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EphA2 enhances the proliferation and invasion ability of LNCaP prostate cancer cells
EphA2 is persistently overexpressed and functionally changed in numerous human cancers. However, to the best of our knowledge, the role that EphA2 plays in prostate cancer is not entirely clear. To investigate the roles of EphA2 in the development and progression of prostate cancer, the present stud...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4063646/ https://www.ncbi.nlm.nih.gov/pubmed/24959216 http://dx.doi.org/10.3892/ol.2014.2093 |
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author | CHEN, PEIJIE HUANG, YAN ZHANG, BO WANG, QIUQUAN BAI, PEIMING |
author_facet | CHEN, PEIJIE HUANG, YAN ZHANG, BO WANG, QIUQUAN BAI, PEIMING |
author_sort | CHEN, PEIJIE |
collection | PubMed |
description | EphA2 is persistently overexpressed and functionally changed in numerous human cancers. However, to the best of our knowledge, the role that EphA2 plays in prostate cancer is not entirely clear. To investigate the roles of EphA2 in the development and progression of prostate cancer, the present study initially evaluated the roles of the EphA2 protein in LNCaP prostate cancer cells using recombinant plasmid, western blot analysis, flow cytometry, Matrigel invasion chamber and the cell counting kit-8 assay. An immunohistochemistry assay was also conducted to observe the effects of EphA2 in prostate cancer tissues. The results demonstrated that the LNCaP human prostate cancer cells that were transfected with pcDNA3.1(+) plasmid-mediated pcDNA3.1(+)-EphA2, markedly enhanced the cell growth and invasion in vitro. Additionally, EphA2 was overexpressed in prostate cancer specimens and the expression of EphA2 was significantly associated with Gleason grade, total prostate-specific antigen, advanced clinical stage and lymph node metastasis. Collectively, these results demonstrate that EphA2 is involved in malignant cell behavior and is a potential therapeutic target in human prostate cancer. |
format | Online Article Text |
id | pubmed-4063646 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-40636462014-06-23 EphA2 enhances the proliferation and invasion ability of LNCaP prostate cancer cells CHEN, PEIJIE HUANG, YAN ZHANG, BO WANG, QIUQUAN BAI, PEIMING Oncol Lett Articles EphA2 is persistently overexpressed and functionally changed in numerous human cancers. However, to the best of our knowledge, the role that EphA2 plays in prostate cancer is not entirely clear. To investigate the roles of EphA2 in the development and progression of prostate cancer, the present study initially evaluated the roles of the EphA2 protein in LNCaP prostate cancer cells using recombinant plasmid, western blot analysis, flow cytometry, Matrigel invasion chamber and the cell counting kit-8 assay. An immunohistochemistry assay was also conducted to observe the effects of EphA2 in prostate cancer tissues. The results demonstrated that the LNCaP human prostate cancer cells that were transfected with pcDNA3.1(+) plasmid-mediated pcDNA3.1(+)-EphA2, markedly enhanced the cell growth and invasion in vitro. Additionally, EphA2 was overexpressed in prostate cancer specimens and the expression of EphA2 was significantly associated with Gleason grade, total prostate-specific antigen, advanced clinical stage and lymph node metastasis. Collectively, these results demonstrate that EphA2 is involved in malignant cell behavior and is a potential therapeutic target in human prostate cancer. D.A. Spandidos 2014-07 2014-04-25 /pmc/articles/PMC4063646/ /pubmed/24959216 http://dx.doi.org/10.3892/ol.2014.2093 Text en Copyright © 2014, Spandidos Publications http://creativecommons.org/licenses/by/3.0 This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited. |
spellingShingle | Articles CHEN, PEIJIE HUANG, YAN ZHANG, BO WANG, QIUQUAN BAI, PEIMING EphA2 enhances the proliferation and invasion ability of LNCaP prostate cancer cells |
title | EphA2 enhances the proliferation and invasion ability of LNCaP prostate cancer cells |
title_full | EphA2 enhances the proliferation and invasion ability of LNCaP prostate cancer cells |
title_fullStr | EphA2 enhances the proliferation and invasion ability of LNCaP prostate cancer cells |
title_full_unstemmed | EphA2 enhances the proliferation and invasion ability of LNCaP prostate cancer cells |
title_short | EphA2 enhances the proliferation and invasion ability of LNCaP prostate cancer cells |
title_sort | epha2 enhances the proliferation and invasion ability of lncap prostate cancer cells |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4063646/ https://www.ncbi.nlm.nih.gov/pubmed/24959216 http://dx.doi.org/10.3892/ol.2014.2093 |
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