Multiplex genome engineering in human cells using all-in-one CRISPR/Cas9 vector system

CRISPR/Cas9-mediated genome editing is a next-generation strategy for genetic modifications, not only for single gene targeting, but also for multiple targeted mutagenesis. To make the most of the multiplexity of CRISPR/Cas9, we established a system for constructing all-in-one expression vectors con...

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Detalles Bibliográficos
Autores principales: Sakuma, Tetsushi, Nishikawa, Ayami, Kume, Satoshi, Chayama, Kazuaki, Yamamoto, Takashi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4066266/
https://www.ncbi.nlm.nih.gov/pubmed/24954249
http://dx.doi.org/10.1038/srep05400

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4066266/
https://www.ncbi.nlm.nih.gov/pubmed/24954249
http://dx.doi.org/10.1038/srep05400

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