Cargando…

Antitumor activity of G-quadruplex-interactive agent TMPyP4 with photodynamic therapy in ovarian carcinoma cells

The aim of the present study was to investigate the potential effects of photodynamic therapy (PDT) mediated by the cationic porphyrin, 5,10,15,20-tetra-(N-methyl-4-pyridyl)porphine (TMPyP(4)), on an ovarian carcinoma cell line and the underlying mechanisms by which TMPyP(4)-PDT exerts its actions....

Descripción completa

Detalles Bibliográficos
Autores principales: LIU, HONGLI, LV, CHANGSHUAI, DING, BAIJUAN, WANG, JIE, LI, SHAN, ZHANG, YOUZHONG
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4063630/
https://www.ncbi.nlm.nih.gov/pubmed/24959286
http://dx.doi.org/10.3892/ol.2014.2125
_version_ 1782321825118683136
author LIU, HONGLI
LV, CHANGSHUAI
DING, BAIJUAN
WANG, JIE
LI, SHAN
ZHANG, YOUZHONG
author_facet LIU, HONGLI
LV, CHANGSHUAI
DING, BAIJUAN
WANG, JIE
LI, SHAN
ZHANG, YOUZHONG
author_sort LIU, HONGLI
collection PubMed
description The aim of the present study was to investigate the potential effects of photodynamic therapy (PDT) mediated by the cationic porphyrin, 5,10,15,20-tetra-(N-methyl-4-pyridyl)porphine (TMPyP(4)), on an ovarian carcinoma cell line and the underlying mechanisms by which TMPyP(4)-PDT exerts its actions. The analysis of cell viability, hematoxylin and eosin staining and flow cytometric apoptosis assays revealed that TMPyP(4)-PDT potently suppressed the growth of the A2780 cells in a laser energy- and dose-dependent manner. Mechanically, it was observed that TMPyP(4)-PDT suppressed the proliferation and motility of the A2780 cells. In addition, the expression levels of minichromosome maintenance protein-2 (MCM2) and carbonic anhydrase IX (CA-IX) were detected by western blot analysis. The results indicated that the TMPyP(4)-PDT-induced apoptosis and antimetastatic activity in the A2780 cells was accompanied by the inhibition of the expression of MCM2 and CA-IX. Therefore, TMPyP(4)-PDT may represent a potential therapeutic method for the treatment of ovarian carcinoma.
format Online
Article
Text
id pubmed-4063630
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher D.A. Spandidos
record_format MEDLINE/PubMed
spelling pubmed-40636302014-06-23 Antitumor activity of G-quadruplex-interactive agent TMPyP4 with photodynamic therapy in ovarian carcinoma cells LIU, HONGLI LV, CHANGSHUAI DING, BAIJUAN WANG, JIE LI, SHAN ZHANG, YOUZHONG Oncol Lett Articles The aim of the present study was to investigate the potential effects of photodynamic therapy (PDT) mediated by the cationic porphyrin, 5,10,15,20-tetra-(N-methyl-4-pyridyl)porphine (TMPyP(4)), on an ovarian carcinoma cell line and the underlying mechanisms by which TMPyP(4)-PDT exerts its actions. The analysis of cell viability, hematoxylin and eosin staining and flow cytometric apoptosis assays revealed that TMPyP(4)-PDT potently suppressed the growth of the A2780 cells in a laser energy- and dose-dependent manner. Mechanically, it was observed that TMPyP(4)-PDT suppressed the proliferation and motility of the A2780 cells. In addition, the expression levels of minichromosome maintenance protein-2 (MCM2) and carbonic anhydrase IX (CA-IX) were detected by western blot analysis. The results indicated that the TMPyP(4)-PDT-induced apoptosis and antimetastatic activity in the A2780 cells was accompanied by the inhibition of the expression of MCM2 and CA-IX. Therefore, TMPyP(4)-PDT may represent a potential therapeutic method for the treatment of ovarian carcinoma. D.A. Spandidos 2014-07 2014-05-08 /pmc/articles/PMC4063630/ /pubmed/24959286 http://dx.doi.org/10.3892/ol.2014.2125 Text en Copyright © 2014, Spandidos Publications http://creativecommons.org/licenses/by/3.0 This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.
spellingShingle Articles
LIU, HONGLI
LV, CHANGSHUAI
DING, BAIJUAN
WANG, JIE
LI, SHAN
ZHANG, YOUZHONG
Antitumor activity of G-quadruplex-interactive agent TMPyP4 with photodynamic therapy in ovarian carcinoma cells
title Antitumor activity of G-quadruplex-interactive agent TMPyP4 with photodynamic therapy in ovarian carcinoma cells
title_full Antitumor activity of G-quadruplex-interactive agent TMPyP4 with photodynamic therapy in ovarian carcinoma cells
title_fullStr Antitumor activity of G-quadruplex-interactive agent TMPyP4 with photodynamic therapy in ovarian carcinoma cells
title_full_unstemmed Antitumor activity of G-quadruplex-interactive agent TMPyP4 with photodynamic therapy in ovarian carcinoma cells
title_short Antitumor activity of G-quadruplex-interactive agent TMPyP4 with photodynamic therapy in ovarian carcinoma cells
title_sort antitumor activity of g-quadruplex-interactive agent tmpyp4 with photodynamic therapy in ovarian carcinoma cells
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4063630/
https://www.ncbi.nlm.nih.gov/pubmed/24959286
http://dx.doi.org/10.3892/ol.2014.2125
work_keys_str_mv AT liuhongli antitumoractivityofgquadruplexinteractiveagenttmpyp4withphotodynamictherapyinovariancarcinomacells
AT lvchangshuai antitumoractivityofgquadruplexinteractiveagenttmpyp4withphotodynamictherapyinovariancarcinomacells
AT dingbaijuan antitumoractivityofgquadruplexinteractiveagenttmpyp4withphotodynamictherapyinovariancarcinomacells
AT wangjie antitumoractivityofgquadruplexinteractiveagenttmpyp4withphotodynamictherapyinovariancarcinomacells
AT lishan antitumoractivityofgquadruplexinteractiveagenttmpyp4withphotodynamictherapyinovariancarcinomacells
AT zhangyouzhong antitumoractivityofgquadruplexinteractiveagenttmpyp4withphotodynamictherapyinovariancarcinomacells