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Homogalacturonan deesterification during pollen–ovule interaction in Larix decidua Mill.: an immunocytochemical study

Studies on angiosperm plants have shown that homogalacturonan present in the extracellular matrix of pistils plays an important role in the interaction with the male gametophyte. However, in gymnosperms, knowledge on the participation of HG in the pollen–ovule interaction is limited, and only a few...

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Autores principales: Rafińska, Katarzyna, Świdziński, Michał, Bednarska-Kozakiewicz, Elżbieta
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4065381/
https://www.ncbi.nlm.nih.gov/pubmed/24793355
http://dx.doi.org/10.1007/s00425-014-2074-6
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author Rafińska, Katarzyna
Świdziński, Michał
Bednarska-Kozakiewicz, Elżbieta
author_facet Rafińska, Katarzyna
Świdziński, Michał
Bednarska-Kozakiewicz, Elżbieta
author_sort Rafińska, Katarzyna
collection PubMed
description Studies on angiosperm plants have shown that homogalacturonan present in the extracellular matrix of pistils plays an important role in the interaction with the male gametophyte. However, in gymnosperms, knowledge on the participation of HG in the pollen–ovule interaction is limited, and only a few studies on male gametophytes have been reported. Thus, the aim of this study was to determine the distribution of HG in male gametophytes and ovules during their interaction in Larix decidua Mill. The distribution of HG in pollen grains and unpollinated and pollinated ovules was investigated by immunofluorescence techniques using monoclonal antibodies that recognise high methyl-esterified HG (JIM7), low methyl-esterified HG (JIM5) and calcium cross-linked HG (2F4). All studied categories of HG were detected in the ovule. Highly methyl-esterified HG was present in the cell walls of all cells throughout the interaction; however, the distribution of low methyl-esterified and calcium cross-linked HG changed during the course of interaction. Both of these categories of HG appeared only in the apoplast and the extracellular matrix of the ovule tissues, which interact with the male gametophyte. This finding suggests that in L. decidua, low methyl-esterified and calcium cross-linked HG play an important role in pollen–ovule interaction. The last category of HG is most likely involved in adhesion between the pollen and the ovule and might provide an optimal calcium environment for pollen grain germination and pollen tube growth.
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spelling pubmed-40653812014-06-23 Homogalacturonan deesterification during pollen–ovule interaction in Larix decidua Mill.: an immunocytochemical study Rafińska, Katarzyna Świdziński, Michał Bednarska-Kozakiewicz, Elżbieta Planta Original Article Studies on angiosperm plants have shown that homogalacturonan present in the extracellular matrix of pistils plays an important role in the interaction with the male gametophyte. However, in gymnosperms, knowledge on the participation of HG in the pollen–ovule interaction is limited, and only a few studies on male gametophytes have been reported. Thus, the aim of this study was to determine the distribution of HG in male gametophytes and ovules during their interaction in Larix decidua Mill. The distribution of HG in pollen grains and unpollinated and pollinated ovules was investigated by immunofluorescence techniques using monoclonal antibodies that recognise high methyl-esterified HG (JIM7), low methyl-esterified HG (JIM5) and calcium cross-linked HG (2F4). All studied categories of HG were detected in the ovule. Highly methyl-esterified HG was present in the cell walls of all cells throughout the interaction; however, the distribution of low methyl-esterified and calcium cross-linked HG changed during the course of interaction. Both of these categories of HG appeared only in the apoplast and the extracellular matrix of the ovule tissues, which interact with the male gametophyte. This finding suggests that in L. decidua, low methyl-esterified and calcium cross-linked HG play an important role in pollen–ovule interaction. The last category of HG is most likely involved in adhesion between the pollen and the ovule and might provide an optimal calcium environment for pollen grain germination and pollen tube growth. Springer Berlin Heidelberg 2014-05-04 2014 /pmc/articles/PMC4065381/ /pubmed/24793355 http://dx.doi.org/10.1007/s00425-014-2074-6 Text en © The Author(s) 2014 https://creativecommons.org/licenses/by/4.0/ Open AccessThis article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.
spellingShingle Original Article
Rafińska, Katarzyna
Świdziński, Michał
Bednarska-Kozakiewicz, Elżbieta
Homogalacturonan deesterification during pollen–ovule interaction in Larix decidua Mill.: an immunocytochemical study
title Homogalacturonan deesterification during pollen–ovule interaction in Larix decidua Mill.: an immunocytochemical study
title_full Homogalacturonan deesterification during pollen–ovule interaction in Larix decidua Mill.: an immunocytochemical study
title_fullStr Homogalacturonan deesterification during pollen–ovule interaction in Larix decidua Mill.: an immunocytochemical study
title_full_unstemmed Homogalacturonan deesterification during pollen–ovule interaction in Larix decidua Mill.: an immunocytochemical study
title_short Homogalacturonan deesterification during pollen–ovule interaction in Larix decidua Mill.: an immunocytochemical study
title_sort homogalacturonan deesterification during pollen–ovule interaction in larix decidua mill.: an immunocytochemical study
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4065381/
https://www.ncbi.nlm.nih.gov/pubmed/24793355
http://dx.doi.org/10.1007/s00425-014-2074-6
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