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The selective biotin tagging and thermolysin proteolysis of chloroplast outer envelope proteins reveals information on protein topology and association into complexes
The understanding of chloroplast function requires the precise localization of proteins in each of its sub-compartments. High-sensitivity mass spectrometry has allowed the inventory of proteins in thylakoid, stroma, and envelope fractions. Concerning membrane association, proteins can be either inte...
Autores principales: | Hardré, Hélène, Kuhn, Lauriane, Albrieux, Catherine, Jouhet, Juliette, Michaud, Morgane, Seigneurin-Berny, Daphné, Falconet, Denis, Block, Maryse A., Maréchal, Eric |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4064156/ https://www.ncbi.nlm.nih.gov/pubmed/24999344 http://dx.doi.org/10.3389/fpls.2014.00203 |
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