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PCR and ELISA (VIDAS ECO O157(®)) Escherichia coli O157:H7 identification in Minas Frescal cheese commercialized in Goiânia, GO
Escherichia coli O157:H7 has been incriminated in food poisoning outbreaks and sporadic cases of hemorrhagic colitis and hemolytic uremic syndrome in many countries. Considering the high susceptibility of Minas Frescal cheese to contamination by E. coli O157:H7, the aim of this study was to determin...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Sociedade Brasileira de Microbiologia
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4059328/ https://www.ncbi.nlm.nih.gov/pubmed/24948907 |
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author | Carvalho, Rosangela Nunes de Oliveira, Antonio Nonato de Mesquita, Albenones José Minafra e Rezende, Cíntia Silva de Mesquita, Adriano Queiroz Romero, Rolando Alfredo Mazzoni |
author_facet | Carvalho, Rosangela Nunes de Oliveira, Antonio Nonato de Mesquita, Albenones José Minafra e Rezende, Cíntia Silva de Mesquita, Adriano Queiroz Romero, Rolando Alfredo Mazzoni |
author_sort | Carvalho, Rosangela Nunes |
collection | PubMed |
description | Escherichia coli O157:H7 has been incriminated in food poisoning outbreaks and sporadic cases of hemorrhagic colitis and hemolytic uremic syndrome in many countries. Considering the high susceptibility of Minas Frescal cheese to contamination by E. coli O157:H7, the aim of this study was to determine the occurrence of this pathogen through PCR (Polymerase Chain Reaction) and ELISA (VIDAS ECO O157(®), bioMérieux, Lyon, France) test. Thirty cheese samples manufactured by artisan farmhouse producers were collected from open-air markets in Goiânia and thirty from industries under Federal Inspection located in Goiás State which trade their products in supermarkets in Goiânia. E. coli O157:H7 was detected in 6.67% samples collected in open air markets using ELISA, and 23,33% with PCR. The pathogen was not detected in samples from industries under Federal Inspection. |
format | Online Article Text |
id | pubmed-4059328 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Sociedade Brasileira de Microbiologia |
record_format | MEDLINE/PubMed |
spelling | pubmed-40593282014-06-19 PCR and ELISA (VIDAS ECO O157(®)) Escherichia coli O157:H7 identification in Minas Frescal cheese commercialized in Goiânia, GO Carvalho, Rosangela Nunes de Oliveira, Antonio Nonato de Mesquita, Albenones José Minafra e Rezende, Cíntia Silva de Mesquita, Adriano Queiroz Romero, Rolando Alfredo Mazzoni Braz J Microbiol Research Paper Escherichia coli O157:H7 has been incriminated in food poisoning outbreaks and sporadic cases of hemorrhagic colitis and hemolytic uremic syndrome in many countries. Considering the high susceptibility of Minas Frescal cheese to contamination by E. coli O157:H7, the aim of this study was to determine the occurrence of this pathogen through PCR (Polymerase Chain Reaction) and ELISA (VIDAS ECO O157(®), bioMérieux, Lyon, France) test. Thirty cheese samples manufactured by artisan farmhouse producers were collected from open-air markets in Goiânia and thirty from industries under Federal Inspection located in Goiás State which trade their products in supermarkets in Goiânia. E. coli O157:H7 was detected in 6.67% samples collected in open air markets using ELISA, and 23,33% with PCR. The pathogen was not detected in samples from industries under Federal Inspection. Sociedade Brasileira de Microbiologia 2014-05-19 /pmc/articles/PMC4059328/ /pubmed/24948907 Text en Copyright © 2014, Sociedade Brasileira de Microbiologia All the content of the journal, except where otherwise noted, is licensed under a Creative Commons License CC BY-NC. |
spellingShingle | Research Paper Carvalho, Rosangela Nunes de Oliveira, Antonio Nonato de Mesquita, Albenones José Minafra e Rezende, Cíntia Silva de Mesquita, Adriano Queiroz Romero, Rolando Alfredo Mazzoni PCR and ELISA (VIDAS ECO O157(®)) Escherichia coli O157:H7 identification in Minas Frescal cheese commercialized in Goiânia, GO |
title | PCR and ELISA (VIDAS ECO O157(®)) Escherichia coli O157:H7 identification in Minas Frescal cheese commercialized in Goiânia, GO |
title_full | PCR and ELISA (VIDAS ECO O157(®)) Escherichia coli O157:H7 identification in Minas Frescal cheese commercialized in Goiânia, GO |
title_fullStr | PCR and ELISA (VIDAS ECO O157(®)) Escherichia coli O157:H7 identification in Minas Frescal cheese commercialized in Goiânia, GO |
title_full_unstemmed | PCR and ELISA (VIDAS ECO O157(®)) Escherichia coli O157:H7 identification in Minas Frescal cheese commercialized in Goiânia, GO |
title_short | PCR and ELISA (VIDAS ECO O157(®)) Escherichia coli O157:H7 identification in Minas Frescal cheese commercialized in Goiânia, GO |
title_sort | pcr and elisa (vidas eco o157(®)) escherichia coli o157:h7 identification in minas frescal cheese commercialized in goiânia, go |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4059328/ https://www.ncbi.nlm.nih.gov/pubmed/24948907 |
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