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Difference in glycerol levels between leukemia and normal bone marrow stem cells

Aquaglyceroporin 9 (AQP9) is considered to be involved in numerous types of carcinogenic processes, particularly in liver carcinoma. AQP9 expression is significantly decreased in the human hepatocellular carcinoma when compared with the non-tumourigenic liver, which leads to increased resistance to...

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Autores principales: QIN, YING-SONG, BU, DAN-XIA, CUI, YING-YING, ZHANG, XIANG-YU
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4063588/
https://www.ncbi.nlm.nih.gov/pubmed/24959239
http://dx.doi.org/10.3892/ol.2014.2142
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author QIN, YING-SONG
BU, DAN-XIA
CUI, YING-YING
ZHANG, XIANG-YU
author_facet QIN, YING-SONG
BU, DAN-XIA
CUI, YING-YING
ZHANG, XIANG-YU
author_sort QIN, YING-SONG
collection PubMed
description Aquaglyceroporin 9 (AQP9) is considered to be involved in numerous types of carcinogenic processes, particularly in liver carcinoma. AQP9 expression is significantly decreased in the human hepatocellular carcinoma when compared with the non-tumourigenic liver, which leads to increased resistance to apoptosis. In addition, AQP9 is permeable to glycerol and urea. The involvement of AQP9 in leukemia has not been fully delineated. It is proposed that abnormal proliferation of hematopoietic stem cells (HSCs) contributes to leukemia carcinogenesis. Therefore, the present study aimed to investigate the possible roles of AQP9 in HSCs and its effect on the intracellular glycerol content. HSCs and non-HSCs (nHSCs) were isolated via magnetic-activated cell sorting and then subjected to flow cytometry for evaluation of purity. White blood cells (WBCs) were isolated from peripheral blood from healthy volunteers. Furthermore, AQP9 expression was examined at the mRNA and protein levels using western blotting and reverse transcription-polymerase chain reaction (RT-PCR). The glycerol content of HSCs, nHSCs and WBCs was evaluated by ELISA. Finally, in order to observe the morphology of HSCs and nHSCs, a blood smear was conducted and the cells were observed with Wright-Giemsa staining. The results indicated that the glycerol content in the HSCs was markedly greater than that in the nHSCs. AQP9 mRNA and protein expression was not detected in the HSCs and nHSCs, but was identified in the WBCs. Moreover, the HSC morphological characteristics included round or oval cells with round, slightly oval or irregularly shaped nuclei. Additionally, the nuclei occupied almost the entire cell, were located in the middle or were biased toward one side, and were stained light purple or red. Overall, our results indicated that intracellular glycerol is involved in HSC proliferation, despite the fact that glycerol is not mediated by AQP9. Hence, our findings may be useful in further understanding the mechanism of leukemia carcinogenesis, and these data may be valuable in developing future therapeutic strategies.
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spelling pubmed-40635882014-06-23 Difference in glycerol levels between leukemia and normal bone marrow stem cells QIN, YING-SONG BU, DAN-XIA CUI, YING-YING ZHANG, XIANG-YU Oncol Lett Articles Aquaglyceroporin 9 (AQP9) is considered to be involved in numerous types of carcinogenic processes, particularly in liver carcinoma. AQP9 expression is significantly decreased in the human hepatocellular carcinoma when compared with the non-tumourigenic liver, which leads to increased resistance to apoptosis. In addition, AQP9 is permeable to glycerol and urea. The involvement of AQP9 in leukemia has not been fully delineated. It is proposed that abnormal proliferation of hematopoietic stem cells (HSCs) contributes to leukemia carcinogenesis. Therefore, the present study aimed to investigate the possible roles of AQP9 in HSCs and its effect on the intracellular glycerol content. HSCs and non-HSCs (nHSCs) were isolated via magnetic-activated cell sorting and then subjected to flow cytometry for evaluation of purity. White blood cells (WBCs) were isolated from peripheral blood from healthy volunteers. Furthermore, AQP9 expression was examined at the mRNA and protein levels using western blotting and reverse transcription-polymerase chain reaction (RT-PCR). The glycerol content of HSCs, nHSCs and WBCs was evaluated by ELISA. Finally, in order to observe the morphology of HSCs and nHSCs, a blood smear was conducted and the cells were observed with Wright-Giemsa staining. The results indicated that the glycerol content in the HSCs was markedly greater than that in the nHSCs. AQP9 mRNA and protein expression was not detected in the HSCs and nHSCs, but was identified in the WBCs. Moreover, the HSC morphological characteristics included round or oval cells with round, slightly oval or irregularly shaped nuclei. Additionally, the nuclei occupied almost the entire cell, were located in the middle or were biased toward one side, and were stained light purple or red. Overall, our results indicated that intracellular glycerol is involved in HSC proliferation, despite the fact that glycerol is not mediated by AQP9. Hence, our findings may be useful in further understanding the mechanism of leukemia carcinogenesis, and these data may be valuable in developing future therapeutic strategies. D.A. Spandidos 2014-07 2014-05-13 /pmc/articles/PMC4063588/ /pubmed/24959239 http://dx.doi.org/10.3892/ol.2014.2142 Text en Copyright © 2014, Spandidos Publications http://creativecommons.org/licenses/by/3.0 This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.
spellingShingle Articles
QIN, YING-SONG
BU, DAN-XIA
CUI, YING-YING
ZHANG, XIANG-YU
Difference in glycerol levels between leukemia and normal bone marrow stem cells
title Difference in glycerol levels between leukemia and normal bone marrow stem cells
title_full Difference in glycerol levels between leukemia and normal bone marrow stem cells
title_fullStr Difference in glycerol levels between leukemia and normal bone marrow stem cells
title_full_unstemmed Difference in glycerol levels between leukemia and normal bone marrow stem cells
title_short Difference in glycerol levels between leukemia and normal bone marrow stem cells
title_sort difference in glycerol levels between leukemia and normal bone marrow stem cells
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4063588/
https://www.ncbi.nlm.nih.gov/pubmed/24959239
http://dx.doi.org/10.3892/ol.2014.2142
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