the optimal release profiles to physiological mechanisms.
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spelling pubmed-40577472014-06-16 Stimulation of Bone Healing by Sustained Bone Morphogenetic Protein 2 (BMP-2) Delivery Faßbender, Mirja Minkwitz, Susann Strobel, Catrin Schmidmaier, Gerhard Wildemann, Britt Int J Mol Sci Article The aim of the study was to investigate the effect of a sustained release of bone morphogenetic protein2 (BMP-2) incorporated in a polymeric implant coating on bone healing. In vitro analysis revealed a sustained, but incomplete BMP-2 release until Day 42. For the in vivo study, the rat tibia osteotomy was stabilized either with control or BMP-2 coated wires, and the healing progress was followed by micro computed tomography (μCT), biomechanical testing and histology at Days 10, 28, 42 and 84. MicroCT showed an accelerated formation of mineralized callus, as well as remodeling and an increase of mineralized/total callus volume (p = 0.021) at Day 42 in the BMP-2 group compared to the control. Histology revealed an increased callus mineralization at Days 42 and 84 (p = 0.006) with reduced cartilage at Day 84 (p = 0.004) in the BMP-2 group. Biomechanical stiffness was significantly higher in the BMP-2 group (p = 0.045) at Day 42. In summary, bone healing was enhanced after sustained BMP-2 application compared to the control. Using the same drug delivery system, but a burst release of BMP-2, a previous published study showed a similar positive effect on bone healing. Distinct differences in the healing outcome might be explained due to the different BMP release kinetics and dosages. However, further studies are necessary to adapt the optimal release profiles to physiological mechanisms. Molecular Diversity Preservation International (MDPI) 2014-05-14 /pmc/articles/PMC4057747/ /pubmed/24830556 http://dx.doi.org/10.3390/ijms15058539 Text en © 2014 by the authors; licensee MDPI, Basel, Switzerland http://creativecommons.org/licenses/by/3.0/ This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Faßbender, Mirja
Minkwitz, Susann
Strobel, Catrin
Schmidmaier, Gerhard
Wildemann, Britt
Stimulation of Bone Healing by Sustained Bone Morphogenetic Protein 2 (BMP-2) Delivery
title Stimulation of Bone Healing by Sustained Bone Morphogenetic Protein 2 (BMP-2) Delivery
title_full Stimulation of Bone Healing by Sustained Bone Morphogenetic Protein 2 (BMP-2) Delivery
title_fullStr Stimulation of Bone Healing by Sustained Bone Morphogenetic Protein 2 (BMP-2) Delivery
title_full_unstemmed Stimulation of Bone Healing by Sustained Bone Morphogenetic Protein 2 (BMP-2) Delivery
title_short Stimulation of Bone Healing by Sustained Bone Morphogenetic Protein 2 (BMP-2) Delivery
title_sort stimulation of bone healing by sustained bone morphogenetic protein 2 (bmp-2) delivery
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4057747/
https://www.ncbi.nlm.nih.gov/pubmed/24830556
http://dx.doi.org/10.3390/ijms15058539
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