New construction of an animal model for the orthotopic transplantation of an ovarian tumor

A new technique has successfully established the non-obese diabetic/severely combined immunodeficiency (NOD/SCID) mouse model of ovarian cancer. Under 4% chloral hydrate (0.1 mL/g dose) anesthesia, female mice were inoculated with tumor-cell suspension. The expression rate of OVCAR3 to CA125 was ass...

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Autores principales: Zhang, Hui, Gao, Xinping, Yang, Yongan, Wang, Weiming, Liu, Jin, Liang, Yijuan, Wu, Hongli, Qin, Jinjin, Pan, Kun, Wang, Yifeng, Shi, Junrong, Ma, Youju
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Brief Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4065080/
https://www.ncbi.nlm.nih.gov/pubmed/24955132
http://dx.doi.org/10.1186/1757-2215-7-64
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author Zhang, Hui
Gao, Xinping
Yang, Yongan
Wang, Weiming
Liu, Jin
Liang, Yijuan
Wu, Hongli
Qin, Jinjin
Pan, Kun
Wang, Yifeng
Shi, Junrong
Ma, Youju
author_facet Zhang, Hui
Gao, Xinping
Yang, Yongan
Wang, Weiming
Liu, Jin
Liang, Yijuan
Wu, Hongli
Qin, Jinjin
Pan, Kun
Wang, Yifeng
Shi, Junrong
Ma, Youju
author_sort Zhang, Hui
collection PubMed
description A new technique has successfully established the non-obese diabetic/severely combined immunodeficiency (NOD/SCID) mouse model of ovarian cancer. Under 4% chloral hydrate (0.1 mL/g dose) anesthesia, female mice were inoculated with tumor-cell suspension. The expression rate of OVCAR3 to CA125 was assessed using flow cytometry. The inoculated site was hand palpated and the signs and symptoms related to tumor growth were observed with the naked eye. The allophycocyanin (APC) indirectly labeled mouse-antihuman CA125 and fluorescein isothiocyanate (FITC)-labeled anti-mouse MHC Class I molecule (H-2K(d)/H-2D(d)) were observed using a confocal laser scanning microscope. The animal model of ovarian cancer constructed using this method can more directly reflect the characteristics of cancer cells. It provides reliable experimental results and presents a technical platform for the research of ovarian cancer stem cells.
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spelling pubmed-40650802014-06-21 New construction of an animal model for the orthotopic transplantation of an ovarian tumor Zhang, Hui Gao, Xinping Yang, Yongan Wang, Weiming Liu, Jin Liang, Yijuan Wu, Hongli Qin, Jinjin Pan, Kun Wang, Yifeng Shi, Junrong Ma, Youju J Ovarian Res Brief Communication A new technique has successfully established the non-obese diabetic/severely combined immunodeficiency (NOD/SCID) mouse model of ovarian cancer. Under 4% chloral hydrate (0.1 mL/g dose) anesthesia, female mice were inoculated with tumor-cell suspension. The expression rate of OVCAR3 to CA125 was assessed using flow cytometry. The inoculated site was hand palpated and the signs and symptoms related to tumor growth were observed with the naked eye. The allophycocyanin (APC) indirectly labeled mouse-antihuman CA125 and fluorescein isothiocyanate (FITC)-labeled anti-mouse MHC Class I molecule (H-2K(d)/H-2D(d)) were observed using a confocal laser scanning microscope. The animal model of ovarian cancer constructed using this method can more directly reflect the characteristics of cancer cells. It provides reliable experimental results and presents a technical platform for the research of ovarian cancer stem cells. BioMed Central 2014-06-11 /pmc/articles/PMC4065080/ /pubmed/24955132 http://dx.doi.org/10.1186/1757-2215-7-64 Text en Copyright © 2014 Zhang et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Brief Communication
Zhang, Hui
Gao, Xinping
Yang, Yongan
Wang, Weiming
Liu, Jin
Liang, Yijuan
Wu, Hongli
Qin, Jinjin
Pan, Kun
Wang, Yifeng
Shi, Junrong
Ma, Youju
New construction of an animal model for the orthotopic transplantation of an ovarian tumor
title New construction of an animal model for the orthotopic transplantation of an ovarian tumor
title_full New construction of an animal model for the orthotopic transplantation of an ovarian tumor
title_fullStr New construction of an animal model for the orthotopic transplantation of an ovarian tumor
title_full_unstemmed New construction of an animal model for the orthotopic transplantation of an ovarian tumor
title_short New construction of an animal model for the orthotopic transplantation of an ovarian tumor
title_sort new construction of an animal model for the orthotopic transplantation of an ovarian tumor
topic Brief Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4065080/
https://www.ncbi.nlm.nih.gov/pubmed/24955132
http://dx.doi.org/10.1186/1757-2215-7-64
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