Canine Amino Acid Transport System X(c)(–): cDNA Sequence, Distribution and Cystine Transport Activity in Lens Epithelial Cells

The cystine transport activity of a lens epithelial cell line originated from a canine mature cataract was investigated. The distinct cystine transport activity was observed, which was inhibited to 28% by extracellular 1 mM glutamate. The cDNA sequences of canine cysteine/glutamate exchanger (xCT) a...

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Autores principales: MARUO, Takuya, KANEMAKI, Nobuyuki, ONDA, Ken, SATO, Reiichiro, ICHIHARA, Nobuteru, OCHIAI, Hideharu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japanese Society of Veterinary Science 2013
Materias:
Internal Medicine
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4064136/
https://www.ncbi.nlm.nih.gov/pubmed/24366150
http://dx.doi.org/10.1292/jvms.13-0170
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author MARUO, Takuya
KANEMAKI, Nobuyuki
ONDA, Ken
SATO, Reiichiro
ICHIHARA, Nobuteru
OCHIAI, Hideharu
author_facet MARUO, Takuya
KANEMAKI, Nobuyuki
ONDA, Ken
SATO, Reiichiro
ICHIHARA, Nobuteru
OCHIAI, Hideharu
author_sort MARUO, Takuya
collection PubMed
description The cystine transport activity of a lens epithelial cell line originated from a canine mature cataract was investigated. The distinct cystine transport activity was observed, which was inhibited to 28% by extracellular 1 mM glutamate. The cDNA sequences of canine cysteine/glutamate exchanger (xCT) and 4F2hc were determined. The predicted amino acid sequences were 527 and 533 amino acid polypeptides, respectively. The amino acid sequences of canine xCT and 4F2hc showed high similarities (>80%) to those of humans. The expression of xCT in lens epithelial cell line was confirmed by western blot analysis. RT-PCR analysis revealed high level expression only in the brain, and it was below the detectable level in other tissues.
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spelling pubmed-40641362014-06-25 Canine Amino Acid Transport System X(c)(–): cDNA Sequence, Distribution and Cystine Transport Activity in Lens Epithelial Cells MARUO, Takuya KANEMAKI, Nobuyuki ONDA, Ken SATO, Reiichiro ICHIHARA, Nobuteru OCHIAI, Hideharu J Vet Med Sci Internal Medicine The cystine transport activity of a lens epithelial cell line originated from a canine mature cataract was investigated. The distinct cystine transport activity was observed, which was inhibited to 28% by extracellular 1 mM glutamate. The cDNA sequences of canine cysteine/glutamate exchanger (xCT) and 4F2hc were determined. The predicted amino acid sequences were 527 and 533 amino acid polypeptides, respectively. The amino acid sequences of canine xCT and 4F2hc showed high similarities (>80%) to those of humans. The expression of xCT in lens epithelial cell line was confirmed by western blot analysis. RT-PCR analysis revealed high level expression only in the brain, and it was below the detectable level in other tissues. The Japanese Society of Veterinary Science 2013-12-20 2014-04 /pmc/articles/PMC4064136/ /pubmed/24366150 http://dx.doi.org/10.1292/jvms.13-0170 Text en ©2014 The Japanese Society of Veterinary Science http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License.
spellingShingle Internal Medicine
MARUO, Takuya
KANEMAKI, Nobuyuki
ONDA, Ken
SATO, Reiichiro
ICHIHARA, Nobuteru
OCHIAI, Hideharu
Canine Amino Acid Transport System X(c)(–): cDNA Sequence, Distribution and Cystine Transport Activity in Lens Epithelial Cells
title Canine Amino Acid Transport System X(c)(–): cDNA Sequence, Distribution and Cystine Transport Activity in Lens Epithelial Cells
title_full Canine Amino Acid Transport System X(c)(–): cDNA Sequence, Distribution and Cystine Transport Activity in Lens Epithelial Cells
title_fullStr Canine Amino Acid Transport System X(c)(–): cDNA Sequence, Distribution and Cystine Transport Activity in Lens Epithelial Cells
title_full_unstemmed Canine Amino Acid Transport System X(c)(–): cDNA Sequence, Distribution and Cystine Transport Activity in Lens Epithelial Cells
title_short Canine Amino Acid Transport System X(c)(–): cDNA Sequence, Distribution and Cystine Transport Activity in Lens Epithelial Cells
title_sort canine amino acid transport system x(c)(–): cdna sequence, distribution and cystine transport activity in lens epithelial cells
topic Internal Medicine
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4064136/
https://www.ncbi.nlm.nih.gov/pubmed/24366150
http://dx.doi.org/10.1292/jvms.13-0170
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