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Angiopoietin-like 4 promotes melanoma cell invasion and survival through aldolase A

In the present study, the association between angiopoietin-like 4 (ANGPTL4) and aldolase A (ALDOA) in human melanoma cell invasion and survival was investigated. Overexpression and knockdown of ANGPTL4 were respectively performed in WM-115 and WM-266-4 cells. ALDOA expression at both the mRNA and th...

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Autores principales: SUN, YANG, LONG, JIANHONG, ZHOU, YU
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4063564/
https://www.ncbi.nlm.nih.gov/pubmed/24959248
http://dx.doi.org/10.3892/ol.2014.2071
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author SUN, YANG
LONG, JIANHONG
ZHOU, YU
author_facet SUN, YANG
LONG, JIANHONG
ZHOU, YU
author_sort SUN, YANG
collection PubMed
description In the present study, the association between angiopoietin-like 4 (ANGPTL4) and aldolase A (ALDOA) in human melanoma cell invasion and survival was investigated. Overexpression and knockdown of ANGPTL4 were respectively performed in WM-115 and WM-266-4 cells. ALDOA expression at both the mRNA and the protein levels as well as the ALDOA gene promoter activities were increased and decreased in parallel with overexpression and knockdown of ANGPTL4 in the melanoma cells, which was blocked by selective protein kinase C (PKC) inhibitor and restored by PKC agonist, respectively. ANGPTL4 overexpression significantly increased cell invasion and matrix metalloproteinase-2 (MMP-2) expression and decreased cell apoptosis against cisplatin in WM-115 cells, which was reversed by knocking down ALDOA. In WM-266-4 cells, knockdown of ANGPTL4 decreased cell invasion and MMP-2 expression and increased cell apoptosis against cisplatin, which was reversed by overexpression of ALDOA. In conclusion, this study demonstrates that ANGPTL4 upregulates ALDOA expression in human melanoma cells at the ALDOA gene promoter/transcriptional level through a PKC-dependent mechanism, and that ALDOA is a critical mediator of the promoting effect of ANGPTL4 on melanoma cell invasion, likely through upregulating the MMP-2 expression. Additionally, our results suggest that ALDOA plays an important role in ANGPTL4-enhanced melanoma cell survival against apoptotic stress, which implicates ANGPTL4 and ALDOA in the development of melanoma chemoresistance.
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spelling pubmed-40635642014-06-23 Angiopoietin-like 4 promotes melanoma cell invasion and survival through aldolase A SUN, YANG LONG, JIANHONG ZHOU, YU Oncol Lett Articles In the present study, the association between angiopoietin-like 4 (ANGPTL4) and aldolase A (ALDOA) in human melanoma cell invasion and survival was investigated. Overexpression and knockdown of ANGPTL4 were respectively performed in WM-115 and WM-266-4 cells. ALDOA expression at both the mRNA and the protein levels as well as the ALDOA gene promoter activities were increased and decreased in parallel with overexpression and knockdown of ANGPTL4 in the melanoma cells, which was blocked by selective protein kinase C (PKC) inhibitor and restored by PKC agonist, respectively. ANGPTL4 overexpression significantly increased cell invasion and matrix metalloproteinase-2 (MMP-2) expression and decreased cell apoptosis against cisplatin in WM-115 cells, which was reversed by knocking down ALDOA. In WM-266-4 cells, knockdown of ANGPTL4 decreased cell invasion and MMP-2 expression and increased cell apoptosis against cisplatin, which was reversed by overexpression of ALDOA. In conclusion, this study demonstrates that ANGPTL4 upregulates ALDOA expression in human melanoma cells at the ALDOA gene promoter/transcriptional level through a PKC-dependent mechanism, and that ALDOA is a critical mediator of the promoting effect of ANGPTL4 on melanoma cell invasion, likely through upregulating the MMP-2 expression. Additionally, our results suggest that ALDOA plays an important role in ANGPTL4-enhanced melanoma cell survival against apoptotic stress, which implicates ANGPTL4 and ALDOA in the development of melanoma chemoresistance. D.A. Spandidos 2014-07 2014-04-16 /pmc/articles/PMC4063564/ /pubmed/24959248 http://dx.doi.org/10.3892/ol.2014.2071 Text en Copyright © 2014, Spandidos Publications http://creativecommons.org/licenses/by/3.0 This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.
spellingShingle Articles
SUN, YANG
LONG, JIANHONG
ZHOU, YU
Angiopoietin-like 4 promotes melanoma cell invasion and survival through aldolase A
title Angiopoietin-like 4 promotes melanoma cell invasion and survival through aldolase A
title_full Angiopoietin-like 4 promotes melanoma cell invasion and survival through aldolase A
title_fullStr Angiopoietin-like 4 promotes melanoma cell invasion and survival through aldolase A
title_full_unstemmed Angiopoietin-like 4 promotes melanoma cell invasion and survival through aldolase A
title_short Angiopoietin-like 4 promotes melanoma cell invasion and survival through aldolase A
title_sort angiopoietin-like 4 promotes melanoma cell invasion and survival through aldolase a
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4063564/
https://www.ncbi.nlm.nih.gov/pubmed/24959248
http://dx.doi.org/10.3892/ol.2014.2071
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AT zhouyu angiopoietinlike4promotesmelanomacellinvasionandsurvivalthroughaldolasea