Effects of Cryopreservation on the Cell Viability, Proliferative Capacity and Neuronal Differentiation Potential of Canine Bone Marrow Stromal Cells

We investigated the cell viability, proliferative capacity and neuronal differentiation potential of canine bone marrow stromal cells (BMSCs) after cryopreservation. BMSCs were cryopreserved using cryoprotectant solutions with 10% DMSO and 10% FBS (DF group) or without DMSO and FBS (DF-free group);...

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Autores principales: EDAMURA, Kazuya, NAKANO, Rei, FUJIMOTO, Kyohei, TESHIMA, Kenji, ASANO, Kazushi, TANAKA, Shigeo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japanese Society of Veterinary Science 2013
Materias:
Surgery
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4064145/
https://www.ncbi.nlm.nih.gov/pubmed/24334862
http://dx.doi.org/10.1292/jvms.13-0296
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author EDAMURA, Kazuya
NAKANO, Rei
FUJIMOTO, Kyohei
TESHIMA, Kenji
ASANO, Kazushi
TANAKA, Shigeo
author_facet EDAMURA, Kazuya
NAKANO, Rei
FUJIMOTO, Kyohei
TESHIMA, Kenji
ASANO, Kazushi
TANAKA, Shigeo
author_sort EDAMURA, Kazuya
collection PubMed
description We investigated the cell viability, proliferative capacity and neuronal differentiation potential of canine bone marrow stromal cells (BMSCs) after cryopreservation. BMSCs were cryopreserved using cryoprotectant solutions with 10% DMSO and 10% FBS (DF group) or without DMSO and FBS (DF-free group); fresh BMSCs were used as a control. The cell viability and proliferative capacity of BMSCs were similar in the DF-free and control groups, while those in the DF group were lower. In all groups, BMSCs differentiated into neuron-like cells that stained positive against neuron markers, and the mRNA expression levels of neuron markers increased after neuronal induction. In conclusion, cryopreservation with DF-free cryoprotectant solution did not diminish the cell viability, proliferative capacity or neuronal differentiation potential of canine BMSCs.
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spelling pubmed-40641452014-06-25 Effects of Cryopreservation on the Cell Viability, Proliferative Capacity and Neuronal Differentiation Potential of Canine Bone Marrow Stromal Cells EDAMURA, Kazuya NAKANO, Rei FUJIMOTO, Kyohei TESHIMA, Kenji ASANO, Kazushi TANAKA, Shigeo J Vet Med Sci Surgery We investigated the cell viability, proliferative capacity and neuronal differentiation potential of canine bone marrow stromal cells (BMSCs) after cryopreservation. BMSCs were cryopreserved using cryoprotectant solutions with 10% DMSO and 10% FBS (DF group) or without DMSO and FBS (DF-free group); fresh BMSCs were used as a control. The cell viability and proliferative capacity of BMSCs were similar in the DF-free and control groups, while those in the DF group were lower. In all groups, BMSCs differentiated into neuron-like cells that stained positive against neuron markers, and the mRNA expression levels of neuron markers increased after neuronal induction. In conclusion, cryopreservation with DF-free cryoprotectant solution did not diminish the cell viability, proliferative capacity or neuronal differentiation potential of canine BMSCs. The Japanese Society of Veterinary Science 2013-12-13 2014-04 /pmc/articles/PMC4064145/ /pubmed/24334862 http://dx.doi.org/10.1292/jvms.13-0296 Text en ©2014 The Japanese Society of Veterinary Science http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License.
spellingShingle Surgery
EDAMURA, Kazuya
NAKANO, Rei
FUJIMOTO, Kyohei
TESHIMA, Kenji
ASANO, Kazushi
TANAKA, Shigeo
Effects of Cryopreservation on the Cell Viability, Proliferative Capacity and Neuronal Differentiation Potential of Canine Bone Marrow Stromal Cells
title Effects of Cryopreservation on the Cell Viability, Proliferative Capacity and Neuronal Differentiation Potential of Canine Bone Marrow Stromal Cells
title_full Effects of Cryopreservation on the Cell Viability, Proliferative Capacity and Neuronal Differentiation Potential of Canine Bone Marrow Stromal Cells
title_fullStr Effects of Cryopreservation on the Cell Viability, Proliferative Capacity and Neuronal Differentiation Potential of Canine Bone Marrow Stromal Cells
title_full_unstemmed Effects of Cryopreservation on the Cell Viability, Proliferative Capacity and Neuronal Differentiation Potential of Canine Bone Marrow Stromal Cells
title_short Effects of Cryopreservation on the Cell Viability, Proliferative Capacity and Neuronal Differentiation Potential of Canine Bone Marrow Stromal Cells
title_sort effects of cryopreservation on the cell viability, proliferative capacity and neuronal differentiation potential of canine bone marrow stromal cells
topic Surgery
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4064145/
https://www.ncbi.nlm.nih.gov/pubmed/24334862
http://dx.doi.org/10.1292/jvms.13-0296
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