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A simple method based on laboratory inoculum and field inoculum for evaluating potato resistance to black scurf caused by Rhizoctonia solani

A two-step method was developed to evaluate potato resistance to black scurf caused by Rhizoctonia solani. Tuber piece inoculum was first conducted in the laboratory, which was also first reported in this study. After inoculation with pathogen discs and culture for 48 h, the necrotic spots on the in...

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Autores principales: Zhang, Xiao-Yu, Yu, Xiao-Xia, Yu, Zhuo, Xue, Yu-Feng, Qi, Li-Peng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Japanese Society of Breeding 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4065323/
https://www.ncbi.nlm.nih.gov/pubmed/24987302
http://dx.doi.org/10.1270/jsbbs.64.156
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author Zhang, Xiao-Yu
Yu, Xiao-Xia
Yu, Zhuo
Xue, Yu-Feng
Qi, Li-Peng
author_facet Zhang, Xiao-Yu
Yu, Xiao-Xia
Yu, Zhuo
Xue, Yu-Feng
Qi, Li-Peng
author_sort Zhang, Xiao-Yu
collection PubMed
description A two-step method was developed to evaluate potato resistance to black scurf caused by Rhizoctonia solani. Tuber piece inoculum was first conducted in the laboratory, which was also first reported in this study. After inoculation with pathogen discs and culture for 48 h, the necrotic spots on the inoculated potato pieces were generated and measured by the crossing method. Further evaluation was conducted through field experiments using a wheat bran inoculum method. The wheat bran inoculum was placed into the pit dispersedly and surrounded seed tubers. Each cultivar or line was subjected to five treatments of 0-, 2-, 3-, 4-, and 5-g soil inoculum. The results showed that 2–4 g of wheat bran inoculum was the optimum for identifying tuber black scurf resistance. The laboratory scores positively correlated with the incidence and severity of black scurf in the field. According to the results in the laboratory, relatively resistant cultivars could be selected for further estimation of tuber black scurf resistance in field experiments. It is a practical and effective screening method for rapid identification of resistant potato germplasm, which can reduce workload in the field, shorten time required for identification.
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spelling pubmed-40653232014-07-22 A simple method based on laboratory inoculum and field inoculum for evaluating potato resistance to black scurf caused by Rhizoctonia solani Zhang, Xiao-Yu Yu, Xiao-Xia Yu, Zhuo Xue, Yu-Feng Qi, Li-Peng Breed Sci Research Papers A two-step method was developed to evaluate potato resistance to black scurf caused by Rhizoctonia solani. Tuber piece inoculum was first conducted in the laboratory, which was also first reported in this study. After inoculation with pathogen discs and culture for 48 h, the necrotic spots on the inoculated potato pieces were generated and measured by the crossing method. Further evaluation was conducted through field experiments using a wheat bran inoculum method. The wheat bran inoculum was placed into the pit dispersedly and surrounded seed tubers. Each cultivar or line was subjected to five treatments of 0-, 2-, 3-, 4-, and 5-g soil inoculum. The results showed that 2–4 g of wheat bran inoculum was the optimum for identifying tuber black scurf resistance. The laboratory scores positively correlated with the incidence and severity of black scurf in the field. According to the results in the laboratory, relatively resistant cultivars could be selected for further estimation of tuber black scurf resistance in field experiments. It is a practical and effective screening method for rapid identification of resistant potato germplasm, which can reduce workload in the field, shorten time required for identification. Japanese Society of Breeding 2014-06 2014-06-01 /pmc/articles/PMC4065323/ /pubmed/24987302 http://dx.doi.org/10.1270/jsbbs.64.156 Text en Copyright © 2014 by JAPANESE SOCIETY OF BREEDING http://creativecommons.org/licenses/by-nc-nd/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Papers
Zhang, Xiao-Yu
Yu, Xiao-Xia
Yu, Zhuo
Xue, Yu-Feng
Qi, Li-Peng
A simple method based on laboratory inoculum and field inoculum for evaluating potato resistance to black scurf caused by Rhizoctonia solani
title A simple method based on laboratory inoculum and field inoculum for evaluating potato resistance to black scurf caused by Rhizoctonia solani
title_full A simple method based on laboratory inoculum and field inoculum for evaluating potato resistance to black scurf caused by Rhizoctonia solani
title_fullStr A simple method based on laboratory inoculum and field inoculum for evaluating potato resistance to black scurf caused by Rhizoctonia solani
title_full_unstemmed A simple method based on laboratory inoculum and field inoculum for evaluating potato resistance to black scurf caused by Rhizoctonia solani
title_short A simple method based on laboratory inoculum and field inoculum for evaluating potato resistance to black scurf caused by Rhizoctonia solani
title_sort simple method based on laboratory inoculum and field inoculum for evaluating potato resistance to black scurf caused by rhizoctonia solani
topic Research Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4065323/
https://www.ncbi.nlm.nih.gov/pubmed/24987302
http://dx.doi.org/10.1270/jsbbs.64.156
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