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CCL25/CCR9 Interactions Regulate the Function of iNKT Cells in Oxazolone-Induced Colitis in Mice
BACKGROUND: Natural killer T (NKT) cells share phenotypic and functional properties with both conventional natural killer cells and T cells. These cells might have an important role in the pathogenesis of ulcerative colitis (UC). The interaction of chemokine ligand 25 (CCL25) with chemokine receptor...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4061108/ https://www.ncbi.nlm.nih.gov/pubmed/24936795 http://dx.doi.org/10.1371/journal.pone.0100167 |
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author | Zhu, Siying Bing, Yuntao Wang, Xiaobing Yu, Qiao Wang, Yipeng Xu, Shufang Song, Lu Wang, Xintao Xia, Bing Zhu, Youqing Zhou, Rui |
author_facet | Zhu, Siying Bing, Yuntao Wang, Xiaobing Yu, Qiao Wang, Yipeng Xu, Shufang Song, Lu Wang, Xintao Xia, Bing Zhu, Youqing Zhou, Rui |
author_sort | Zhu, Siying |
collection | PubMed |
description | BACKGROUND: Natural killer T (NKT) cells share phenotypic and functional properties with both conventional natural killer cells and T cells. These cells might have an important role in the pathogenesis of ulcerative colitis (UC). The interaction of chemokine ligand 25 (CCL25) with chemokine receptor 9 (CCR9) is involved in gut-specific migration of leukocytes and induces regulatory T cells (Tregs) to migrate to the intestine in chronic ileitis. METHODOLOGY/FINDINGS: In UC patients, NKT receptor CD161, CCL25, and CCR9 expression levels were evaluated by qRT-PCR. A murine model of oxazolone-induced colitis was induced in BALB/c mice. The mRNA levels of NK1.1, CCL25 and CCR9, and pro-inflammatory cytokines in mice were evaluated. The CCR9 expression on Type I or invariant NKT (iNKT) cells, and the iNKT cells chemotaxis are observed according to flow cytometry. NKT receptor CD161, CCL25 and CCR9 expression levels were significantly increased in UC patients. And, the mRNA expression levels of NK1.1, CCL25 and CCR9 were increased in oxazolone-induced colitis in mice. The production of pro-inflammatory cytokines was significantly increased, especially interleukin 4 (IL-4), IL-10 and IL-13. We observed significantly increased CCR9 expression on iNKT cells. Furthermore, we found an increased iNKT population and enhanced chemotaxis during oxazolone-induced colitis. CONCLUSIONS/SIGNIFICANCE: Our study suggests that CCL25/CCR9 interactions may promote the induction and function of iNKT cells during oxazolone-induced colitis. These findings may have important implications for UC treatment and suggest a role for CCR9 inhibitors. |
format | Online Article Text |
id | pubmed-4061108 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-40611082014-06-20 CCL25/CCR9 Interactions Regulate the Function of iNKT Cells in Oxazolone-Induced Colitis in Mice Zhu, Siying Bing, Yuntao Wang, Xiaobing Yu, Qiao Wang, Yipeng Xu, Shufang Song, Lu Wang, Xintao Xia, Bing Zhu, Youqing Zhou, Rui PLoS One Research Article BACKGROUND: Natural killer T (NKT) cells share phenotypic and functional properties with both conventional natural killer cells and T cells. These cells might have an important role in the pathogenesis of ulcerative colitis (UC). The interaction of chemokine ligand 25 (CCL25) with chemokine receptor 9 (CCR9) is involved in gut-specific migration of leukocytes and induces regulatory T cells (Tregs) to migrate to the intestine in chronic ileitis. METHODOLOGY/FINDINGS: In UC patients, NKT receptor CD161, CCL25, and CCR9 expression levels were evaluated by qRT-PCR. A murine model of oxazolone-induced colitis was induced in BALB/c mice. The mRNA levels of NK1.1, CCL25 and CCR9, and pro-inflammatory cytokines in mice were evaluated. The CCR9 expression on Type I or invariant NKT (iNKT) cells, and the iNKT cells chemotaxis are observed according to flow cytometry. NKT receptor CD161, CCL25 and CCR9 expression levels were significantly increased in UC patients. And, the mRNA expression levels of NK1.1, CCL25 and CCR9 were increased in oxazolone-induced colitis in mice. The production of pro-inflammatory cytokines was significantly increased, especially interleukin 4 (IL-4), IL-10 and IL-13. We observed significantly increased CCR9 expression on iNKT cells. Furthermore, we found an increased iNKT population and enhanced chemotaxis during oxazolone-induced colitis. CONCLUSIONS/SIGNIFICANCE: Our study suggests that CCL25/CCR9 interactions may promote the induction and function of iNKT cells during oxazolone-induced colitis. These findings may have important implications for UC treatment and suggest a role for CCR9 inhibitors. Public Library of Science 2014-06-17 /pmc/articles/PMC4061108/ /pubmed/24936795 http://dx.doi.org/10.1371/journal.pone.0100167 Text en © 2014 Zhu et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Zhu, Siying Bing, Yuntao Wang, Xiaobing Yu, Qiao Wang, Yipeng Xu, Shufang Song, Lu Wang, Xintao Xia, Bing Zhu, Youqing Zhou, Rui CCL25/CCR9 Interactions Regulate the Function of iNKT Cells in Oxazolone-Induced Colitis in Mice |
title | CCL25/CCR9 Interactions Regulate the Function of iNKT Cells in Oxazolone-Induced Colitis in Mice |
title_full | CCL25/CCR9 Interactions Regulate the Function of iNKT Cells in Oxazolone-Induced Colitis in Mice |
title_fullStr | CCL25/CCR9 Interactions Regulate the Function of iNKT Cells in Oxazolone-Induced Colitis in Mice |
title_full_unstemmed | CCL25/CCR9 Interactions Regulate the Function of iNKT Cells in Oxazolone-Induced Colitis in Mice |
title_short | CCL25/CCR9 Interactions Regulate the Function of iNKT Cells in Oxazolone-Induced Colitis in Mice |
title_sort | ccl25/ccr9 interactions regulate the function of inkt cells in oxazolone-induced colitis in mice |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4061108/ https://www.ncbi.nlm.nih.gov/pubmed/24936795 http://dx.doi.org/10.1371/journal.pone.0100167 |
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