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Methods to preserve potentially toxigenic fungi
Microorganisms are a source of many high-value compounds which are useful to every living being, such as humans, plants and animals. Since the process of isolating and improving a microorganism can be lengthy and expensive, preserving the obtained characteristic is of paramount importance, so the pr...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Sociedade Brasileira de Microbiologia
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4059324/ https://www.ncbi.nlm.nih.gov/pubmed/24948912 |
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author | Guimarães, Lucas Costa Fernandes, Ana Paula Chalfoun, Sara Maria Batista, Luís Roberto |
author_facet | Guimarães, Lucas Costa Fernandes, Ana Paula Chalfoun, Sara Maria Batista, Luís Roberto |
author_sort | Guimarães, Lucas Costa |
collection | PubMed |
description | Microorganisms are a source of many high-value compounds which are useful to every living being, such as humans, plants and animals. Since the process of isolating and improving a microorganism can be lengthy and expensive, preserving the obtained characteristic is of paramount importance, so the process does not need to be repeated. Fungi are eukaryotic, achlorophyllous, heterotrophic organisms, usually filamentous, absorb their food, can be either macro or microscopic, propagate themselves by means of spores and store glycogen as a source of storage. Fungi, while infesting food, may produce toxic substances such as mycotoxins. The great genetic diversity of the Kingdom Fungi renders the preservation of fungal cultures for many years relevant. Several international reference mycological culture collections are maintained in many countries. The methodologies that are most fit for preserving microorganisms for extended periods are based on lowering the metabolism until it reaches a stage of artificial dormancy. The goal of this study was to analyze three methods for potentially toxigenic fungal conservation (Castellani’s, continuous subculture and lyophilization) and to identify the best among them. |
format | Online Article Text |
id | pubmed-4059324 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Sociedade Brasileira de Microbiologia |
record_format | MEDLINE/PubMed |
spelling | pubmed-40593242014-06-19 Methods to preserve potentially toxigenic fungi Guimarães, Lucas Costa Fernandes, Ana Paula Chalfoun, Sara Maria Batista, Luís Roberto Braz J Microbiol Research Paper Microorganisms are a source of many high-value compounds which are useful to every living being, such as humans, plants and animals. Since the process of isolating and improving a microorganism can be lengthy and expensive, preserving the obtained characteristic is of paramount importance, so the process does not need to be repeated. Fungi are eukaryotic, achlorophyllous, heterotrophic organisms, usually filamentous, absorb their food, can be either macro or microscopic, propagate themselves by means of spores and store glycogen as a source of storage. Fungi, while infesting food, may produce toxic substances such as mycotoxins. The great genetic diversity of the Kingdom Fungi renders the preservation of fungal cultures for many years relevant. Several international reference mycological culture collections are maintained in many countries. The methodologies that are most fit for preserving microorganisms for extended periods are based on lowering the metabolism until it reaches a stage of artificial dormancy. The goal of this study was to analyze three methods for potentially toxigenic fungal conservation (Castellani’s, continuous subculture and lyophilization) and to identify the best among them. Sociedade Brasileira de Microbiologia 2014-05-19 /pmc/articles/PMC4059324/ /pubmed/24948912 Text en Copyright © 2014, Sociedade Brasileira de Microbiologia All the content of the journal, except where otherwise noted, is licensed under a Creative Commons License CC BY-NC. |
spellingShingle | Research Paper Guimarães, Lucas Costa Fernandes, Ana Paula Chalfoun, Sara Maria Batista, Luís Roberto Methods to preserve potentially toxigenic fungi |
title | Methods to preserve potentially toxigenic fungi |
title_full | Methods to preserve potentially toxigenic fungi |
title_fullStr | Methods to preserve potentially toxigenic fungi |
title_full_unstemmed | Methods to preserve potentially toxigenic fungi |
title_short | Methods to preserve potentially toxigenic fungi |
title_sort | methods to preserve potentially toxigenic fungi |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4059324/ https://www.ncbi.nlm.nih.gov/pubmed/24948912 |
work_keys_str_mv | AT guimaraeslucascosta methodstopreservepotentiallytoxigenicfungi AT fernandesanapaula methodstopreservepotentiallytoxigenicfungi AT chalfounsaramaria methodstopreservepotentiallytoxigenicfungi AT batistaluisroberto methodstopreservepotentiallytoxigenicfungi |