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The Yeast Deletion Collection: A Decade of Functional Genomics
The yeast deletion collections comprise >21,000 mutant strains that carry precise start-to-stop deletions of ∼6000 open reading frames. This collection includes heterozygous and homozygous diploids, and haploids of both MATa and MATα mating types. The yeast deletion collection, or yeast knockout...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Genetics Society of America
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4063906/ https://www.ncbi.nlm.nih.gov/pubmed/24939991 http://dx.doi.org/10.1534/genetics.114.161620 |
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author | Giaever, Guri Nislow, Corey |
author_facet | Giaever, Guri Nislow, Corey |
author_sort | Giaever, Guri |
collection | PubMed |
description | The yeast deletion collections comprise >21,000 mutant strains that carry precise start-to-stop deletions of ∼6000 open reading frames. This collection includes heterozygous and homozygous diploids, and haploids of both MATa and MATα mating types. The yeast deletion collection, or yeast knockout (YKO) set, represents the first and only complete, systematically constructed deletion collection available for any organism. Conceived during the Saccharomyces cerevisiae sequencing project, work on the project began in 1998 and was completed in 2002. The YKO strains have been used in numerous laboratories in >1000 genome-wide screens. This landmark genome project has inspired development of numerous genome-wide technologies in organisms from yeast to man. Notable spinoff technologies include synthetic genetic array and HIPHOP chemogenomics. In this retrospective, we briefly describe the yeast deletion project and some of its most noteworthy biological contributions and the impact that these collections have had on the yeast research community and on genomics in general. |
format | Online Article Text |
id | pubmed-4063906 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Genetics Society of America |
record_format | MEDLINE/PubMed |
spelling | pubmed-40639062014-06-23 The Yeast Deletion Collection: A Decade of Functional Genomics Giaever, Guri Nislow, Corey Genetics YeastBook The yeast deletion collections comprise >21,000 mutant strains that carry precise start-to-stop deletions of ∼6000 open reading frames. This collection includes heterozygous and homozygous diploids, and haploids of both MATa and MATα mating types. The yeast deletion collection, or yeast knockout (YKO) set, represents the first and only complete, systematically constructed deletion collection available for any organism. Conceived during the Saccharomyces cerevisiae sequencing project, work on the project began in 1998 and was completed in 2002. The YKO strains have been used in numerous laboratories in >1000 genome-wide screens. This landmark genome project has inspired development of numerous genome-wide technologies in organisms from yeast to man. Notable spinoff technologies include synthetic genetic array and HIPHOP chemogenomics. In this retrospective, we briefly describe the yeast deletion project and some of its most noteworthy biological contributions and the impact that these collections have had on the yeast research community and on genomics in general. Genetics Society of America 2014-06 2014 /pmc/articles/PMC4063906/ /pubmed/24939991 http://dx.doi.org/10.1534/genetics.114.161620 Text en Copyright © 2014 by the Genetics Society of America Available freely online through the author-supported open access option. |
spellingShingle | YeastBook Giaever, Guri Nislow, Corey The Yeast Deletion Collection: A Decade of Functional Genomics |
title | The Yeast Deletion Collection: A Decade of Functional Genomics |
title_full | The Yeast Deletion Collection: A Decade of Functional Genomics |
title_fullStr | The Yeast Deletion Collection: A Decade of Functional Genomics |
title_full_unstemmed | The Yeast Deletion Collection: A Decade of Functional Genomics |
title_short | The Yeast Deletion Collection: A Decade of Functional Genomics |
title_sort | yeast deletion collection: a decade of functional genomics |
topic | YeastBook |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4063906/ https://www.ncbi.nlm.nih.gov/pubmed/24939991 http://dx.doi.org/10.1534/genetics.114.161620 |
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