Cargando…

iTRAQ-Based Proteomics Reveals Novel Members Involved in Pathogen Challenge in Sea Cucumber Apostichopus japonicus

Skin ulceration syndrome (SUS) is considered to be a major constraint for the stable development of Apostichopus japonicus culture industries. In this study, we investigated protein changes in the coelomocytes of A. japonicus challenged by Vibrio splendidus using isobaric tags for relative and absol...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhang, Pengjuan, Li, Chenghua, Zhang, Peng, Jin, Chunhua, Pan, Daodong, Bao, Yongbo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4065040/
https://www.ncbi.nlm.nih.gov/pubmed/24949634
http://dx.doi.org/10.1371/journal.pone.0100492
_version_ 1782322017427521536
author Zhang, Pengjuan
Li, Chenghua
Zhang, Peng
Jin, Chunhua
Pan, Daodong
Bao, Yongbo
author_facet Zhang, Pengjuan
Li, Chenghua
Zhang, Peng
Jin, Chunhua
Pan, Daodong
Bao, Yongbo
author_sort Zhang, Pengjuan
collection PubMed
description Skin ulceration syndrome (SUS) is considered to be a major constraint for the stable development of Apostichopus japonicus culture industries. In this study, we investigated protein changes in the coelomocytes of A. japonicus challenged by Vibrio splendidus using isobaric tags for relative and absolute quantification (iTRAQ) over a 96 h time course. Consequently, 228 differentially expressed proteins were identified in two iTRAQs. A comparison of the protein expression profiles among different time points detected 125 proteins primarily involved in response to endogenous stimuli at 24 h. At 48 h, the number of differentially expressed proteins decreased to 67, with their primary function being oxidation reduction. At the end of pathogen infection, proteins responsive to amino acid stimuli and some metabolic processes were classified as the predominant group. Fifteen proteins were differentially expressed at all time points, among which eight proteins related to pathologies in higher animals were shown to be down-regulated after V. splendidus infection: paxillin, fascin-2, aggrecan, ololfactomedin-1, nesprin-3, a disintegrin-like and metallopeptidase with thrombospondin type 1 motif (Adamts7), C-type lectin domain family 4 (Clec4g) and n-myc downstream regulated gene 1 (Ndrg1). To gain more insight into two SUS-related miRNA (miR-31 and miR-2008) targets at the protein level, all 129 down-regulated proteins were further analyzed in combination with RNA-seq. Twelve and eight proteins were identified as putative targets for miR-31 and miR-2008, respectively, in which six proteins (5 for miR-31 and 1 for miR-2008) displayed higher possibilities to be regulated at the level of translation. Overall, the present work enhances our understanding of the process of V. splendidus-challenged sea cucumber and provides a new method for screening miRNAs targets at the translation level.
format Online
Article
Text
id pubmed-4065040
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-40650402014-06-25 iTRAQ-Based Proteomics Reveals Novel Members Involved in Pathogen Challenge in Sea Cucumber Apostichopus japonicus Zhang, Pengjuan Li, Chenghua Zhang, Peng Jin, Chunhua Pan, Daodong Bao, Yongbo PLoS One Research Article Skin ulceration syndrome (SUS) is considered to be a major constraint for the stable development of Apostichopus japonicus culture industries. In this study, we investigated protein changes in the coelomocytes of A. japonicus challenged by Vibrio splendidus using isobaric tags for relative and absolute quantification (iTRAQ) over a 96 h time course. Consequently, 228 differentially expressed proteins were identified in two iTRAQs. A comparison of the protein expression profiles among different time points detected 125 proteins primarily involved in response to endogenous stimuli at 24 h. At 48 h, the number of differentially expressed proteins decreased to 67, with their primary function being oxidation reduction. At the end of pathogen infection, proteins responsive to amino acid stimuli and some metabolic processes were classified as the predominant group. Fifteen proteins were differentially expressed at all time points, among which eight proteins related to pathologies in higher animals were shown to be down-regulated after V. splendidus infection: paxillin, fascin-2, aggrecan, ololfactomedin-1, nesprin-3, a disintegrin-like and metallopeptidase with thrombospondin type 1 motif (Adamts7), C-type lectin domain family 4 (Clec4g) and n-myc downstream regulated gene 1 (Ndrg1). To gain more insight into two SUS-related miRNA (miR-31 and miR-2008) targets at the protein level, all 129 down-regulated proteins were further analyzed in combination with RNA-seq. Twelve and eight proteins were identified as putative targets for miR-31 and miR-2008, respectively, in which six proteins (5 for miR-31 and 1 for miR-2008) displayed higher possibilities to be regulated at the level of translation. Overall, the present work enhances our understanding of the process of V. splendidus-challenged sea cucumber and provides a new method for screening miRNAs targets at the translation level. Public Library of Science 2014-06-20 /pmc/articles/PMC4065040/ /pubmed/24949634 http://dx.doi.org/10.1371/journal.pone.0100492 Text en © 2014 Zhang et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Zhang, Pengjuan
Li, Chenghua
Zhang, Peng
Jin, Chunhua
Pan, Daodong
Bao, Yongbo
iTRAQ-Based Proteomics Reveals Novel Members Involved in Pathogen Challenge in Sea Cucumber Apostichopus japonicus
title iTRAQ-Based Proteomics Reveals Novel Members Involved in Pathogen Challenge in Sea Cucumber Apostichopus japonicus
title_full iTRAQ-Based Proteomics Reveals Novel Members Involved in Pathogen Challenge in Sea Cucumber Apostichopus japonicus
title_fullStr iTRAQ-Based Proteomics Reveals Novel Members Involved in Pathogen Challenge in Sea Cucumber Apostichopus japonicus
title_full_unstemmed iTRAQ-Based Proteomics Reveals Novel Members Involved in Pathogen Challenge in Sea Cucumber Apostichopus japonicus
title_short iTRAQ-Based Proteomics Reveals Novel Members Involved in Pathogen Challenge in Sea Cucumber Apostichopus japonicus
title_sort itraq-based proteomics reveals novel members involved in pathogen challenge in sea cucumber apostichopus japonicus
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4065040/
https://www.ncbi.nlm.nih.gov/pubmed/24949634
http://dx.doi.org/10.1371/journal.pone.0100492
work_keys_str_mv AT zhangpengjuan itraqbasedproteomicsrevealsnovelmembersinvolvedinpathogenchallengeinseacucumberapostichopusjaponicus
AT lichenghua itraqbasedproteomicsrevealsnovelmembersinvolvedinpathogenchallengeinseacucumberapostichopusjaponicus
AT zhangpeng itraqbasedproteomicsrevealsnovelmembersinvolvedinpathogenchallengeinseacucumberapostichopusjaponicus
AT jinchunhua itraqbasedproteomicsrevealsnovelmembersinvolvedinpathogenchallengeinseacucumberapostichopusjaponicus
AT pandaodong itraqbasedproteomicsrevealsnovelmembersinvolvedinpathogenchallengeinseacucumberapostichopusjaponicus
AT baoyongbo itraqbasedproteomicsrevealsnovelmembersinvolvedinpathogenchallengeinseacucumberapostichopusjaponicus