A red-shifted fluorescent substrate for aldehyde dehydrogenase

Selection of cells positive for aldehyde dehydrogenase (ALDH) activity from a green fluorescent background is difficult with existing reagents. Here we report a red-shifted fluorescent substrate for ALDH, AldeRed 588-A, for labeling viable ALDH(pos) cells. We demonstrate that AldeRed 588-A successfu...

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Detalles Bibliográficos
Autores principales: Minn, Il, Wang, Haofan, Mease, Ronnie C., Byun, Youngjoo, Yang, Xing, Wang, Julia, Leach, Steven D., Pomper, Martin G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4063304/
https://www.ncbi.nlm.nih.gov/pubmed/24759454
http://dx.doi.org/10.1038/ncomms4662
Descripción
Sumario:Selection of cells positive for aldehyde dehydrogenase (ALDH) activity from a green fluorescent background is difficult with existing reagents. Here we report a red-shifted fluorescent substrate for ALDH, AldeRed 588-A, for labeling viable ALDH(pos) cells. We demonstrate that AldeRed 588-A successfully isolates ALDH(hi) human hematopoietic stem cells from heterogeneous cord blood mononuclear cells. AldeRed 588-A can be used for multi-color applications to fractionate ALDH(pos) cells in the presence of green fluorophores including the ALDEFLUOR™ reagent and cells expressing eGFP. AldeRed 588-A stains ALDH(pos) murine pancreatic centroacinar and terminal duct cells, as visualized by fluorescent microscopy. AldeRed588-A provides a useful tool to select stem cells or study ALDH within a green fluorescent background.

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