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Collecting, archiving and processing DNA from wildlife samples using FTA(® )databasing paper

BACKGROUND: Methods involving the analysis of nucleic acids have become widespread in the fields of traditional biology and ecology, however the storage and transport of samples collected in the field to the laboratory in such a manner to allow purification of intact nucleic acids can prove problema...

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Detalles Bibliográficos
Autores principales: Smith, LM, Burgoyne, LA
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2004
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC406513/
https://www.ncbi.nlm.nih.gov/pubmed/15072582
http://dx.doi.org/10.1186/1472-6785-4-4
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author Smith, LM
Burgoyne, LA
author_facet Smith, LM
Burgoyne, LA
author_sort Smith, LM
collection PubMed
description BACKGROUND: Methods involving the analysis of nucleic acids have become widespread in the fields of traditional biology and ecology, however the storage and transport of samples collected in the field to the laboratory in such a manner to allow purification of intact nucleic acids can prove problematical. RESULTS: FTA(® )databasing paper is widely used in human forensic analysis for the storage of biological samples and for purification of nucleic acids. The possible uses of FTA(® )databasing paper in the purification of DNA from samples of wildlife origin were examined, with particular reference to problems expected due to the nature of samples of wildlife origin. The processing of blood and tissue samples, the possibility of excess DNA in blood samples due to nucleated erythrocytes, and the analysis of degraded samples were all examined, as was the question of long term storage of blood samples on FTA(® )paper. Examples of the end use of the purified DNA are given for all protocols and the rationale behind the processing procedures is also explained to allow the end user to adjust the protocols as required. CONCLUSIONS: FTA(® )paper is eminently suitable for collection of, and purification of nucleic acids from, biological samples from a wide range of wildlife species. This technology makes the collection and storage of such samples much simpler.
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spelling pubmed-4065132004-05-13 Collecting, archiving and processing DNA from wildlife samples using FTA(® )databasing paper Smith, LM Burgoyne, LA BMC Ecol Methodology Article BACKGROUND: Methods involving the analysis of nucleic acids have become widespread in the fields of traditional biology and ecology, however the storage and transport of samples collected in the field to the laboratory in such a manner to allow purification of intact nucleic acids can prove problematical. RESULTS: FTA(® )databasing paper is widely used in human forensic analysis for the storage of biological samples and for purification of nucleic acids. The possible uses of FTA(® )databasing paper in the purification of DNA from samples of wildlife origin were examined, with particular reference to problems expected due to the nature of samples of wildlife origin. The processing of blood and tissue samples, the possibility of excess DNA in blood samples due to nucleated erythrocytes, and the analysis of degraded samples were all examined, as was the question of long term storage of blood samples on FTA(® )paper. Examples of the end use of the purified DNA are given for all protocols and the rationale behind the processing procedures is also explained to allow the end user to adjust the protocols as required. CONCLUSIONS: FTA(® )paper is eminently suitable for collection of, and purification of nucleic acids from, biological samples from a wide range of wildlife species. This technology makes the collection and storage of such samples much simpler. BioMed Central 2004-04-08 /pmc/articles/PMC406513/ /pubmed/15072582 http://dx.doi.org/10.1186/1472-6785-4-4 Text en Copyright © 2004 Smith and Burgoyne; licensee BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL.
spellingShingle Methodology Article
Smith, LM
Burgoyne, LA
Collecting, archiving and processing DNA from wildlife samples using FTA(® )databasing paper
title Collecting, archiving and processing DNA from wildlife samples using FTA(® )databasing paper
title_full Collecting, archiving and processing DNA from wildlife samples using FTA(® )databasing paper
title_fullStr Collecting, archiving and processing DNA from wildlife samples using FTA(® )databasing paper
title_full_unstemmed Collecting, archiving and processing DNA from wildlife samples using FTA(® )databasing paper
title_short Collecting, archiving and processing DNA from wildlife samples using FTA(® )databasing paper
title_sort collecting, archiving and processing dna from wildlife samples using fta(® )databasing paper
topic Methodology Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC406513/
https://www.ncbi.nlm.nih.gov/pubmed/15072582
http://dx.doi.org/10.1186/1472-6785-4-4
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