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Clinical Comparisons of Two Free Light Chain Assays to Immunofixation Electrophoresis for Detecting Monoclonal Gammopathy

Free light chains (FLCs) are useful biomarkers for the diagnosis and monitoring of various plasma cell dyscrasias. One hundred fifty-seven samples from 120 patients for screening or monitoring of monoclonal gammopathy (MG) were included. The new N Latex FLC assays (Siemens Healthcare Diagnostics Gmb...

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Autores principales: Kim, Han-Sung, Kim, Hyun Soo, Shin, Kyu-Sung, Song, Wonkeun, Kim, Hyo Jung, Kim, Hyeong Su, Park, Min-Jeong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4058286/
https://www.ncbi.nlm.nih.gov/pubmed/24971342
http://dx.doi.org/10.1155/2014/647238
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author Kim, Han-Sung
Kim, Hyun Soo
Shin, Kyu-Sung
Song, Wonkeun
Kim, Hyo Jung
Kim, Hyeong Su
Park, Min-Jeong
author_facet Kim, Han-Sung
Kim, Hyun Soo
Shin, Kyu-Sung
Song, Wonkeun
Kim, Hyo Jung
Kim, Hyeong Su
Park, Min-Jeong
author_sort Kim, Han-Sung
collection PubMed
description Free light chains (FLCs) are useful biomarkers for the diagnosis and monitoring of various plasma cell dyscrasias. One hundred fifty-seven samples from 120 patients for screening or monitoring of monoclonal gammopathy (MG) were included. The new N Latex FLC assays (Siemens Healthcare Diagnostics GmbH, Germany) were compared with the Freelite FLC assays (The Binding Site Ltd., UK) and the results were analyzed with those of immunofixation electrophoresis (IFE). The Freelite FLC assay showed significantly wider assay ranges than the N Latex FLC assay. The correlation coefficients of the two FLC kappa (κ) assays, lambda (λ) assays, and the κ/λ ratio were 0.9792, 0.8264, and 0.9064, respectively. The concordance rate was 84.7% for the FLC κ assays, 79.6% for FLC λ, and 89.2% for the κ/λ ratio. The clinical sensitivity and specificity of the κ/λ ratios were 72.2% and 93.6% for the Freelite assay and 64.6% and 100% for the N Latex FLC assay. Two FLC assays showed good correlations and concordance. However, the clinical sensitivity of the κ/λ ratio was higher in the Freelite FLC assays; clinical specificity was higher in the N Latex FLC assay. Both FLC assays seem to have limited clinical utility in detecting MG in certain clinical settings.
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spelling pubmed-40582862014-06-26 Clinical Comparisons of Two Free Light Chain Assays to Immunofixation Electrophoresis for Detecting Monoclonal Gammopathy Kim, Han-Sung Kim, Hyun Soo Shin, Kyu-Sung Song, Wonkeun Kim, Hyo Jung Kim, Hyeong Su Park, Min-Jeong Biomed Res Int Research Article Free light chains (FLCs) are useful biomarkers for the diagnosis and monitoring of various plasma cell dyscrasias. One hundred fifty-seven samples from 120 patients for screening or monitoring of monoclonal gammopathy (MG) were included. The new N Latex FLC assays (Siemens Healthcare Diagnostics GmbH, Germany) were compared with the Freelite FLC assays (The Binding Site Ltd., UK) and the results were analyzed with those of immunofixation electrophoresis (IFE). The Freelite FLC assay showed significantly wider assay ranges than the N Latex FLC assay. The correlation coefficients of the two FLC kappa (κ) assays, lambda (λ) assays, and the κ/λ ratio were 0.9792, 0.8264, and 0.9064, respectively. The concordance rate was 84.7% for the FLC κ assays, 79.6% for FLC λ, and 89.2% for the κ/λ ratio. The clinical sensitivity and specificity of the κ/λ ratios were 72.2% and 93.6% for the Freelite assay and 64.6% and 100% for the N Latex FLC assay. Two FLC assays showed good correlations and concordance. However, the clinical sensitivity of the κ/λ ratio was higher in the Freelite FLC assays; clinical specificity was higher in the N Latex FLC assay. Both FLC assays seem to have limited clinical utility in detecting MG in certain clinical settings. Hindawi Publishing Corporation 2014 2014-05-27 /pmc/articles/PMC4058286/ /pubmed/24971342 http://dx.doi.org/10.1155/2014/647238 Text en Copyright © 2014 Han-Sung Kim et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Kim, Han-Sung
Kim, Hyun Soo
Shin, Kyu-Sung
Song, Wonkeun
Kim, Hyo Jung
Kim, Hyeong Su
Park, Min-Jeong
Clinical Comparisons of Two Free Light Chain Assays to Immunofixation Electrophoresis for Detecting Monoclonal Gammopathy
title Clinical Comparisons of Two Free Light Chain Assays to Immunofixation Electrophoresis for Detecting Monoclonal Gammopathy
title_full Clinical Comparisons of Two Free Light Chain Assays to Immunofixation Electrophoresis for Detecting Monoclonal Gammopathy
title_fullStr Clinical Comparisons of Two Free Light Chain Assays to Immunofixation Electrophoresis for Detecting Monoclonal Gammopathy
title_full_unstemmed Clinical Comparisons of Two Free Light Chain Assays to Immunofixation Electrophoresis for Detecting Monoclonal Gammopathy
title_short Clinical Comparisons of Two Free Light Chain Assays to Immunofixation Electrophoresis for Detecting Monoclonal Gammopathy
title_sort clinical comparisons of two free light chain assays to immunofixation electrophoresis for detecting monoclonal gammopathy
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4058286/
https://www.ncbi.nlm.nih.gov/pubmed/24971342
http://dx.doi.org/10.1155/2014/647238
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