A red-shifted fluorescent substrate for aldehyde dehydrogenase

Selection of cells positive for aldehyde dehydrogenase (ALDH) activity from a green fluorescent background is difficult with existing reagents. Here we report a red-shifted fluorescent substrate for ALDH, AldeRed 588-A, for labeling viable ALDH(pos) cells. We demonstrate that AldeRed 588-A successfu...

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Detalles Bibliográficos
Autores principales: Minn, Il, Wang, Haofan, Mease, Ronnie C., Byun, Youngjoo, Yang, Xing, Wang, Julia, Leach, Steven D., Pomper, Martin G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4063304/
https://www.ncbi.nlm.nih.gov/pubmed/24759454
http://dx.doi.org/10.1038/ncomms4662
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author Minn, Il
Wang, Haofan
Mease, Ronnie C.
Byun, Youngjoo
Yang, Xing
Wang, Julia
Leach, Steven D.
Pomper, Martin G.
author_facet Minn, Il
Wang, Haofan
Mease, Ronnie C.
Byun, Youngjoo
Yang, Xing
Wang, Julia
Leach, Steven D.
Pomper, Martin G.
author_sort Minn, Il
collection PubMed
description Selection of cells positive for aldehyde dehydrogenase (ALDH) activity from a green fluorescent background is difficult with existing reagents. Here we report a red-shifted fluorescent substrate for ALDH, AldeRed 588-A, for labeling viable ALDH(pos) cells. We demonstrate that AldeRed 588-A successfully isolates ALDH(hi) human hematopoietic stem cells from heterogeneous cord blood mononuclear cells. AldeRed 588-A can be used for multi-color applications to fractionate ALDH(pos) cells in the presence of green fluorophores including the ALDEFLUOR™ reagent and cells expressing eGFP. AldeRed 588-A stains ALDH(pos) murine pancreatic centroacinar and terminal duct cells, as visualized by fluorescent microscopy. AldeRed588-A provides a useful tool to select stem cells or study ALDH within a green fluorescent background.
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spelling pubmed-40633042014-10-23 A red-shifted fluorescent substrate for aldehyde dehydrogenase Minn, Il Wang, Haofan Mease, Ronnie C. Byun, Youngjoo Yang, Xing Wang, Julia Leach, Steven D. Pomper, Martin G. Nat Commun Article Selection of cells positive for aldehyde dehydrogenase (ALDH) activity from a green fluorescent background is difficult with existing reagents. Here we report a red-shifted fluorescent substrate for ALDH, AldeRed 588-A, for labeling viable ALDH(pos) cells. We demonstrate that AldeRed 588-A successfully isolates ALDH(hi) human hematopoietic stem cells from heterogeneous cord blood mononuclear cells. AldeRed 588-A can be used for multi-color applications to fractionate ALDH(pos) cells in the presence of green fluorophores including the ALDEFLUOR™ reagent and cells expressing eGFP. AldeRed 588-A stains ALDH(pos) murine pancreatic centroacinar and terminal duct cells, as visualized by fluorescent microscopy. AldeRed588-A provides a useful tool to select stem cells or study ALDH within a green fluorescent background. 2014-04-23 /pmc/articles/PMC4063304/ /pubmed/24759454 http://dx.doi.org/10.1038/ncomms4662 Text en http://www.nature.com/authors/editorial_policies/license.html#terms Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Minn, Il
Wang, Haofan
Mease, Ronnie C.
Byun, Youngjoo
Yang, Xing
Wang, Julia
Leach, Steven D.
Pomper, Martin G.
A red-shifted fluorescent substrate for aldehyde dehydrogenase
title A red-shifted fluorescent substrate for aldehyde dehydrogenase
title_full A red-shifted fluorescent substrate for aldehyde dehydrogenase
title_fullStr A red-shifted fluorescent substrate for aldehyde dehydrogenase
title_full_unstemmed A red-shifted fluorescent substrate for aldehyde dehydrogenase
title_short A red-shifted fluorescent substrate for aldehyde dehydrogenase
title_sort red-shifted fluorescent substrate for aldehyde dehydrogenase
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4063304/
https://www.ncbi.nlm.nih.gov/pubmed/24759454
http://dx.doi.org/10.1038/ncomms4662
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