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Reproducibility, accuracy and concordance of Accutrend(®) Plus for measuring circulating lipid concentration in adults
INTRODUCTION: The determination of lipid biomarkers by capillary sampling may be useful in the screening, diagnosis and/or personal management of hyperlipidemia and cardiovascular risk. It remains unclear whether the use of the Accutrend(®) Plus system is appropriate. This study aimed to assess its...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Croatian Society of Medical Biochemistry and Laboratory Medicine
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4062330/ https://www.ncbi.nlm.nih.gov/pubmed/22384524 |
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author | Scafoglieri, Aldo Tresignie, Jonathan Provyn, Steven Clarys, Jan Pieter Bautmans, Ivan |
author_facet | Scafoglieri, Aldo Tresignie, Jonathan Provyn, Steven Clarys, Jan Pieter Bautmans, Ivan |
author_sort | Scafoglieri, Aldo |
collection | PubMed |
description | INTRODUCTION: The determination of lipid biomarkers by capillary sampling may be useful in the screening, diagnosis and/or personal management of hyperlipidemia and cardiovascular risk. It remains unclear whether the use of the Accutrend(®) Plus system is appropriate. This study aimed to assess its reproducibility, accuracy and concordance for blood lipid profiling in adults. MATERIALS AND METHODS: Fasting capillary total cholesterol (TC) and triglyceride (TG) concentration on Accutrend(®) Plus were compared with their venous analogues obtained by a laboratory reference method in sixty-one adults (27 men and 34 women, aged 33.0 years). Supplementary capillary sampling was performed at two consecutive days taking into account macro-nutrient intake. RESULTS: The day-to-day reproducibility of the Accutrend(®) Plus system proved to be high for TC (ICC = 0.85, P < 0.001), but moderate for TG (ICC = 0.68, P < 0.001). Strong correlations (r ≥ 0.80, P < 0.001) with the reference method were found for TC and TG. Mean difference (limits of agreement) were: 0.26 mmol/L (−0.95, 1.47) for TC, and −0.16 mmol/L (−1.29, 0.98) for TG. The concordance for subject classification according to the National Cholesterol Education Program (NCEP) guidelines was significant (P < 0.001), with substantial agreement for TC (κ(w) = 0.67), and moderate agreement for TG (κ(w) = 0.50). CONCLUSIONS: Day-to-day reproducibility of the Accutrend(®) Plus device for TC and TG is not optimal and lacks accuracy when compared to the reference laboratory method. The concordance between both methods for classifying subjects according to the NCEP is inadequate. Accutrend(®) Plus device should not be interchangeably used as a substitution for the standard laboratory methods in the diagnosis of hyperlipidemia. |
format | Online Article Text |
id | pubmed-4062330 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Croatian Society of Medical Biochemistry and Laboratory Medicine |
record_format | MEDLINE/PubMed |
spelling | pubmed-40623302014-06-23 Reproducibility, accuracy and concordance of Accutrend(®) Plus for measuring circulating lipid concentration in adults Scafoglieri, Aldo Tresignie, Jonathan Provyn, Steven Clarys, Jan Pieter Bautmans, Ivan Biochem Med (Zagreb) Original Article INTRODUCTION: The determination of lipid biomarkers by capillary sampling may be useful in the screening, diagnosis and/or personal management of hyperlipidemia and cardiovascular risk. It remains unclear whether the use of the Accutrend(®) Plus system is appropriate. This study aimed to assess its reproducibility, accuracy and concordance for blood lipid profiling in adults. MATERIALS AND METHODS: Fasting capillary total cholesterol (TC) and triglyceride (TG) concentration on Accutrend(®) Plus were compared with their venous analogues obtained by a laboratory reference method in sixty-one adults (27 men and 34 women, aged 33.0 years). Supplementary capillary sampling was performed at two consecutive days taking into account macro-nutrient intake. RESULTS: The day-to-day reproducibility of the Accutrend(®) Plus system proved to be high for TC (ICC = 0.85, P < 0.001), but moderate for TG (ICC = 0.68, P < 0.001). Strong correlations (r ≥ 0.80, P < 0.001) with the reference method were found for TC and TG. Mean difference (limits of agreement) were: 0.26 mmol/L (−0.95, 1.47) for TC, and −0.16 mmol/L (−1.29, 0.98) for TG. The concordance for subject classification according to the National Cholesterol Education Program (NCEP) guidelines was significant (P < 0.001), with substantial agreement for TC (κ(w) = 0.67), and moderate agreement for TG (κ(w) = 0.50). CONCLUSIONS: Day-to-day reproducibility of the Accutrend(®) Plus device for TC and TG is not optimal and lacks accuracy when compared to the reference laboratory method. The concordance between both methods for classifying subjects according to the NCEP is inadequate. Accutrend(®) Plus device should not be interchangeably used as a substitution for the standard laboratory methods in the diagnosis of hyperlipidemia. Croatian Society of Medical Biochemistry and Laboratory Medicine 2012-02-15 /pmc/articles/PMC4062330/ /pubmed/22384524 Text en © Copyright by Croatian Society of Medical Biochemistry and Laboratory Medicine. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc-nd/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Scafoglieri, Aldo Tresignie, Jonathan Provyn, Steven Clarys, Jan Pieter Bautmans, Ivan Reproducibility, accuracy and concordance of Accutrend(®) Plus for measuring circulating lipid concentration in adults |
title | Reproducibility, accuracy and concordance of Accutrend(®) Plus for measuring circulating lipid concentration in adults |
title_full | Reproducibility, accuracy and concordance of Accutrend(®) Plus for measuring circulating lipid concentration in adults |
title_fullStr | Reproducibility, accuracy and concordance of Accutrend(®) Plus for measuring circulating lipid concentration in adults |
title_full_unstemmed | Reproducibility, accuracy and concordance of Accutrend(®) Plus for measuring circulating lipid concentration in adults |
title_short | Reproducibility, accuracy and concordance of Accutrend(®) Plus for measuring circulating lipid concentration in adults |
title_sort | reproducibility, accuracy and concordance of accutrend(®) plus for measuring circulating lipid concentration in adults |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4062330/ https://www.ncbi.nlm.nih.gov/pubmed/22384524 |
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