Cargando…

The Yeast Deletion Collection: A Decade of Functional Genomics

The yeast deletion collections comprise >21,000 mutant strains that carry precise start-to-stop deletions of ∼6000 open reading frames. This collection includes heterozygous and homozygous diploids, and haploids of both MATa and MATα mating types. The yeast deletion collection, or yeast knockout...

Descripción completa

Detalles Bibliográficos
Autores principales: Giaever, Guri, Nislow, Corey
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Genetics Society of America 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4063906/
https://www.ncbi.nlm.nih.gov/pubmed/24939991
http://dx.doi.org/10.1534/genetics.114.161620
_version_ 1782321873307041792
author Giaever, Guri
Nislow, Corey
author_facet Giaever, Guri
Nislow, Corey
author_sort Giaever, Guri
collection PubMed
description The yeast deletion collections comprise >21,000 mutant strains that carry precise start-to-stop deletions of ∼6000 open reading frames. This collection includes heterozygous and homozygous diploids, and haploids of both MATa and MATα mating types. The yeast deletion collection, or yeast knockout (YKO) set, represents the first and only complete, systematically constructed deletion collection available for any organism. Conceived during the Saccharomyces cerevisiae sequencing project, work on the project began in 1998 and was completed in 2002. The YKO strains have been used in numerous laboratories in >1000 genome-wide screens. This landmark genome project has inspired development of numerous genome-wide technologies in organisms from yeast to man. Notable spinoff technologies include synthetic genetic array and HIPHOP chemogenomics. In this retrospective, we briefly describe the yeast deletion project and some of its most noteworthy biological contributions and the impact that these collections have had on the yeast research community and on genomics in general.
format Online
Article
Text
id pubmed-4063906
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher Genetics Society of America
record_format MEDLINE/PubMed
spelling pubmed-40639062014-06-23 The Yeast Deletion Collection: A Decade of Functional Genomics Giaever, Guri Nislow, Corey Genetics YeastBook The yeast deletion collections comprise >21,000 mutant strains that carry precise start-to-stop deletions of ∼6000 open reading frames. This collection includes heterozygous and homozygous diploids, and haploids of both MATa and MATα mating types. The yeast deletion collection, or yeast knockout (YKO) set, represents the first and only complete, systematically constructed deletion collection available for any organism. Conceived during the Saccharomyces cerevisiae sequencing project, work on the project began in 1998 and was completed in 2002. The YKO strains have been used in numerous laboratories in >1000 genome-wide screens. This landmark genome project has inspired development of numerous genome-wide technologies in organisms from yeast to man. Notable spinoff technologies include synthetic genetic array and HIPHOP chemogenomics. In this retrospective, we briefly describe the yeast deletion project and some of its most noteworthy biological contributions and the impact that these collections have had on the yeast research community and on genomics in general. Genetics Society of America 2014-06 2014 /pmc/articles/PMC4063906/ /pubmed/24939991 http://dx.doi.org/10.1534/genetics.114.161620 Text en Copyright © 2014 by the Genetics Society of America Available freely online through the author-supported open access option.
spellingShingle YeastBook
Giaever, Guri
Nislow, Corey
The Yeast Deletion Collection: A Decade of Functional Genomics
title The Yeast Deletion Collection: A Decade of Functional Genomics
title_full The Yeast Deletion Collection: A Decade of Functional Genomics
title_fullStr The Yeast Deletion Collection: A Decade of Functional Genomics
title_full_unstemmed The Yeast Deletion Collection: A Decade of Functional Genomics
title_short The Yeast Deletion Collection: A Decade of Functional Genomics
title_sort yeast deletion collection: a decade of functional genomics
topic YeastBook
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4063906/
https://www.ncbi.nlm.nih.gov/pubmed/24939991
http://dx.doi.org/10.1534/genetics.114.161620
work_keys_str_mv AT giaeverguri theyeastdeletioncollectionadecadeoffunctionalgenomics
AT nislowcorey theyeastdeletioncollectionadecadeoffunctionalgenomics
AT giaeverguri yeastdeletioncollectionadecadeoffunctionalgenomics
AT nislowcorey yeastdeletioncollectionadecadeoffunctionalgenomics