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Nanofibrous Chitosan-Polyethylene Oxide Engineered Scaffolds: A Comparative Study between Simulated Structural Characteristics and Cells Viability

3D nanofibrous chitosan-polyethylene oxide (PEO) scaffolds were fabricated by electrospinning at different processing parameters. The structural characteristics, such as pore size, overall porosity, pore interconnectivity, and scaffold percolative efficiency (SPE), were simulated by a robust image a...

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Autores principales: Kazemi Pilehrood, Mohammad, Dilamian, Mandana, Mirian, Mina, Sadeghi-Aliabadi, Hojjat, Maleknia, Laleh, Nousiainen, Pertti, Harlin, Ali
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4065727/
https://www.ncbi.nlm.nih.gov/pubmed/24995296
http://dx.doi.org/10.1155/2014/438065
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author Kazemi Pilehrood, Mohammad
Dilamian, Mandana
Mirian, Mina
Sadeghi-Aliabadi, Hojjat
Maleknia, Laleh
Nousiainen, Pertti
Harlin, Ali
author_facet Kazemi Pilehrood, Mohammad
Dilamian, Mandana
Mirian, Mina
Sadeghi-Aliabadi, Hojjat
Maleknia, Laleh
Nousiainen, Pertti
Harlin, Ali
author_sort Kazemi Pilehrood, Mohammad
collection PubMed
description 3D nanofibrous chitosan-polyethylene oxide (PEO) scaffolds were fabricated by electrospinning at different processing parameters. The structural characteristics, such as pore size, overall porosity, pore interconnectivity, and scaffold percolative efficiency (SPE), were simulated by a robust image analysis. Mouse fibroblast cells (L929) were cultured in RPMI for 2 days in the presence of various samples of nanofibrous chitosan/PEO scaffolds. Cell attachments and corresponding mean viability were enhanced from 50% to 110% compared to that belonging to a control even at packed morphologies of scaffolds constituted from pores with nanoscale diameter. To elucidate the correlation between structural characteristics within the depth of the scaffolds' profile and cell viability, a comparative analysis was proposed. This analysis revealed that larger fiber diameters and pore sizes can enhance cell viability. On the contrary, increasing the other structural elements such as overall porosity and interconnectivity due to a simultaneous reduction in fiber diameter and pore size through the electrospinning process can reduce the viability of cells. In addition, it was found that manipulation of the processing parameters in electrospinning can compensate for the effects of packed morphologies of nanofibrous scaffolds and can thus potentially improve the infiltration and viability of cells.
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spelling pubmed-40657272014-07-03 Nanofibrous Chitosan-Polyethylene Oxide Engineered Scaffolds: A Comparative Study between Simulated Structural Characteristics and Cells Viability Kazemi Pilehrood, Mohammad Dilamian, Mandana Mirian, Mina Sadeghi-Aliabadi, Hojjat Maleknia, Laleh Nousiainen, Pertti Harlin, Ali Biomed Res Int Research Article 3D nanofibrous chitosan-polyethylene oxide (PEO) scaffolds were fabricated by electrospinning at different processing parameters. The structural characteristics, such as pore size, overall porosity, pore interconnectivity, and scaffold percolative efficiency (SPE), were simulated by a robust image analysis. Mouse fibroblast cells (L929) were cultured in RPMI for 2 days in the presence of various samples of nanofibrous chitosan/PEO scaffolds. Cell attachments and corresponding mean viability were enhanced from 50% to 110% compared to that belonging to a control even at packed morphologies of scaffolds constituted from pores with nanoscale diameter. To elucidate the correlation between structural characteristics within the depth of the scaffolds' profile and cell viability, a comparative analysis was proposed. This analysis revealed that larger fiber diameters and pore sizes can enhance cell viability. On the contrary, increasing the other structural elements such as overall porosity and interconnectivity due to a simultaneous reduction in fiber diameter and pore size through the electrospinning process can reduce the viability of cells. In addition, it was found that manipulation of the processing parameters in electrospinning can compensate for the effects of packed morphologies of nanofibrous scaffolds and can thus potentially improve the infiltration and viability of cells. Hindawi Publishing Corporation 2014 2014-06-04 /pmc/articles/PMC4065727/ /pubmed/24995296 http://dx.doi.org/10.1155/2014/438065 Text en Copyright © 2014 Mohammad Kazemi Pilehrood et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Kazemi Pilehrood, Mohammad
Dilamian, Mandana
Mirian, Mina
Sadeghi-Aliabadi, Hojjat
Maleknia, Laleh
Nousiainen, Pertti
Harlin, Ali
Nanofibrous Chitosan-Polyethylene Oxide Engineered Scaffolds: A Comparative Study between Simulated Structural Characteristics and Cells Viability
title Nanofibrous Chitosan-Polyethylene Oxide Engineered Scaffolds: A Comparative Study between Simulated Structural Characteristics and Cells Viability
title_full Nanofibrous Chitosan-Polyethylene Oxide Engineered Scaffolds: A Comparative Study between Simulated Structural Characteristics and Cells Viability
title_fullStr Nanofibrous Chitosan-Polyethylene Oxide Engineered Scaffolds: A Comparative Study between Simulated Structural Characteristics and Cells Viability
title_full_unstemmed Nanofibrous Chitosan-Polyethylene Oxide Engineered Scaffolds: A Comparative Study between Simulated Structural Characteristics and Cells Viability
title_short Nanofibrous Chitosan-Polyethylene Oxide Engineered Scaffolds: A Comparative Study between Simulated Structural Characteristics and Cells Viability
title_sort nanofibrous chitosan-polyethylene oxide engineered scaffolds: a comparative study between simulated structural characteristics and cells viability
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4065727/
https://www.ncbi.nlm.nih.gov/pubmed/24995296
http://dx.doi.org/10.1155/2014/438065
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