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P2×(7) Receptor in the Kidneys of Diabetic Rats Submitted to Aerobic Training or to N-Acetylcysteine Supplementation
Previous studies in our laboratory showed that N-acetylcysteine supplementation or aerobic training reduced oxidative stress and the progression of diabetic nephropathy in rats. The P2X(7) receptor is up-regulated in pathological conditions, such as diabetes mellitus. This up-regulation is related t...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4062402/ https://www.ncbi.nlm.nih.gov/pubmed/24940871 http://dx.doi.org/10.1371/journal.pone.0097452 |
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author | Rodrigues, Adelson M. Bergamaschi, Cassia T. Fernandes, Maria Jose S. Paredes-Gamero, Edgar J. Curi, Marcus V. Ferreira, Alice T. Araujo, Sergio R. R. Punaro, Giovana R. Maciel, Fabiane R. Nogueira, Guilherme B. Higa, Elisa M. S. |
author_facet | Rodrigues, Adelson M. Bergamaschi, Cassia T. Fernandes, Maria Jose S. Paredes-Gamero, Edgar J. Curi, Marcus V. Ferreira, Alice T. Araujo, Sergio R. R. Punaro, Giovana R. Maciel, Fabiane R. Nogueira, Guilherme B. Higa, Elisa M. S. |
author_sort | Rodrigues, Adelson M. |
collection | PubMed |
description | Previous studies in our laboratory showed that N-acetylcysteine supplementation or aerobic training reduced oxidative stress and the progression of diabetic nephropathy in rats. The P2X(7) receptor is up-regulated in pathological conditions, such as diabetes mellitus. This up-regulation is related to oxidative stress and induces tissue apoptosis or necrosis. The aim of the present study is to assess the role of P2X(7) receptor in the kidneys of diabetic rats submitted to aerobic training or N-acetylcysteine supplementation. Diabetes was induced in male Wistar rats by streptozotocin (60 mg/kg, i.v.) and the training was done on a treadmill; N-acetylcysteine was given in the drinking water (600 mg/L). By confocal microscopy, as compared to control, the kidneys of diabetic rats showed increased P2×7 receptor expression and a higher activation in response to 2′(3′)-O-(4-benzoylbenzoyl) adenosine5'–triphosphate (specific agonist) and adenosine triphosphate (nonspecific agonist) (all p<0.05). All these alterations were reduced in diabetic rats treated with N-acetylcysteine, exercise or both. We also observed measured proteinuria and albuminuria (early marker of diabetic nephropathy) in DM groups. Lipoperoxidation was strongly correlated with P2X(7) receptor expression, which was also correlated to NO(•), thus associating this receptor to oxidative stress and kidney lesion. We suggest that P2X(7) receptor inhibition associated with the maintenance of redox homeostasis could be useful as coadjuvant treatment to delay the progression of diabetic nephropathy. |
format | Online Article Text |
id | pubmed-4062402 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-40624022014-06-24 P2×(7) Receptor in the Kidneys of Diabetic Rats Submitted to Aerobic Training or to N-Acetylcysteine Supplementation Rodrigues, Adelson M. Bergamaschi, Cassia T. Fernandes, Maria Jose S. Paredes-Gamero, Edgar J. Curi, Marcus V. Ferreira, Alice T. Araujo, Sergio R. R. Punaro, Giovana R. Maciel, Fabiane R. Nogueira, Guilherme B. Higa, Elisa M. S. PLoS One Research Article Previous studies in our laboratory showed that N-acetylcysteine supplementation or aerobic training reduced oxidative stress and the progression of diabetic nephropathy in rats. The P2X(7) receptor is up-regulated in pathological conditions, such as diabetes mellitus. This up-regulation is related to oxidative stress and induces tissue apoptosis or necrosis. The aim of the present study is to assess the role of P2X(7) receptor in the kidneys of diabetic rats submitted to aerobic training or N-acetylcysteine supplementation. Diabetes was induced in male Wistar rats by streptozotocin (60 mg/kg, i.v.) and the training was done on a treadmill; N-acetylcysteine was given in the drinking water (600 mg/L). By confocal microscopy, as compared to control, the kidneys of diabetic rats showed increased P2×7 receptor expression and a higher activation in response to 2′(3′)-O-(4-benzoylbenzoyl) adenosine5'–triphosphate (specific agonist) and adenosine triphosphate (nonspecific agonist) (all p<0.05). All these alterations were reduced in diabetic rats treated with N-acetylcysteine, exercise or both. We also observed measured proteinuria and albuminuria (early marker of diabetic nephropathy) in DM groups. Lipoperoxidation was strongly correlated with P2X(7) receptor expression, which was also correlated to NO(•), thus associating this receptor to oxidative stress and kidney lesion. We suggest that P2X(7) receptor inhibition associated with the maintenance of redox homeostasis could be useful as coadjuvant treatment to delay the progression of diabetic nephropathy. Public Library of Science 2014-06-18 /pmc/articles/PMC4062402/ /pubmed/24940871 http://dx.doi.org/10.1371/journal.pone.0097452 Text en © 2014 Rodrigues et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Rodrigues, Adelson M. Bergamaschi, Cassia T. Fernandes, Maria Jose S. Paredes-Gamero, Edgar J. Curi, Marcus V. Ferreira, Alice T. Araujo, Sergio R. R. Punaro, Giovana R. Maciel, Fabiane R. Nogueira, Guilherme B. Higa, Elisa M. S. P2×(7) Receptor in the Kidneys of Diabetic Rats Submitted to Aerobic Training or to N-Acetylcysteine Supplementation |
title | P2×(7) Receptor in the Kidneys of Diabetic Rats Submitted to Aerobic Training or to N-Acetylcysteine Supplementation |
title_full | P2×(7) Receptor in the Kidneys of Diabetic Rats Submitted to Aerobic Training or to N-Acetylcysteine Supplementation |
title_fullStr | P2×(7) Receptor in the Kidneys of Diabetic Rats Submitted to Aerobic Training or to N-Acetylcysteine Supplementation |
title_full_unstemmed | P2×(7) Receptor in the Kidneys of Diabetic Rats Submitted to Aerobic Training or to N-Acetylcysteine Supplementation |
title_short | P2×(7) Receptor in the Kidneys of Diabetic Rats Submitted to Aerobic Training or to N-Acetylcysteine Supplementation |
title_sort | p2×(7) receptor in the kidneys of diabetic rats submitted to aerobic training or to n-acetylcysteine supplementation |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4062402/ https://www.ncbi.nlm.nih.gov/pubmed/24940871 http://dx.doi.org/10.1371/journal.pone.0097452 |
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