Canine Amino Acid Transport System X(c)(–): cDNA Sequence, Distribution and Cystine Transport Activity in Lens Epithelial Cells

The cystine transport activity of a lens epithelial cell line originated from a canine mature cataract was investigated. The distinct cystine transport activity was observed, which was inhibited to 28% by extracellular 1 mM glutamate. The cDNA sequences of canine cysteine/glutamate exchanger (xCT) a...

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Detalles Bibliográficos
Autores principales: MARUO, Takuya, KANEMAKI, Nobuyuki, ONDA, Ken, SATO, Reiichiro, ICHIHARA, Nobuteru, OCHIAI, Hideharu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japanese Society of Veterinary Science 2013
Materias:
Internal Medicine
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4064136/
https://www.ncbi.nlm.nih.gov/pubmed/24366150
http://dx.doi.org/10.1292/jvms.13-0170
Descripción
Sumario:The cystine transport activity of a lens epithelial cell line originated from a canine mature cataract was investigated. The distinct cystine transport activity was observed, which was inhibited to 28% by extracellular 1 mM glutamate. The cDNA sequences of canine cysteine/glutamate exchanger (xCT) and 4F2hc were determined. The predicted amino acid sequences were 527 and 533 amino acid polypeptides, respectively. The amino acid sequences of canine xCT and 4F2hc showed high similarities (>80%) to those of humans. The expression of xCT in lens epithelial cell line was confirmed by western blot analysis. RT-PCR analysis revealed high level expression only in the brain, and it was below the detectable level in other tissues.

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