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Early quantification of the therapeutic efficacy of the vascular disrupting agent, CKD-516, using dynamic contrast-enhanced ultrasonography in rabbit VX2 liver tumors
PURPOSE: To evaluate the usefulness of dynamic contrast-enhanced ultrasonography (DCE-US) in the early quantification of hemodynamic change following administration of the vascular disrupting agent (VDA) CKD-516 using a rabbit VX2 liver tumor model. METHODS: This study was approved by our institutio...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Korean Society of Ultrasound in Medicine
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4058966/ https://www.ncbi.nlm.nih.gov/pubmed/24936491 http://dx.doi.org/10.14366/usg.13006 |
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author | Joo, Ijin Kim, Jung Hoon Lee, Jeong Min Choi, Jin Woo Han, Joon Koo Choi, Byung Ihn |
author_facet | Joo, Ijin Kim, Jung Hoon Lee, Jeong Min Choi, Jin Woo Han, Joon Koo Choi, Byung Ihn |
author_sort | Joo, Ijin |
collection | PubMed |
description | PURPOSE: To evaluate the usefulness of dynamic contrast-enhanced ultrasonography (DCE-US) in the early quantification of hemodynamic change following administration of the vascular disrupting agent (VDA) CKD-516 using a rabbit VX2 liver tumor model. METHODS: This study was approved by our institutional animal care and use committee. Eight VX2 liver-tumor-bearing rabbits were treated with intravenous CKD-516, and all underwent DCE-US using SonoVue before and again 2, 4, 6, and 24 hours following their treatment. The tumor perfusion parameters were obtained from the time-intensity curve of the DCE-US data. Repeated measures analysis of variance was performed to assess any significant change in tumor perfusion over time. Relative changes in the DCE-US parameters between the baseline and follow-up assessments were correlated with the relative changes in tumor size over the course of seven days using Pearson correlation. RESULTS: CKD-516 treatment resulted in significant changes in the DCE-US parameters, including the peak intensity, total area under the time-intensity curve (AUC(total)), and AUC during wash-out (AUC(out)) over time (P<0.05). Pairwise comparison tests revealed that the AUC(total) and AUC during wash-in (AUC(in)) seen on the two-hour follow-up were significantly lower than the baseline values (P<0.05). However, none of early changes in the DCE-US parameters until 24-hour follow-up showed a significant correlation with the relative changes in tumor size during seven days after CKD-516 treatment. CONCLUSION: Our results suggest that a novel VDA (CKD-516) can cause disruption of tumor perfusion as early as two hours after treatment and that the therapeutic effect of CKD-516 treatment can be effectively quantified using DCE-US. |
format | Online Article Text |
id | pubmed-4058966 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Korean Society of Ultrasound in Medicine |
record_format | MEDLINE/PubMed |
spelling | pubmed-40589662014-06-16 Early quantification of the therapeutic efficacy of the vascular disrupting agent, CKD-516, using dynamic contrast-enhanced ultrasonography in rabbit VX2 liver tumors Joo, Ijin Kim, Jung Hoon Lee, Jeong Min Choi, Jin Woo Han, Joon Koo Choi, Byung Ihn Ultrasonography Original Article PURPOSE: To evaluate the usefulness of dynamic contrast-enhanced ultrasonography (DCE-US) in the early quantification of hemodynamic change following administration of the vascular disrupting agent (VDA) CKD-516 using a rabbit VX2 liver tumor model. METHODS: This study was approved by our institutional animal care and use committee. Eight VX2 liver-tumor-bearing rabbits were treated with intravenous CKD-516, and all underwent DCE-US using SonoVue before and again 2, 4, 6, and 24 hours following their treatment. The tumor perfusion parameters were obtained from the time-intensity curve of the DCE-US data. Repeated measures analysis of variance was performed to assess any significant change in tumor perfusion over time. Relative changes in the DCE-US parameters between the baseline and follow-up assessments were correlated with the relative changes in tumor size over the course of seven days using Pearson correlation. RESULTS: CKD-516 treatment resulted in significant changes in the DCE-US parameters, including the peak intensity, total area under the time-intensity curve (AUC(total)), and AUC during wash-out (AUC(out)) over time (P<0.05). Pairwise comparison tests revealed that the AUC(total) and AUC during wash-in (AUC(in)) seen on the two-hour follow-up were significantly lower than the baseline values (P<0.05). However, none of early changes in the DCE-US parameters until 24-hour follow-up showed a significant correlation with the relative changes in tumor size during seven days after CKD-516 treatment. CONCLUSION: Our results suggest that a novel VDA (CKD-516) can cause disruption of tumor perfusion as early as two hours after treatment and that the therapeutic effect of CKD-516 treatment can be effectively quantified using DCE-US. Korean Society of Ultrasound in Medicine 2014-01 2013-11-15 /pmc/articles/PMC4058966/ /pubmed/24936491 http://dx.doi.org/10.14366/usg.13006 Text en Copyright © 2014 Korean Society of Ultrasound in Medicine (KSUM) This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Joo, Ijin Kim, Jung Hoon Lee, Jeong Min Choi, Jin Woo Han, Joon Koo Choi, Byung Ihn Early quantification of the therapeutic efficacy of the vascular disrupting agent, CKD-516, using dynamic contrast-enhanced ultrasonography in rabbit VX2 liver tumors |
title | Early quantification of the therapeutic efficacy of the vascular
disrupting agent, CKD-516, using dynamic contrast-enhanced ultrasonography in
rabbit VX2 liver tumors |
title_full | Early quantification of the therapeutic efficacy of the vascular
disrupting agent, CKD-516, using dynamic contrast-enhanced ultrasonography in
rabbit VX2 liver tumors |
title_fullStr | Early quantification of the therapeutic efficacy of the vascular
disrupting agent, CKD-516, using dynamic contrast-enhanced ultrasonography in
rabbit VX2 liver tumors |
title_full_unstemmed | Early quantification of the therapeutic efficacy of the vascular
disrupting agent, CKD-516, using dynamic contrast-enhanced ultrasonography in
rabbit VX2 liver tumors |
title_short | Early quantification of the therapeutic efficacy of the vascular
disrupting agent, CKD-516, using dynamic contrast-enhanced ultrasonography in
rabbit VX2 liver tumors |
title_sort | early quantification of the therapeutic efficacy of the vascular
disrupting agent, ckd-516, using dynamic contrast-enhanced ultrasonography in
rabbit vx2 liver tumors |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4058966/ https://www.ncbi.nlm.nih.gov/pubmed/24936491 http://dx.doi.org/10.14366/usg.13006 |
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