Validation of DNA methylation profiling in formalin-fixed paraffin-embedded samples using the Infinium HumanMethylation450 Microarray

A formalin-fixed paraffin-embedded (FFPE) sample usually yields highly degraded DNA, which limits the use of techniques requiring high-quality DNA, such as Infinium Methylation microarrays. To overcome this restriction, we have applied an FFPE restoration procedure consisting of DNA repair and ligat...

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Autores principales: Moran, Sebastián, Vizoso, Miguel, Martinez-Cardús, Anna, Gomez, Antonio, Matías-Guiu, Xavier, Chiavenna, Sebastián M, Fernandez, Andrés G, Esteller, Manel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Landes Bioscience 2014
Materias:
Brief Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4065180/
https://www.ncbi.nlm.nih.gov/pubmed/24732293
http://dx.doi.org/10.4161/epi.28790
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author Moran, Sebastián
Vizoso, Miguel
Martinez-Cardús, Anna
Gomez, Antonio
Matías-Guiu, Xavier
Chiavenna, Sebastián M
Fernandez, Andrés G
Esteller, Manel
author_facet Moran, Sebastián
Vizoso, Miguel
Martinez-Cardús, Anna
Gomez, Antonio
Matías-Guiu, Xavier
Chiavenna, Sebastián M
Fernandez, Andrés G
Esteller, Manel
author_sort Moran, Sebastián
collection PubMed
description A formalin-fixed paraffin-embedded (FFPE) sample usually yields highly degraded DNA, which limits the use of techniques requiring high-quality DNA, such as Infinium Methylation microarrays. To overcome this restriction, we have applied an FFPE restoration procedure consisting of DNA repair and ligation processes in a set of paired fresh-frozen (FF) and FFPE samples. We validated the FFPE results in comparison with matched FF samples, enabling us to use FFPE samples on the Infinium HumanMethylation450 Methylation array.
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spelling pubmed-40651802015-06-01 Validation of DNA methylation profiling in formalin-fixed paraffin-embedded samples using the Infinium HumanMethylation450 Microarray Moran, Sebastián Vizoso, Miguel Martinez-Cardús, Anna Gomez, Antonio Matías-Guiu, Xavier Chiavenna, Sebastián M Fernandez, Andrés G Esteller, Manel Epigenetics Brief Report A formalin-fixed paraffin-embedded (FFPE) sample usually yields highly degraded DNA, which limits the use of techniques requiring high-quality DNA, such as Infinium Methylation microarrays. To overcome this restriction, we have applied an FFPE restoration procedure consisting of DNA repair and ligation processes in a set of paired fresh-frozen (FF) and FFPE samples. We validated the FFPE results in comparison with matched FF samples, enabling us to use FFPE samples on the Infinium HumanMethylation450 Methylation array. Landes Bioscience 2014-06-01 2014-04-14 /pmc/articles/PMC4065180/ /pubmed/24732293 http://dx.doi.org/10.4161/epi.28790 Text en Copyright © 2014 Landes Bioscience http://creativecommons.org/licenses/by-nc/3.0/ This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.
spellingShingle Brief Report
Moran, Sebastián
Vizoso, Miguel
Martinez-Cardús, Anna
Gomez, Antonio
Matías-Guiu, Xavier
Chiavenna, Sebastián M
Fernandez, Andrés G
Esteller, Manel
Validation of DNA methylation profiling in formalin-fixed paraffin-embedded samples using the Infinium HumanMethylation450 Microarray
title Validation of DNA methylation profiling in formalin-fixed paraffin-embedded samples using the Infinium HumanMethylation450 Microarray
title_full Validation of DNA methylation profiling in formalin-fixed paraffin-embedded samples using the Infinium HumanMethylation450 Microarray
title_fullStr Validation of DNA methylation profiling in formalin-fixed paraffin-embedded samples using the Infinium HumanMethylation450 Microarray
title_full_unstemmed Validation of DNA methylation profiling in formalin-fixed paraffin-embedded samples using the Infinium HumanMethylation450 Microarray
title_short Validation of DNA methylation profiling in formalin-fixed paraffin-embedded samples using the Infinium HumanMethylation450 Microarray
title_sort validation of dna methylation profiling in formalin-fixed paraffin-embedded samples using the infinium humanmethylation450 microarray
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4065180/
https://www.ncbi.nlm.nih.gov/pubmed/24732293
http://dx.doi.org/10.4161/epi.28790
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