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Molecular Hydrogen Attenuates Neuropathic Pain in Mice

Neuropathic pain remains intractable and the development of new therapeutic strategies are urgently required. Accumulating evidence indicates that overproduction of oxidative stress is a key event in the pathogenesis of neuropathic pain. However, repeated intra-peritoneal or intrathecal injections o...

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Autores principales: Kawaguchi, Masanori, Satoh, Yasushi, Otsubo, Yukiko, Kazama, Tomiei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4062523/
https://www.ncbi.nlm.nih.gov/pubmed/24941001
http://dx.doi.org/10.1371/journal.pone.0100352
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author Kawaguchi, Masanori
Satoh, Yasushi
Otsubo, Yukiko
Kazama, Tomiei
author_facet Kawaguchi, Masanori
Satoh, Yasushi
Otsubo, Yukiko
Kazama, Tomiei
author_sort Kawaguchi, Masanori
collection PubMed
description Neuropathic pain remains intractable and the development of new therapeutic strategies are urgently required. Accumulating evidence indicates that overproduction of oxidative stress is a key event in the pathogenesis of neuropathic pain. However, repeated intra-peritoneal or intrathecal injections of antioxidants are unsuitable for continuous use in therapy. Here we show a novel therapeutic method against neuropathic pain: drinking water containing molecular hydrogen (H(2)) as antioxidant. The effect of hydrogen on neuropathic pain was investigated using a partial sciatic nerve ligation model in mice. As indicators of neuropathic pain, temporal aspects of mechanical allodynia and thermal hyperalgesia were analysed for 3 weeks after ligation. Mechanical allodynia and thermal hyperalgesia were measured using the von Frey test and the plantar test, respectively. When mice were allowed to drink water containing hydrogen at a saturated level ad libitum after ligation, both allodynia and hyperalgesia were alleviated. These symptoms were also alleviated when hydrogen was administered only for the induction phase (from day 0 to 4 after ligation). When hydrogen was administered only for the maintenance phase (from day 4 to 21 after ligation), hyperalgesia but not allodynia was alleviated. Immunohistochemical staining for the oxidative stress marker, 4-hydroxy-2-nonenal and 8-hydroxydeoxyguanosine, showed that hydrogen administration suppressed oxidative stress induced by ligation in the spinal cord and the dorsal root ganglion. In conclusion, oral administration of hydrogen water may be useful for alleviating neuropathic pain in a clinical setting.
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spelling pubmed-40625232014-06-24 Molecular Hydrogen Attenuates Neuropathic Pain in Mice Kawaguchi, Masanori Satoh, Yasushi Otsubo, Yukiko Kazama, Tomiei PLoS One Research Article Neuropathic pain remains intractable and the development of new therapeutic strategies are urgently required. Accumulating evidence indicates that overproduction of oxidative stress is a key event in the pathogenesis of neuropathic pain. However, repeated intra-peritoneal or intrathecal injections of antioxidants are unsuitable for continuous use in therapy. Here we show a novel therapeutic method against neuropathic pain: drinking water containing molecular hydrogen (H(2)) as antioxidant. The effect of hydrogen on neuropathic pain was investigated using a partial sciatic nerve ligation model in mice. As indicators of neuropathic pain, temporal aspects of mechanical allodynia and thermal hyperalgesia were analysed for 3 weeks after ligation. Mechanical allodynia and thermal hyperalgesia were measured using the von Frey test and the plantar test, respectively. When mice were allowed to drink water containing hydrogen at a saturated level ad libitum after ligation, both allodynia and hyperalgesia were alleviated. These symptoms were also alleviated when hydrogen was administered only for the induction phase (from day 0 to 4 after ligation). When hydrogen was administered only for the maintenance phase (from day 4 to 21 after ligation), hyperalgesia but not allodynia was alleviated. Immunohistochemical staining for the oxidative stress marker, 4-hydroxy-2-nonenal and 8-hydroxydeoxyguanosine, showed that hydrogen administration suppressed oxidative stress induced by ligation in the spinal cord and the dorsal root ganglion. In conclusion, oral administration of hydrogen water may be useful for alleviating neuropathic pain in a clinical setting. Public Library of Science 2014-06-18 /pmc/articles/PMC4062523/ /pubmed/24941001 http://dx.doi.org/10.1371/journal.pone.0100352 Text en © 2014 Kawaguchi et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Kawaguchi, Masanori
Satoh, Yasushi
Otsubo, Yukiko
Kazama, Tomiei
Molecular Hydrogen Attenuates Neuropathic Pain in Mice
title Molecular Hydrogen Attenuates Neuropathic Pain in Mice
title_full Molecular Hydrogen Attenuates Neuropathic Pain in Mice
title_fullStr Molecular Hydrogen Attenuates Neuropathic Pain in Mice
title_full_unstemmed Molecular Hydrogen Attenuates Neuropathic Pain in Mice
title_short Molecular Hydrogen Attenuates Neuropathic Pain in Mice
title_sort molecular hydrogen attenuates neuropathic pain in mice
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4062523/
https://www.ncbi.nlm.nih.gov/pubmed/24941001
http://dx.doi.org/10.1371/journal.pone.0100352
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