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Commercial Bovine Proteoglycan Is Highly Arthritogenic and Can Be Used as an Alternative Antigen Source for PGIA Model

Rheumatoid arthritis (RA) is the most common systemic autoimmune disease. It affects mainly the joints, causing synovitis, cartilage destruction, and bone erosion. Many experimental models are used to study the mechanisms involved in immunopathogenesis and new therapies for this disease. Proteoglyca...

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Autores principales: Ishikawa, Larissa Lumi Watanabe, Colavite, Priscila Maria, da Rosa, Larissa Camargo, Balbino, Bianca, França, Thais Graziela Donegá, Zorzella-Pezavento, Sofia Fernanda Gonçalves, Chiuso-Minicucci, Fernanda, Sartori, Alexandrina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4058295/
https://www.ncbi.nlm.nih.gov/pubmed/24971313
http://dx.doi.org/10.1155/2014/148594
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author Ishikawa, Larissa Lumi Watanabe
Colavite, Priscila Maria
da Rosa, Larissa Camargo
Balbino, Bianca
França, Thais Graziela Donegá
Zorzella-Pezavento, Sofia Fernanda Gonçalves
Chiuso-Minicucci, Fernanda
Sartori, Alexandrina
author_facet Ishikawa, Larissa Lumi Watanabe
Colavite, Priscila Maria
da Rosa, Larissa Camargo
Balbino, Bianca
França, Thais Graziela Donegá
Zorzella-Pezavento, Sofia Fernanda Gonçalves
Chiuso-Minicucci, Fernanda
Sartori, Alexandrina
author_sort Ishikawa, Larissa Lumi Watanabe
collection PubMed
description Rheumatoid arthritis (RA) is the most common systemic autoimmune disease. It affects mainly the joints, causing synovitis, cartilage destruction, and bone erosion. Many experimental models are used to study the mechanisms involved in immunopathogenesis and new therapies for this disease. Proteoglycan-induced arthritis (PGIA) is a widely used model based on the cross-reactivity of injected foreign (usually human) PG and mice self-PG. Considering the complexity of the extraction and purification of human PG, in this study we evaluated the arthritogenicity of bovine PG that is commercially available. Bovine PG was highly arthritogenic, triggering 100% incidence of arthritis in female BALB/c retired breeder mice. Animals immunized with bovine PG presented clinical symptoms and histopathological features similar to human RA and other experimental models. Moreover, bovine PG immunization determined higher levels of proinflammatory and anti-inflammatory cytokines in arthritic mice compared to healthy ones. As expected, only the arthritic group produced IgG1 and IgG2a antibodies against PG. Thus, commercial bovine PG can be used as an alternative antigenic source to PGIA for the study of many RA aspects, including the immunopathogenesis of the disease and also the development of new therapies.
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spelling pubmed-40582952014-06-26 Commercial Bovine Proteoglycan Is Highly Arthritogenic and Can Be Used as an Alternative Antigen Source for PGIA Model Ishikawa, Larissa Lumi Watanabe Colavite, Priscila Maria da Rosa, Larissa Camargo Balbino, Bianca França, Thais Graziela Donegá Zorzella-Pezavento, Sofia Fernanda Gonçalves Chiuso-Minicucci, Fernanda Sartori, Alexandrina Biomed Res Int Research Article Rheumatoid arthritis (RA) is the most common systemic autoimmune disease. It affects mainly the joints, causing synovitis, cartilage destruction, and bone erosion. Many experimental models are used to study the mechanisms involved in immunopathogenesis and new therapies for this disease. Proteoglycan-induced arthritis (PGIA) is a widely used model based on the cross-reactivity of injected foreign (usually human) PG and mice self-PG. Considering the complexity of the extraction and purification of human PG, in this study we evaluated the arthritogenicity of bovine PG that is commercially available. Bovine PG was highly arthritogenic, triggering 100% incidence of arthritis in female BALB/c retired breeder mice. Animals immunized with bovine PG presented clinical symptoms and histopathological features similar to human RA and other experimental models. Moreover, bovine PG immunization determined higher levels of proinflammatory and anti-inflammatory cytokines in arthritic mice compared to healthy ones. As expected, only the arthritic group produced IgG1 and IgG2a antibodies against PG. Thus, commercial bovine PG can be used as an alternative antigenic source to PGIA for the study of many RA aspects, including the immunopathogenesis of the disease and also the development of new therapies. Hindawi Publishing Corporation 2014 2014-05-27 /pmc/articles/PMC4058295/ /pubmed/24971313 http://dx.doi.org/10.1155/2014/148594 Text en Copyright © 2014 Larissa Lumi Watanabe Ishikawa et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Ishikawa, Larissa Lumi Watanabe
Colavite, Priscila Maria
da Rosa, Larissa Camargo
Balbino, Bianca
França, Thais Graziela Donegá
Zorzella-Pezavento, Sofia Fernanda Gonçalves
Chiuso-Minicucci, Fernanda
Sartori, Alexandrina
Commercial Bovine Proteoglycan Is Highly Arthritogenic and Can Be Used as an Alternative Antigen Source for PGIA Model
title Commercial Bovine Proteoglycan Is Highly Arthritogenic and Can Be Used as an Alternative Antigen Source for PGIA Model
title_full Commercial Bovine Proteoglycan Is Highly Arthritogenic and Can Be Used as an Alternative Antigen Source for PGIA Model
title_fullStr Commercial Bovine Proteoglycan Is Highly Arthritogenic and Can Be Used as an Alternative Antigen Source for PGIA Model
title_full_unstemmed Commercial Bovine Proteoglycan Is Highly Arthritogenic and Can Be Used as an Alternative Antigen Source for PGIA Model
title_short Commercial Bovine Proteoglycan Is Highly Arthritogenic and Can Be Used as an Alternative Antigen Source for PGIA Model
title_sort commercial bovine proteoglycan is highly arthritogenic and can be used as an alternative antigen source for pgia model
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4058295/
https://www.ncbi.nlm.nih.gov/pubmed/24971313
http://dx.doi.org/10.1155/2014/148594
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