Characterisation of the Proteome of Leptospira interrogans Serovar Canicola as a Resource for the Identification of Common Serovar Immunogenic Proteins

Over 230 serovars of Leptospira interrogans have been identified; however few have been completely characterised. The aim of this study was to characterise the proteome of serovar Canicola and to compare this against the serovars of Copenhageni and Pomona. 2D-LC/MS analysis identified 1653 Leptospir...

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Detalles Bibliográficos
Autores principales: Humphryes, P. C., Weeks, M. E., Coldham, N. G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2014
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4058600/
https://www.ncbi.nlm.nih.gov/pubmed/24987528
http://dx.doi.org/10.1155/2014/572901
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author Humphryes, P. C.
Weeks, M. E.
Coldham, N. G.
author_facet Humphryes, P. C.
Weeks, M. E.
Coldham, N. G.
author_sort Humphryes, P. C.
collection PubMed
description Over 230 serovars of Leptospira interrogans have been identified; however few have been completely characterised. The aim of this study was to characterise the proteome of serovar Canicola and to compare this against the serovars of Copenhageni and Pomona. 2D-LC/MS analysis identified 1653 Leptospira proteins in serovar Canicola; 60 of these proteins were common to Copenhageni and Pomona, 16 of which are known to be immunogenic. This study provides the first reported proteome for serovar Canicola and suggests that proteomic comparison of different serovars could be used as a tool for identification of novel target molecules for vaccine development.
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spelling pubmed-40586002014-07-01 Characterisation of the Proteome of Leptospira interrogans Serovar Canicola as a Resource for the Identification of Common Serovar Immunogenic Proteins Humphryes, P. C. Weeks, M. E. Coldham, N. G. Int J Proteomics Research Article Over 230 serovars of Leptospira interrogans have been identified; however few have been completely characterised. The aim of this study was to characterise the proteome of serovar Canicola and to compare this against the serovars of Copenhageni and Pomona. 2D-LC/MS analysis identified 1653 Leptospira proteins in serovar Canicola; 60 of these proteins were common to Copenhageni and Pomona, 16 of which are known to be immunogenic. This study provides the first reported proteome for serovar Canicola and suggests that proteomic comparison of different serovars could be used as a tool for identification of novel target molecules for vaccine development. Hindawi Publishing Corporation 2014 2014-05-27 /pmc/articles/PMC4058600/ /pubmed/24987528 http://dx.doi.org/10.1155/2014/572901 Text en Copyright © 2014 P. C. Humphryes et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Humphryes, P. C.
Weeks, M. E.
Coldham, N. G.
Characterisation of the Proteome of Leptospira interrogans Serovar Canicola as a Resource for the Identification of Common Serovar Immunogenic Proteins
title Characterisation of the Proteome of Leptospira interrogans Serovar Canicola as a Resource for the Identification of Common Serovar Immunogenic Proteins
title_full Characterisation of the Proteome of Leptospira interrogans Serovar Canicola as a Resource for the Identification of Common Serovar Immunogenic Proteins
title_fullStr Characterisation of the Proteome of Leptospira interrogans Serovar Canicola as a Resource for the Identification of Common Serovar Immunogenic Proteins
title_full_unstemmed Characterisation of the Proteome of Leptospira interrogans Serovar Canicola as a Resource for the Identification of Common Serovar Immunogenic Proteins
title_short Characterisation of the Proteome of Leptospira interrogans Serovar Canicola as a Resource for the Identification of Common Serovar Immunogenic Proteins
title_sort characterisation of the proteome of leptospira interrogans serovar canicola as a resource for the identification of common serovar immunogenic proteins
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4058600/
https://www.ncbi.nlm.nih.gov/pubmed/24987528
http://dx.doi.org/10.1155/2014/572901
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