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Behavioural screening of zebrafish using neuroactive traditional Chinese medicine prescriptions and biological targets

The mechanism of the therapeutic action of antidepressants remains uncertain in traditional Chinese medicine (TCM). In this study, we selected 7 classical TCM prescriptions and utilised an automatic video-tracking system to monitor the rest/wake behaviour of larval zebrafish at 4 days post-fertilisa...

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Autores principales: Wang, Ya-Nan, Hou, Yuan-Yuan, Sun, Ming-Zhu, Zhang, Chun-Yang, Bai, Gang, Zhao, Xin, Feng, Xi-Zeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4058885/
https://www.ncbi.nlm.nih.gov/pubmed/24931174
http://dx.doi.org/10.1038/srep05311
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author Wang, Ya-Nan
Hou, Yuan-Yuan
Sun, Ming-Zhu
Zhang, Chun-Yang
Bai, Gang
Zhao, Xin
Feng, Xi-Zeng
author_facet Wang, Ya-Nan
Hou, Yuan-Yuan
Sun, Ming-Zhu
Zhang, Chun-Yang
Bai, Gang
Zhao, Xin
Feng, Xi-Zeng
author_sort Wang, Ya-Nan
collection PubMed
description The mechanism of the therapeutic action of antidepressants remains uncertain in traditional Chinese medicine (TCM). In this study, we selected 7 classical TCM prescriptions and utilised an automatic video-tracking system to monitor the rest/wake behaviour of larval zebrafish at 4 days post-fertilisation (dpf) for 48 hours. We found that the curative effects of the prescriptions were dose-dependent. K-means clustering was performed according to the shared behavioural phenotypes of the zebrafish. The results revealed that the rest/wake behavioural profiles induced by the same class of prescriptions were similar. A correlation analysis was conducted between the TCM prescriptions and the known compounds. The results showed that the TCM prescriptions correlated well with some well-known compounds. Therefore, we predicted that they may share a similar mechanism of action. This paper describes the first study to combine TCM research with zebrafish rest/wake behaviour in vivo and presents a powerful approach for the discovery of the mechanism of action of TCM prescriptions.
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spelling pubmed-40588852014-06-16 Behavioural screening of zebrafish using neuroactive traditional Chinese medicine prescriptions and biological targets Wang, Ya-Nan Hou, Yuan-Yuan Sun, Ming-Zhu Zhang, Chun-Yang Bai, Gang Zhao, Xin Feng, Xi-Zeng Sci Rep Article The mechanism of the therapeutic action of antidepressants remains uncertain in traditional Chinese medicine (TCM). In this study, we selected 7 classical TCM prescriptions and utilised an automatic video-tracking system to monitor the rest/wake behaviour of larval zebrafish at 4 days post-fertilisation (dpf) for 48 hours. We found that the curative effects of the prescriptions were dose-dependent. K-means clustering was performed according to the shared behavioural phenotypes of the zebrafish. The results revealed that the rest/wake behavioural profiles induced by the same class of prescriptions were similar. A correlation analysis was conducted between the TCM prescriptions and the known compounds. The results showed that the TCM prescriptions correlated well with some well-known compounds. Therefore, we predicted that they may share a similar mechanism of action. This paper describes the first study to combine TCM research with zebrafish rest/wake behaviour in vivo and presents a powerful approach for the discovery of the mechanism of action of TCM prescriptions. Nature Publishing Group 2014-06-16 /pmc/articles/PMC4058885/ /pubmed/24931174 http://dx.doi.org/10.1038/srep05311 Text en Copyright © 2014, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by-nc-nd/4.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/
spellingShingle Article
Wang, Ya-Nan
Hou, Yuan-Yuan
Sun, Ming-Zhu
Zhang, Chun-Yang
Bai, Gang
Zhao, Xin
Feng, Xi-Zeng
Behavioural screening of zebrafish using neuroactive traditional Chinese medicine prescriptions and biological targets
title Behavioural screening of zebrafish using neuroactive traditional Chinese medicine prescriptions and biological targets
title_full Behavioural screening of zebrafish using neuroactive traditional Chinese medicine prescriptions and biological targets
title_fullStr Behavioural screening of zebrafish using neuroactive traditional Chinese medicine prescriptions and biological targets
title_full_unstemmed Behavioural screening of zebrafish using neuroactive traditional Chinese medicine prescriptions and biological targets
title_short Behavioural screening of zebrafish using neuroactive traditional Chinese medicine prescriptions and biological targets
title_sort behavioural screening of zebrafish using neuroactive traditional chinese medicine prescriptions and biological targets
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4058885/
https://www.ncbi.nlm.nih.gov/pubmed/24931174
http://dx.doi.org/10.1038/srep05311
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