Cargando…
Antibody-Conjugated Paramagnetic Nanobeads: Kinetics of Bead-Cell Binding
Specific labelling of target cell surfaces using antibody-conjugated paramagnetic nanobeads is essential for efficient magnetic cell separation. However, studies examining parameters determining the kinetics of bead-cell binding are scarce. The present study determines the binding rates for specific...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Molecular Diversity Preservation International (MDPI)
2014
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4057761/ https://www.ncbi.nlm.nih.gov/pubmed/24852940 http://dx.doi.org/10.3390/ijms15058821 |
_version_ | 1782321027178561536 |
---|---|
author | Waseem, Shahid Allen, Michael A. Schreier, Stefan Udomsangpetch, Rachanee Bhakdi, Sebastian C. |
author_facet | Waseem, Shahid Allen, Michael A. Schreier, Stefan Udomsangpetch, Rachanee Bhakdi, Sebastian C. |
author_sort | Waseem, Shahid |
collection | PubMed |
description | Specific labelling of target cell surfaces using antibody-conjugated paramagnetic nanobeads is essential for efficient magnetic cell separation. However, studies examining parameters determining the kinetics of bead-cell binding are scarce. The present study determines the binding rates for specific and unspecific binding of 150 nm paramagnetic nanobeads to highly purified target and non-target cells. Beads bound to cells were enumerated spectrophotometrically. Results show that the initial bead-cell binding rate and saturation levels depend on initial bead concentration and fit curves of the form A(1 − exp(−kt)). Unspecific binding within conventional experimental time-spans (up to 60 min) was not detectable photometrically. For CD3-positive cells, the probability of specific binding was found to be around 80 times larger than that of unspecific binding. |
format | Online Article Text |
id | pubmed-4057761 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Molecular Diversity Preservation International (MDPI) |
record_format | MEDLINE/PubMed |
spelling | pubmed-40577612014-06-16 Antibody-Conjugated Paramagnetic Nanobeads: Kinetics of Bead-Cell Binding Waseem, Shahid Allen, Michael A. Schreier, Stefan Udomsangpetch, Rachanee Bhakdi, Sebastian C. Int J Mol Sci Article Specific labelling of target cell surfaces using antibody-conjugated paramagnetic nanobeads is essential for efficient magnetic cell separation. However, studies examining parameters determining the kinetics of bead-cell binding are scarce. The present study determines the binding rates for specific and unspecific binding of 150 nm paramagnetic nanobeads to highly purified target and non-target cells. Beads bound to cells were enumerated spectrophotometrically. Results show that the initial bead-cell binding rate and saturation levels depend on initial bead concentration and fit curves of the form A(1 − exp(−kt)). Unspecific binding within conventional experimental time-spans (up to 60 min) was not detectable photometrically. For CD3-positive cells, the probability of specific binding was found to be around 80 times larger than that of unspecific binding. Molecular Diversity Preservation International (MDPI) 2014-05-19 /pmc/articles/PMC4057761/ /pubmed/24852940 http://dx.doi.org/10.3390/ijms15058821 Text en © 2014 by the authors; licensee MDPI, Basel, Switzerland http://creativecommons.org/licenses/by/3.0/ This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
spellingShingle | Article Waseem, Shahid Allen, Michael A. Schreier, Stefan Udomsangpetch, Rachanee Bhakdi, Sebastian C. Antibody-Conjugated Paramagnetic Nanobeads: Kinetics of Bead-Cell Binding |
title | Antibody-Conjugated Paramagnetic Nanobeads: Kinetics of Bead-Cell Binding |
title_full | Antibody-Conjugated Paramagnetic Nanobeads: Kinetics of Bead-Cell Binding |
title_fullStr | Antibody-Conjugated Paramagnetic Nanobeads: Kinetics of Bead-Cell Binding |
title_full_unstemmed | Antibody-Conjugated Paramagnetic Nanobeads: Kinetics of Bead-Cell Binding |
title_short | Antibody-Conjugated Paramagnetic Nanobeads: Kinetics of Bead-Cell Binding |
title_sort | antibody-conjugated paramagnetic nanobeads: kinetics of bead-cell binding |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4057761/ https://www.ncbi.nlm.nih.gov/pubmed/24852940 http://dx.doi.org/10.3390/ijms15058821 |
work_keys_str_mv | AT waseemshahid antibodyconjugatedparamagneticnanobeadskineticsofbeadcellbinding AT allenmichaela antibodyconjugatedparamagneticnanobeadskineticsofbeadcellbinding AT schreierstefan antibodyconjugatedparamagneticnanobeadskineticsofbeadcellbinding AT udomsangpetchrachanee antibodyconjugatedparamagneticnanobeadskineticsofbeadcellbinding AT bhakdisebastianc antibodyconjugatedparamagneticnanobeadskineticsofbeadcellbinding |