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Diagnostic validity of the chemiluminescent method compared to polymerase chain reaction for hepatitis B virus detection in the routine clinical diagnostic laboratory

BACKGROUND: Hepatitis B virus (HBV) is the most common significant chronic viral infection world-wide. Hepatitis B surface antigen (HBsAg) has been the principal target for laboratory testing to identify active infection by HBV. We aimed to find out diagnostic validity of the Liaison chemiluminescen...

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Autores principales: Khadem-Ansari, Mohammad-Hassan, Omrani, Mir-Davood, Rasmi, Yousef, Ghavam, Arsalan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4063113/
https://www.ncbi.nlm.nih.gov/pubmed/24949287
http://dx.doi.org/10.4103/2277-9175.133178
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author Khadem-Ansari, Mohammad-Hassan
Omrani, Mir-Davood
Rasmi, Yousef
Ghavam, Arsalan
author_facet Khadem-Ansari, Mohammad-Hassan
Omrani, Mir-Davood
Rasmi, Yousef
Ghavam, Arsalan
author_sort Khadem-Ansari, Mohammad-Hassan
collection PubMed
description BACKGROUND: Hepatitis B virus (HBV) is the most common significant chronic viral infection world-wide. Hepatitis B surface antigen (HBsAg) has been the principal target for laboratory testing to identify active infection by HBV. We aimed to find out diagnostic validity of the Liaison chemiluminescent method compared to the polymerase chain reaction (PCR) method for HBV detection in the routine clinical diagnostic laboratory. MATERIALS AND METHODS: From 350 patients suspicious of having infection with HBV, serum samples were separated and used for testing HBsAg by two methods of Liaison chemiluminescent immunoassay, with HBsAg confirmatory test and PCR method. RESULTS: According to the PCR results as assumed as gold standard method with 100% sensitivity and specificity, detection rate sensitivity of chemiluminescent with confirmatory test was 96% and its specificity was 100%, and for chemiluminescent without confirmatory test sensitivity and specificity were 100% and 70%, respectively. Also for chemiluminescent with confirmatory test, positive predictive value (PPV) was 100% and its negative predictive value (NPV) was 97%, compared to chemiluminescent without confirmatory test with PPV and NPV equal to 71% and 100%, respectively. CONCLUSIONS: It is possible to conclude that in the majority of the HBV cases, the diagnostic value of chemiluminescent method compared to the PCR method is acceptable, except in low indexes positive cases that need further investigation with the PCR method.
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spelling pubmed-40631132014-06-19 Diagnostic validity of the chemiluminescent method compared to polymerase chain reaction for hepatitis B virus detection in the routine clinical diagnostic laboratory Khadem-Ansari, Mohammad-Hassan Omrani, Mir-Davood Rasmi, Yousef Ghavam, Arsalan Adv Biomed Res Original Article BACKGROUND: Hepatitis B virus (HBV) is the most common significant chronic viral infection world-wide. Hepatitis B surface antigen (HBsAg) has been the principal target for laboratory testing to identify active infection by HBV. We aimed to find out diagnostic validity of the Liaison chemiluminescent method compared to the polymerase chain reaction (PCR) method for HBV detection in the routine clinical diagnostic laboratory. MATERIALS AND METHODS: From 350 patients suspicious of having infection with HBV, serum samples were separated and used for testing HBsAg by two methods of Liaison chemiluminescent immunoassay, with HBsAg confirmatory test and PCR method. RESULTS: According to the PCR results as assumed as gold standard method with 100% sensitivity and specificity, detection rate sensitivity of chemiluminescent with confirmatory test was 96% and its specificity was 100%, and for chemiluminescent without confirmatory test sensitivity and specificity were 100% and 70%, respectively. Also for chemiluminescent with confirmatory test, positive predictive value (PPV) was 100% and its negative predictive value (NPV) was 97%, compared to chemiluminescent without confirmatory test with PPV and NPV equal to 71% and 100%, respectively. CONCLUSIONS: It is possible to conclude that in the majority of the HBV cases, the diagnostic value of chemiluminescent method compared to the PCR method is acceptable, except in low indexes positive cases that need further investigation with the PCR method. Medknow Publications & Media Pvt Ltd 2014-05-28 /pmc/articles/PMC4063113/ /pubmed/24949287 http://dx.doi.org/10.4103/2277-9175.133178 Text en Copyright: © 2014 Khadem-Ansari http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Original Article
Khadem-Ansari, Mohammad-Hassan
Omrani, Mir-Davood
Rasmi, Yousef
Ghavam, Arsalan
Diagnostic validity of the chemiluminescent method compared to polymerase chain reaction for hepatitis B virus detection in the routine clinical diagnostic laboratory
title Diagnostic validity of the chemiluminescent method compared to polymerase chain reaction for hepatitis B virus detection in the routine clinical diagnostic laboratory
title_full Diagnostic validity of the chemiluminescent method compared to polymerase chain reaction for hepatitis B virus detection in the routine clinical diagnostic laboratory
title_fullStr Diagnostic validity of the chemiluminescent method compared to polymerase chain reaction for hepatitis B virus detection in the routine clinical diagnostic laboratory
title_full_unstemmed Diagnostic validity of the chemiluminescent method compared to polymerase chain reaction for hepatitis B virus detection in the routine clinical diagnostic laboratory
title_short Diagnostic validity of the chemiluminescent method compared to polymerase chain reaction for hepatitis B virus detection in the routine clinical diagnostic laboratory
title_sort diagnostic validity of the chemiluminescent method compared to polymerase chain reaction for hepatitis b virus detection in the routine clinical diagnostic laboratory
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4063113/
https://www.ncbi.nlm.nih.gov/pubmed/24949287
http://dx.doi.org/10.4103/2277-9175.133178
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