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Enzymatic Treatment of Specimens before DNA Extraction Directly Influences Molecular Detection of Infectious Agents

INTRODUCTION: Biological samples, pharmaceuticals or food contain proteins, lipids, polymers, ammoniums and macromolecules that alter the detection of infectious agents by DNA amplification techniques (PCR). Moreover the targeted DNA has to be released from the complex cell walls and the compact nuc...

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Detalles Bibliográficos
Autores principales: Goldschmidt, Pablo, Degorge, Sandrine, Merabet, Lilia, Chaumeil, Christine
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4061000/
https://www.ncbi.nlm.nih.gov/pubmed/24936792
http://dx.doi.org/10.1371/journal.pone.0094886