Rapid prefractionation of complex protein lysates with centrifugal membrane adsorber units improves the resolving power of 2D-PAGE-based proteome analysis

BACKGROUND: Two-dimensional gel electrophoresis (2D-PAGE) has proven over the years to be a reliable and efficient method for separation of hundreds of proteins based on charge and mass. Nevertheless, the complexity of even the simplest proteomes limits the resolving power of 2D-PAGE. This limitatio...

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Detalles Bibliográficos
Autores principales: Doud, Mary Kathryn, Schmidt, Michael W, Hines, David, Naumann, Claudia, Kocourek, Andreas, Kashani-Poor, Noushin, Zeidler, Robert, Wolf, Dieter A
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2004
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC406497/
https://www.ncbi.nlm.nih.gov/pubmed/15109393
http://dx.doi.org/10.1186/1471-2164-5-25

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