Unusual Multiple Production of N-Acylhomoserine Lactones a by Burkholderia sp. Strain C10B Isolated from Dentine Caries

Bacteria realize the ability to communicate by production of quorum sensing (QS) molecules called autoinducers, which regulate the physiological activities in their ecological niches. The oral cavity could be a potential area for the presence of QS bacteria. In this study, we report the isolation of...

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Autores principales: Goh, Share Yuan, Tan, Wen-Si, Khan, Saad Ahmed, Chew, Hooi Pin, Kasim, Noor Hayaty Abu, Yin, Wai-Fong, Chan, Kok-Gan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4063041/
https://www.ncbi.nlm.nih.gov/pubmed/24854358
http://dx.doi.org/10.3390/s140508940
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author Goh, Share Yuan
Tan, Wen-Si
Khan, Saad Ahmed
Chew, Hooi Pin
Kasim, Noor Hayaty Abu
Yin, Wai-Fong
Chan, Kok-Gan
author_facet Goh, Share Yuan
Tan, Wen-Si
Khan, Saad Ahmed
Chew, Hooi Pin
Kasim, Noor Hayaty Abu
Yin, Wai-Fong
Chan, Kok-Gan
author_sort Goh, Share Yuan
collection PubMed
description Bacteria realize the ability to communicate by production of quorum sensing (QS) molecules called autoinducers, which regulate the physiological activities in their ecological niches. The oral cavity could be a potential area for the presence of QS bacteria. In this study, we report the isolation of a QS bacterial isolate C10B from dentine caries. Preliminary screening using Chromobacterium violaceum CV026 biosensor showed that isolate C10B was able to produce N-acylhomoserine lactones (AHLs). This bacterium was further identified as a member of Burkholderia, an opportunistic pathogen. The isolated Burkholderia sp. was confirmed to produce N-hexanoyl-L-homoserine lactone (C6-HSL), N-octanoyl-L-homoserine lactone (C8-HSL), N-decanoyl-L-homoserine lactone (C10-HSL) and N-dodecanoyl-L-homoserine lactone (C12-HSL).
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spelling pubmed-40630412014-06-19 Unusual Multiple Production of N-Acylhomoserine Lactones a by Burkholderia sp. Strain C10B Isolated from Dentine Caries Goh, Share Yuan Tan, Wen-Si Khan, Saad Ahmed Chew, Hooi Pin Kasim, Noor Hayaty Abu Yin, Wai-Fong Chan, Kok-Gan Sensors (Basel) Article Bacteria realize the ability to communicate by production of quorum sensing (QS) molecules called autoinducers, which regulate the physiological activities in their ecological niches. The oral cavity could be a potential area for the presence of QS bacteria. In this study, we report the isolation of a QS bacterial isolate C10B from dentine caries. Preliminary screening using Chromobacterium violaceum CV026 biosensor showed that isolate C10B was able to produce N-acylhomoserine lactones (AHLs). This bacterium was further identified as a member of Burkholderia, an opportunistic pathogen. The isolated Burkholderia sp. was confirmed to produce N-hexanoyl-L-homoserine lactone (C6-HSL), N-octanoyl-L-homoserine lactone (C8-HSL), N-decanoyl-L-homoserine lactone (C10-HSL) and N-dodecanoyl-L-homoserine lactone (C12-HSL). Molecular Diversity Preservation International (MDPI) 2014-05-21 /pmc/articles/PMC4063041/ /pubmed/24854358 http://dx.doi.org/10.3390/s140508940 Text en © 2014 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Goh, Share Yuan
Tan, Wen-Si
Khan, Saad Ahmed
Chew, Hooi Pin
Kasim, Noor Hayaty Abu
Yin, Wai-Fong
Chan, Kok-Gan
Unusual Multiple Production of N-Acylhomoserine Lactones a by Burkholderia sp. Strain C10B Isolated from Dentine Caries
title Unusual Multiple Production of N-Acylhomoserine Lactones a by Burkholderia sp. Strain C10B Isolated from Dentine Caries
title_full Unusual Multiple Production of N-Acylhomoserine Lactones a by Burkholderia sp. Strain C10B Isolated from Dentine Caries
title_fullStr Unusual Multiple Production of N-Acylhomoserine Lactones a by Burkholderia sp. Strain C10B Isolated from Dentine Caries
title_full_unstemmed Unusual Multiple Production of N-Acylhomoserine Lactones a by Burkholderia sp. Strain C10B Isolated from Dentine Caries
title_short Unusual Multiple Production of N-Acylhomoserine Lactones a by Burkholderia sp. Strain C10B Isolated from Dentine Caries
title_sort unusual multiple production of n-acylhomoserine lactones a by burkholderia sp. strain c10b isolated from dentine caries
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4063041/
https://www.ncbi.nlm.nih.gov/pubmed/24854358
http://dx.doi.org/10.3390/s140508940
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