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Regulatory T Cells Protect Fine Particulate Matter-Induced Inflammatory Responses in Human Umbilical Vein Endothelial Cells

Objective. To investigate the role of CD4(+)CD25(+) T cells (Tregs) in protecting fine particulate matter (PM-) induced inflammatory responses, and its potential mechanisms. Methods. Human umbilical vein endothelial cells (HUVECs) were treated with graded concentrations (2, 5, 10, 20, and 40 µg/cm(2...

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Autores principales: Zhang, Wen-cai, Wang, Yan-ge, Zhu, Zheng-feng, Wu, Fang-qin, Peng, Yu-dong, Chen, Zhu-yue, Yang, Jin-hua, Wu, Jing-jing, Lian, Yi-tian, He, Mei-an, Wu, Tang-chun, Cheng, Long-xian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4060066/
https://www.ncbi.nlm.nih.gov/pubmed/24987196
http://dx.doi.org/10.1155/2014/869148
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author Zhang, Wen-cai
Wang, Yan-ge
Zhu, Zheng-feng
Wu, Fang-qin
Peng, Yu-dong
Chen, Zhu-yue
Yang, Jin-hua
Wu, Jing-jing
Lian, Yi-tian
He, Mei-an
Wu, Tang-chun
Cheng, Long-xian
author_facet Zhang, Wen-cai
Wang, Yan-ge
Zhu, Zheng-feng
Wu, Fang-qin
Peng, Yu-dong
Chen, Zhu-yue
Yang, Jin-hua
Wu, Jing-jing
Lian, Yi-tian
He, Mei-an
Wu, Tang-chun
Cheng, Long-xian
author_sort Zhang, Wen-cai
collection PubMed
description Objective. To investigate the role of CD4(+)CD25(+) T cells (Tregs) in protecting fine particulate matter (PM-) induced inflammatory responses, and its potential mechanisms. Methods. Human umbilical vein endothelial cells (HUVECs) were treated with graded concentrations (2, 5, 10, 20, and 40 µg/cm(2)) of suspension of fine particles for 24h. For coculture experiment, HUVECs were incubated alone, with CD4(+)CD25(−) T cells (Teff), or with Tregs in the presence of anti-CD3 monoclonal antibodies for 48 hours, and then were stimulated with or without suspension of fine particles for 24 hours. The expression of adhesion molecules and inflammatory cytokines was examined. Results. Adhesion molecules, including vascular cell adhesion molecule-1 (VCAM-1) and intercellular adhesion molecule-1 (ICAM-1), and inflammatory cytokines, such as interleukin (IL-) 6 and IL-8, were increased in a concentration-dependent manner. Moreover, the adhesion of human acute monocytic leukemia cells (THP-1) to endothelial cells was increased and NF-κB activity was upregulated in HUVECs after treatment with fine particles. However, after Tregs treatment, fine particles-induced inflammatory responses and NF-κB activation were significantly alleviated. Transwell experiments showed that Treg-mediated suppression of HUVECs inflammatory responses impaired by fine particles required cell contact and soluble factors. Conclusions. Tregs could attenuate fine particles-induced inflammatory responses and NF-κB activation in HUVECs.
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spelling pubmed-40600662014-07-01 Regulatory T Cells Protect Fine Particulate Matter-Induced Inflammatory Responses in Human Umbilical Vein Endothelial Cells Zhang, Wen-cai Wang, Yan-ge Zhu, Zheng-feng Wu, Fang-qin Peng, Yu-dong Chen, Zhu-yue Yang, Jin-hua Wu, Jing-jing Lian, Yi-tian He, Mei-an Wu, Tang-chun Cheng, Long-xian Mediators Inflamm Research Article Objective. To investigate the role of CD4(+)CD25(+) T cells (Tregs) in protecting fine particulate matter (PM-) induced inflammatory responses, and its potential mechanisms. Methods. Human umbilical vein endothelial cells (HUVECs) were treated with graded concentrations (2, 5, 10, 20, and 40 µg/cm(2)) of suspension of fine particles for 24h. For coculture experiment, HUVECs were incubated alone, with CD4(+)CD25(−) T cells (Teff), or with Tregs in the presence of anti-CD3 monoclonal antibodies for 48 hours, and then were stimulated with or without suspension of fine particles for 24 hours. The expression of adhesion molecules and inflammatory cytokines was examined. Results. Adhesion molecules, including vascular cell adhesion molecule-1 (VCAM-1) and intercellular adhesion molecule-1 (ICAM-1), and inflammatory cytokines, such as interleukin (IL-) 6 and IL-8, were increased in a concentration-dependent manner. Moreover, the adhesion of human acute monocytic leukemia cells (THP-1) to endothelial cells was increased and NF-κB activity was upregulated in HUVECs after treatment with fine particles. However, after Tregs treatment, fine particles-induced inflammatory responses and NF-κB activation were significantly alleviated. Transwell experiments showed that Treg-mediated suppression of HUVECs inflammatory responses impaired by fine particles required cell contact and soluble factors. Conclusions. Tregs could attenuate fine particles-induced inflammatory responses and NF-κB activation in HUVECs. Hindawi Publishing Corporation 2014 2014-05-29 /pmc/articles/PMC4060066/ /pubmed/24987196 http://dx.doi.org/10.1155/2014/869148 Text en Copyright © 2014 Wen-cai Zhang et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Zhang, Wen-cai
Wang, Yan-ge
Zhu, Zheng-feng
Wu, Fang-qin
Peng, Yu-dong
Chen, Zhu-yue
Yang, Jin-hua
Wu, Jing-jing
Lian, Yi-tian
He, Mei-an
Wu, Tang-chun
Cheng, Long-xian
Regulatory T Cells Protect Fine Particulate Matter-Induced Inflammatory Responses in Human Umbilical Vein Endothelial Cells
title Regulatory T Cells Protect Fine Particulate Matter-Induced Inflammatory Responses in Human Umbilical Vein Endothelial Cells
title_full Regulatory T Cells Protect Fine Particulate Matter-Induced Inflammatory Responses in Human Umbilical Vein Endothelial Cells
title_fullStr Regulatory T Cells Protect Fine Particulate Matter-Induced Inflammatory Responses in Human Umbilical Vein Endothelial Cells
title_full_unstemmed Regulatory T Cells Protect Fine Particulate Matter-Induced Inflammatory Responses in Human Umbilical Vein Endothelial Cells
title_short Regulatory T Cells Protect Fine Particulate Matter-Induced Inflammatory Responses in Human Umbilical Vein Endothelial Cells
title_sort regulatory t cells protect fine particulate matter-induced inflammatory responses in human umbilical vein endothelial cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4060066/
https://www.ncbi.nlm.nih.gov/pubmed/24987196
http://dx.doi.org/10.1155/2014/869148
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