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Development and Maturation of the Immune System in Preterm Neonates: Results from a Whole Genome Expression Study

To expand the knowledge about the consecutive expression of genes involved in the immune system development in preterm neonates and to verify if the environment changes the gene expression after birth we conducted a prospective study that included three cohorts: (A) extremely (gestational age (GA):...

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Autores principales: Zasada, Magdalena, Kwinta, Przemko, Durlak, Wojciech, Bik-Multanowski, Mirosław, Madetko-Talowska, Anna, Pietrzyk, Jacek Józef
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4058491/
https://www.ncbi.nlm.nih.gov/pubmed/24982884
http://dx.doi.org/10.1155/2014/498318
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author Zasada, Magdalena
Kwinta, Przemko
Durlak, Wojciech
Bik-Multanowski, Mirosław
Madetko-Talowska, Anna
Pietrzyk, Jacek Józef
author_facet Zasada, Magdalena
Kwinta, Przemko
Durlak, Wojciech
Bik-Multanowski, Mirosław
Madetko-Talowska, Anna
Pietrzyk, Jacek Józef
author_sort Zasada, Magdalena
collection PubMed
description To expand the knowledge about the consecutive expression of genes involved in the immune system development in preterm neonates and to verify if the environment changes the gene expression after birth we conducted a prospective study that included three cohorts: (A) extremely (gestational age (GA): 23–26 weeks; n = 41), (B) very (GA: 27–29 weeks; n = 39), and (C) moderately preterm infants (GA: 30–32 weeks; n = 33). Blood samples were drawn from the study participants on the 5th and 28th day of life (DOL). The mRNA samples were evaluated for gene expression with the use of GeneChip Human Gene 1.0ST microarrays. Differential expression analysis revealed small subsets of genes that presented positive or negative monotone trends in both the 5th (138 genes) and 28th DOL (308 genes) in the three subgroups of patients. Based on pathway enrichment analysis, we found that most of the pathways that revealed a positive monotone trend were involved in host immunity. The most significantly GA dependent pathways were T-cell receptor signaling pathway and intestinal immune network for IgA production. Overall 4431 genes were differentially expressed between the 5th and 28th DOL. Despite differences in gestational age, patients with the same postconceptional age have a very similar expression of genes.
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spelling pubmed-40584912014-06-30 Development and Maturation of the Immune System in Preterm Neonates: Results from a Whole Genome Expression Study Zasada, Magdalena Kwinta, Przemko Durlak, Wojciech Bik-Multanowski, Mirosław Madetko-Talowska, Anna Pietrzyk, Jacek Józef Biomed Res Int Research Article To expand the knowledge about the consecutive expression of genes involved in the immune system development in preterm neonates and to verify if the environment changes the gene expression after birth we conducted a prospective study that included three cohorts: (A) extremely (gestational age (GA): 23–26 weeks; n = 41), (B) very (GA: 27–29 weeks; n = 39), and (C) moderately preterm infants (GA: 30–32 weeks; n = 33). Blood samples were drawn from the study participants on the 5th and 28th day of life (DOL). The mRNA samples were evaluated for gene expression with the use of GeneChip Human Gene 1.0ST microarrays. Differential expression analysis revealed small subsets of genes that presented positive or negative monotone trends in both the 5th (138 genes) and 28th DOL (308 genes) in the three subgroups of patients. Based on pathway enrichment analysis, we found that most of the pathways that revealed a positive monotone trend were involved in host immunity. The most significantly GA dependent pathways were T-cell receptor signaling pathway and intestinal immune network for IgA production. Overall 4431 genes were differentially expressed between the 5th and 28th DOL. Despite differences in gestational age, patients with the same postconceptional age have a very similar expression of genes. Hindawi Publishing Corporation 2014 2014-05-28 /pmc/articles/PMC4058491/ /pubmed/24982884 http://dx.doi.org/10.1155/2014/498318 Text en Copyright © 2014 Magdalena Zasada et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Zasada, Magdalena
Kwinta, Przemko
Durlak, Wojciech
Bik-Multanowski, Mirosław
Madetko-Talowska, Anna
Pietrzyk, Jacek Józef
Development and Maturation of the Immune System in Preterm Neonates: Results from a Whole Genome Expression Study
title Development and Maturation of the Immune System in Preterm Neonates: Results from a Whole Genome Expression Study
title_full Development and Maturation of the Immune System in Preterm Neonates: Results from a Whole Genome Expression Study
title_fullStr Development and Maturation of the Immune System in Preterm Neonates: Results from a Whole Genome Expression Study
title_full_unstemmed Development and Maturation of the Immune System in Preterm Neonates: Results from a Whole Genome Expression Study
title_short Development and Maturation of the Immune System in Preterm Neonates: Results from a Whole Genome Expression Study
title_sort development and maturation of the immune system in preterm neonates: results from a whole genome expression study
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4058491/
https://www.ncbi.nlm.nih.gov/pubmed/24982884
http://dx.doi.org/10.1155/2014/498318
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