Convenient, Sensitive and High-Throughput Method for Screening Botanic Origin

In this work, a rapid (within 4–5 h), sensitive and visible new method for assessing botanic origin is developed by combining loop-mediated isothermal amplification with cationic conjugated polymers. The two Chinese medicinal materials (Jin-Yin-Hua and Shan-Yin-Hua) with similar morphology and chemi...

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Detalles Bibliográficos
Autores principales: Yuan, Yuan, Jiang, Chao, Liu, Libing, Yu, Shulin, Cui, Zhanhu, Chen, Min, Lin, Shufang, Wang, Shu, Huang, Luqi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4066250/
https://www.ncbi.nlm.nih.gov/pubmed/24953704
http://dx.doi.org/10.1038/srep05395
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author Yuan, Yuan
Jiang, Chao
Liu, Libing
Yu, Shulin
Cui, Zhanhu
Chen, Min
Lin, Shufang
Wang, Shu
Huang, Luqi
author_facet Yuan, Yuan
Jiang, Chao
Liu, Libing
Yu, Shulin
Cui, Zhanhu
Chen, Min
Lin, Shufang
Wang, Shu
Huang, Luqi
author_sort Yuan, Yuan
collection PubMed
description In this work, a rapid (within 4–5 h), sensitive and visible new method for assessing botanic origin is developed by combining loop-mediated isothermal amplification with cationic conjugated polymers. The two Chinese medicinal materials (Jin-Yin-Hua and Shan-Yin-Hua) with similar morphology and chemical composition were clearly distinguished by gene SNP genotyping assays. The identification of plant species in Patented Chinese drugs containing Lonicera buds is successfully performed using this detection system. The method is also robust enough to be used in high-throughput screening. This new method is very helpful to identify herbal materials, and is beneficial for detecting safety and quality of botanic products.
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spelling pubmed-40662502014-06-23 Convenient, Sensitive and High-Throughput Method for Screening Botanic Origin Yuan, Yuan Jiang, Chao Liu, Libing Yu, Shulin Cui, Zhanhu Chen, Min Lin, Shufang Wang, Shu Huang, Luqi Sci Rep Article In this work, a rapid (within 4–5 h), sensitive and visible new method for assessing botanic origin is developed by combining loop-mediated isothermal amplification with cationic conjugated polymers. The two Chinese medicinal materials (Jin-Yin-Hua and Shan-Yin-Hua) with similar morphology and chemical composition were clearly distinguished by gene SNP genotyping assays. The identification of plant species in Patented Chinese drugs containing Lonicera buds is successfully performed using this detection system. The method is also robust enough to be used in high-throughput screening. This new method is very helpful to identify herbal materials, and is beneficial for detecting safety and quality of botanic products. Nature Publishing Group 2014-06-23 /pmc/articles/PMC4066250/ /pubmed/24953704 http://dx.doi.org/10.1038/srep05395 Text en Copyright © 2014, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by-nc-sa/4.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/4.0/
spellingShingle Article
Yuan, Yuan
Jiang, Chao
Liu, Libing
Yu, Shulin
Cui, Zhanhu
Chen, Min
Lin, Shufang
Wang, Shu
Huang, Luqi
Convenient, Sensitive and High-Throughput Method for Screening Botanic Origin
title Convenient, Sensitive and High-Throughput Method for Screening Botanic Origin
title_full Convenient, Sensitive and High-Throughput Method for Screening Botanic Origin
title_fullStr Convenient, Sensitive and High-Throughput Method for Screening Botanic Origin
title_full_unstemmed Convenient, Sensitive and High-Throughput Method for Screening Botanic Origin
title_short Convenient, Sensitive and High-Throughput Method for Screening Botanic Origin
title_sort convenient, sensitive and high-throughput method for screening botanic origin
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4066250/
https://www.ncbi.nlm.nih.gov/pubmed/24953704
http://dx.doi.org/10.1038/srep05395
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