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Antibody-Conjugated Paramagnetic Nanobeads: Kinetics of Bead-Cell Binding

Specific labelling of target cell surfaces using antibody-conjugated paramagnetic nanobeads is essential for efficient magnetic cell separation. However, studies examining parameters determining the kinetics of bead-cell binding are scarce. The present study determines the binding rates for specific...

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Detalles Bibliográficos
Autores principales: Waseem, Shahid, Allen, Michael A., Schreier, Stefan, Udomsangpetch, Rachanee, Bhakdi, Sebastian C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4057761/
https://www.ncbi.nlm.nih.gov/pubmed/24852940
http://dx.doi.org/10.3390/ijms15058821
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author Waseem, Shahid
Allen, Michael A.
Schreier, Stefan
Udomsangpetch, Rachanee
Bhakdi, Sebastian C.
author_facet Waseem, Shahid
Allen, Michael A.
Schreier, Stefan
Udomsangpetch, Rachanee
Bhakdi, Sebastian C.
author_sort Waseem, Shahid
collection PubMed
description Specific labelling of target cell surfaces using antibody-conjugated paramagnetic nanobeads is essential for efficient magnetic cell separation. However, studies examining parameters determining the kinetics of bead-cell binding are scarce. The present study determines the binding rates for specific and unspecific binding of 150 nm paramagnetic nanobeads to highly purified target and non-target cells. Beads bound to cells were enumerated spectrophotometrically. Results show that the initial bead-cell binding rate and saturation levels depend on initial bead concentration and fit curves of the form A(1 − exp(−kt)). Unspecific binding within conventional experimental time-spans (up to 60 min) was not detectable photometrically. For CD3-positive cells, the probability of specific binding was found to be around 80 times larger than that of unspecific binding.
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spelling pubmed-40577612014-06-16 Antibody-Conjugated Paramagnetic Nanobeads: Kinetics of Bead-Cell Binding Waseem, Shahid Allen, Michael A. Schreier, Stefan Udomsangpetch, Rachanee Bhakdi, Sebastian C. Int J Mol Sci Article Specific labelling of target cell surfaces using antibody-conjugated paramagnetic nanobeads is essential for efficient magnetic cell separation. However, studies examining parameters determining the kinetics of bead-cell binding are scarce. The present study determines the binding rates for specific and unspecific binding of 150 nm paramagnetic nanobeads to highly purified target and non-target cells. Beads bound to cells were enumerated spectrophotometrically. Results show that the initial bead-cell binding rate and saturation levels depend on initial bead concentration and fit curves of the form A(1 − exp(−kt)). Unspecific binding within conventional experimental time-spans (up to 60 min) was not detectable photometrically. For CD3-positive cells, the probability of specific binding was found to be around 80 times larger than that of unspecific binding. Molecular Diversity Preservation International (MDPI) 2014-05-19 /pmc/articles/PMC4057761/ /pubmed/24852940 http://dx.doi.org/10.3390/ijms15058821 Text en © 2014 by the authors; licensee MDPI, Basel, Switzerland http://creativecommons.org/licenses/by/3.0/ This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Waseem, Shahid
Allen, Michael A.
Schreier, Stefan
Udomsangpetch, Rachanee
Bhakdi, Sebastian C.
Antibody-Conjugated Paramagnetic Nanobeads: Kinetics of Bead-Cell Binding
title Antibody-Conjugated Paramagnetic Nanobeads: Kinetics of Bead-Cell Binding
title_full Antibody-Conjugated Paramagnetic Nanobeads: Kinetics of Bead-Cell Binding
title_fullStr Antibody-Conjugated Paramagnetic Nanobeads: Kinetics of Bead-Cell Binding
title_full_unstemmed Antibody-Conjugated Paramagnetic Nanobeads: Kinetics of Bead-Cell Binding
title_short Antibody-Conjugated Paramagnetic Nanobeads: Kinetics of Bead-Cell Binding
title_sort antibody-conjugated paramagnetic nanobeads: kinetics of bead-cell binding
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4057761/
https://www.ncbi.nlm.nih.gov/pubmed/24852940
http://dx.doi.org/10.3390/ijms15058821
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