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Tools and methods for studying Notch signaling in Drosophila melanogaster

Notch signaling involves a highly conserved pathway that mediates communication between neighboring cells. Activation of Notch by its ligands, results in the release of the Notch intracellular domain (NICD), which enters the nucleus and regulates transcription. This pathway has been implicated in ma...

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Detalles Bibliográficos
Autores principales: Zacharioudaki, Evanthia, Bray, Sarah J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Academic Press 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4059942/
https://www.ncbi.nlm.nih.gov/pubmed/24704358
http://dx.doi.org/10.1016/j.ymeth.2014.03.029
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author Zacharioudaki, Evanthia
Bray, Sarah J.
author_facet Zacharioudaki, Evanthia
Bray, Sarah J.
author_sort Zacharioudaki, Evanthia
collection PubMed
description Notch signaling involves a highly conserved pathway that mediates communication between neighboring cells. Activation of Notch by its ligands, results in the release of the Notch intracellular domain (NICD), which enters the nucleus and regulates transcription. This pathway has been implicated in many developmental decisions and diseases (including cancers) over the past decades. The simplicity of the Notch pathway in Drosophila melanogaster, in combination with the availability of powerful genetics, make this an attractive model for studying fundamental principles of Notch regulation and function. In this article we present some of the established and emerging tools that are available to monitor and manipulate the Notch pathway in Drosophila and discuss their strengths and weaknesses.
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spelling pubmed-40599422014-06-18 Tools and methods for studying Notch signaling in Drosophila melanogaster Zacharioudaki, Evanthia Bray, Sarah J. Methods Article Notch signaling involves a highly conserved pathway that mediates communication between neighboring cells. Activation of Notch by its ligands, results in the release of the Notch intracellular domain (NICD), which enters the nucleus and regulates transcription. This pathway has been implicated in many developmental decisions and diseases (including cancers) over the past decades. The simplicity of the Notch pathway in Drosophila melanogaster, in combination with the availability of powerful genetics, make this an attractive model for studying fundamental principles of Notch regulation and function. In this article we present some of the established and emerging tools that are available to monitor and manipulate the Notch pathway in Drosophila and discuss their strengths and weaknesses. Academic Press 2014-06-15 /pmc/articles/PMC4059942/ /pubmed/24704358 http://dx.doi.org/10.1016/j.ymeth.2014.03.029 Text en © 2014 The Authors http://creativecommons.org/licenses/by/3.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Zacharioudaki, Evanthia
Bray, Sarah J.
Tools and methods for studying Notch signaling in Drosophila melanogaster
title Tools and methods for studying Notch signaling in Drosophila melanogaster
title_full Tools and methods for studying Notch signaling in Drosophila melanogaster
title_fullStr Tools and methods for studying Notch signaling in Drosophila melanogaster
title_full_unstemmed Tools and methods for studying Notch signaling in Drosophila melanogaster
title_short Tools and methods for studying Notch signaling in Drosophila melanogaster
title_sort tools and methods for studying notch signaling in drosophila melanogaster
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4059942/
https://www.ncbi.nlm.nih.gov/pubmed/24704358
http://dx.doi.org/10.1016/j.ymeth.2014.03.029
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