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Dysbiosis of Gut Fungal Microbiota is Associated With Mucosal Inflammation in Crohn’s Disease

GOALS: We aim to characterize the fungal microbiota in the intestinal mucosa and feces in patients with Crohn’s disease (CD). BACKGROUND: Fungi represent a diverse microbial community in the human intestine and might play a role in the pathogenesis of CD; however, little is known about the structure...

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Autores principales: Li, Qiurong, Wang, Chenyang, Tang, Chun, He, Qin, Li, Ning, Li, Jieshou
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Raven Press 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4059552/
https://www.ncbi.nlm.nih.gov/pubmed/24275714
http://dx.doi.org/10.1097/MCG.0000000000000035
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author Li, Qiurong
Wang, Chenyang
Tang, Chun
He, Qin
Li, Ning
Li, Jieshou
author_facet Li, Qiurong
Wang, Chenyang
Tang, Chun
He, Qin
Li, Ning
Li, Jieshou
author_sort Li, Qiurong
collection PubMed
description GOALS: We aim to characterize the fungal microbiota in the intestinal mucosa and feces in patients with Crohn’s disease (CD). BACKGROUND: Fungi represent a diverse microbial community in the human intestine and might play a role in the pathogenesis of CD; however, little is known about the structure and composition of the fungal microbiota especially adhering to the intestinal mucosa in CD patient. STUDY: Nineteen patients with active CD and 7 healthy individuals were recruited in this study. The mucosa-associated and fecal fungal microbiotas in CD patients were analyzed using culture-independent community fingerprint techniques. RESULTS: The fungal richness and diversity were significantly elevated in the inflamed mucosa compared with the noninflamed mucosa. The predominant fungal composition in the inflamed mucosa was strikingly altered, mainly characterized by expansion in the proportions of Candida spp., Gibberella moniliformis, Alternaria brassicicola, and Cryptococcus neoformans. The fecal fungal community was perturbed in CD patients as accompanied by increased fungal diversity and prevalence in Candida albicans, Aspergillus clavatus, and C. neoformans. The species richness and diversity of the mucosal fungal community were associated with the expression of TNF-α, IFN-γ, or IL-10 (P<0.05). The diversity of the fecal fungal microbiota positively correlated with serum C-reactive protein and CD activity index (P<0.05). CONCLUSIONS: This study first demonstrates that the fungal microbiota in the inflamed mucosa is distinguishable from that of the noninflamed area. Shifts of gut fungal microbiota composition may be associated with mucosal inflammation and disease activity of CD. Our data would provide novel insights into understanding the potential of gut fungal microbiota in the pathogenesis of CD.
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spelling pubmed-40595522014-06-17 Dysbiosis of Gut Fungal Microbiota is Associated With Mucosal Inflammation in Crohn’s Disease Li, Qiurong Wang, Chenyang Tang, Chun He, Qin Li, Ning Li, Jieshou J Clin Gastroenterol ALIMENTARY TRACT: Original Articles GOALS: We aim to characterize the fungal microbiota in the intestinal mucosa and feces in patients with Crohn’s disease (CD). BACKGROUND: Fungi represent a diverse microbial community in the human intestine and might play a role in the pathogenesis of CD; however, little is known about the structure and composition of the fungal microbiota especially adhering to the intestinal mucosa in CD patient. STUDY: Nineteen patients with active CD and 7 healthy individuals were recruited in this study. The mucosa-associated and fecal fungal microbiotas in CD patients were analyzed using culture-independent community fingerprint techniques. RESULTS: The fungal richness and diversity were significantly elevated in the inflamed mucosa compared with the noninflamed mucosa. The predominant fungal composition in the inflamed mucosa was strikingly altered, mainly characterized by expansion in the proportions of Candida spp., Gibberella moniliformis, Alternaria brassicicola, and Cryptococcus neoformans. The fecal fungal community was perturbed in CD patients as accompanied by increased fungal diversity and prevalence in Candida albicans, Aspergillus clavatus, and C. neoformans. The species richness and diversity of the mucosal fungal community were associated with the expression of TNF-α, IFN-γ, or IL-10 (P<0.05). The diversity of the fecal fungal microbiota positively correlated with serum C-reactive protein and CD activity index (P<0.05). CONCLUSIONS: This study first demonstrates that the fungal microbiota in the inflamed mucosa is distinguishable from that of the noninflamed area. Shifts of gut fungal microbiota composition may be associated with mucosal inflammation and disease activity of CD. Our data would provide novel insights into understanding the potential of gut fungal microbiota in the pathogenesis of CD. Raven Press 2014-07 2014-06-24 /pmc/articles/PMC4059552/ /pubmed/24275714 http://dx.doi.org/10.1097/MCG.0000000000000035 Text en Copyright © 2013 by Lippincott Williams & Wilkins http://creativecommons.org/licenses/by-nc-nd/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivitives 3.0 License, where it is permissible to download and share the work provided it is properly cited. The work cannot be changed in any way or used commercially.
spellingShingle ALIMENTARY TRACT: Original Articles
Li, Qiurong
Wang, Chenyang
Tang, Chun
He, Qin
Li, Ning
Li, Jieshou
Dysbiosis of Gut Fungal Microbiota is Associated With Mucosal Inflammation in Crohn’s Disease
title Dysbiosis of Gut Fungal Microbiota is Associated With Mucosal Inflammation in Crohn’s Disease
title_full Dysbiosis of Gut Fungal Microbiota is Associated With Mucosal Inflammation in Crohn’s Disease
title_fullStr Dysbiosis of Gut Fungal Microbiota is Associated With Mucosal Inflammation in Crohn’s Disease
title_full_unstemmed Dysbiosis of Gut Fungal Microbiota is Associated With Mucosal Inflammation in Crohn’s Disease
title_short Dysbiosis of Gut Fungal Microbiota is Associated With Mucosal Inflammation in Crohn’s Disease
title_sort dysbiosis of gut fungal microbiota is associated with mucosal inflammation in crohn’s disease
topic ALIMENTARY TRACT: Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4059552/
https://www.ncbi.nlm.nih.gov/pubmed/24275714
http://dx.doi.org/10.1097/MCG.0000000000000035
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