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xCELLigence system for real-time label-free monitoring of growth and viability of cell lines from hematological malignancies

The xCELLigence system is a new technological approach that allows the real-time cell analysis of adherent tumor cells. To date, xCELLigence has not been able to monitor the growth or cytotoxicity of nonadherent cells derived from hematological malignancies. The basis of its technology relies on the...

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Autores principales: Martinez-Serra, Jordi, Gutierrez, Antonio, Muñoz-Capó, Saúl, Navarro-Palou, María, Ros, Teresa, Amat, Juan Carlos, Lopez, Bernardo, Marcus, Toni F, Fueyo, Laura, Suquia, Angela G, Gines, Jordi, Rubio, Francisco, Ramos, Rafael, Besalduch, Joan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove Medical Press 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4061162/
https://www.ncbi.nlm.nih.gov/pubmed/24959085
http://dx.doi.org/10.2147/OTT.S62887
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author Martinez-Serra, Jordi
Gutierrez, Antonio
Muñoz-Capó, Saúl
Navarro-Palou, María
Ros, Teresa
Amat, Juan Carlos
Lopez, Bernardo
Marcus, Toni F
Fueyo, Laura
Suquia, Angela G
Gines, Jordi
Rubio, Francisco
Ramos, Rafael
Besalduch, Joan
author_facet Martinez-Serra, Jordi
Gutierrez, Antonio
Muñoz-Capó, Saúl
Navarro-Palou, María
Ros, Teresa
Amat, Juan Carlos
Lopez, Bernardo
Marcus, Toni F
Fueyo, Laura
Suquia, Angela G
Gines, Jordi
Rubio, Francisco
Ramos, Rafael
Besalduch, Joan
author_sort Martinez-Serra, Jordi
collection PubMed
description The xCELLigence system is a new technological approach that allows the real-time cell analysis of adherent tumor cells. To date, xCELLigence has not been able to monitor the growth or cytotoxicity of nonadherent cells derived from hematological malignancies. The basis of its technology relies on the use of culture plates with gold microelectrodes located in their base. We have adapted the methodology described by others to xCELLigence, based on the pre-coating of the cell culture surface with specific substrates, some of which are known to facilitate cell adhesion in the extracellular matrix. Pre-coating of the culture plates with fibronectin, compared to laminin, collagen, or gelatin, significantly induced the adhesion of most of the leukemia/lymphoma cells assayed (Jurkat, L1236, KMH2, and K562). With a fibronectin substrate, nonadherent cells deposited in a monolayer configuration, and consequently, the cell growth and viability were robustly monitored. We further demonstrate the feasibility of xCELLigence for the real-time monitoring of the cytotoxic properties of several antineoplastic agents. In order to validate this technology, the data obtained through real-time cell analysis was compared with that obtained from using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide method. This provides an excellent label-free tool for the screening of drug efficacy in nonadherent cells and discriminates optimal time points for further molecular analysis of cellular events associated with treatments, reducing both time and costs.
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spelling pubmed-40611622014-06-23 xCELLigence system for real-time label-free monitoring of growth and viability of cell lines from hematological malignancies Martinez-Serra, Jordi Gutierrez, Antonio Muñoz-Capó, Saúl Navarro-Palou, María Ros, Teresa Amat, Juan Carlos Lopez, Bernardo Marcus, Toni F Fueyo, Laura Suquia, Angela G Gines, Jordi Rubio, Francisco Ramos, Rafael Besalduch, Joan Onco Targets Ther Original Research The xCELLigence system is a new technological approach that allows the real-time cell analysis of adherent tumor cells. To date, xCELLigence has not been able to monitor the growth or cytotoxicity of nonadherent cells derived from hematological malignancies. The basis of its technology relies on the use of culture plates with gold microelectrodes located in their base. We have adapted the methodology described by others to xCELLigence, based on the pre-coating of the cell culture surface with specific substrates, some of which are known to facilitate cell adhesion in the extracellular matrix. Pre-coating of the culture plates with fibronectin, compared to laminin, collagen, or gelatin, significantly induced the adhesion of most of the leukemia/lymphoma cells assayed (Jurkat, L1236, KMH2, and K562). With a fibronectin substrate, nonadherent cells deposited in a monolayer configuration, and consequently, the cell growth and viability were robustly monitored. We further demonstrate the feasibility of xCELLigence for the real-time monitoring of the cytotoxic properties of several antineoplastic agents. In order to validate this technology, the data obtained through real-time cell analysis was compared with that obtained from using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide method. This provides an excellent label-free tool for the screening of drug efficacy in nonadherent cells and discriminates optimal time points for further molecular analysis of cellular events associated with treatments, reducing both time and costs. Dove Medical Press 2014-06-12 /pmc/articles/PMC4061162/ /pubmed/24959085 http://dx.doi.org/10.2147/OTT.S62887 Text en © 2014 Martinez-Serra et al. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.
spellingShingle Original Research
Martinez-Serra, Jordi
Gutierrez, Antonio
Muñoz-Capó, Saúl
Navarro-Palou, María
Ros, Teresa
Amat, Juan Carlos
Lopez, Bernardo
Marcus, Toni F
Fueyo, Laura
Suquia, Angela G
Gines, Jordi
Rubio, Francisco
Ramos, Rafael
Besalduch, Joan
xCELLigence system for real-time label-free monitoring of growth and viability of cell lines from hematological malignancies
title xCELLigence system for real-time label-free monitoring of growth and viability of cell lines from hematological malignancies
title_full xCELLigence system for real-time label-free monitoring of growth and viability of cell lines from hematological malignancies
title_fullStr xCELLigence system for real-time label-free monitoring of growth and viability of cell lines from hematological malignancies
title_full_unstemmed xCELLigence system for real-time label-free monitoring of growth and viability of cell lines from hematological malignancies
title_short xCELLigence system for real-time label-free monitoring of growth and viability of cell lines from hematological malignancies
title_sort xcelligence system for real-time label-free monitoring of growth and viability of cell lines from hematological malignancies
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4061162/
https://www.ncbi.nlm.nih.gov/pubmed/24959085
http://dx.doi.org/10.2147/OTT.S62887
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