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Assessment of RainDrop BS-seq as a method for large-scale, targeted bisulfite sequencing
We present a systematic assessment of RainDrop BS-seq, a novel method for large-scale, targeted bisulfite sequencing using microdroplet-based PCR amplification coupled with next-generation sequencing. We compared DNA methylation levels at 498 target loci (1001 PCR amplicons) in human whole blood, os...
Autores principales: | Paul, Dirk S, Guilhamon, Paul, Karpathakis, Anna, Butcher, Lee M, Thirlwell, Christina, Feber, Andrew, Beck, Stephan |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Landes Bioscience
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4063826/ https://www.ncbi.nlm.nih.gov/pubmed/24518816 http://dx.doi.org/10.4161/epi.28041 |
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