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Effect of Androsterone after Pilocarpine-induced Status Epilepticus in Mice

BACKGROUND AND PURPOSE: Neurosteroids exert their antiepileptic effects via GABAA and NMDA receptors. Another cell death mechanism is excessive Ca(2+) influx into cells. Calbindin-D28k (CB) is a protein that modulates intracellular Ca(2+) in the nervous system. We evaluated whether androsterone up-r...

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Autores principales: Cho, Inja, Cho, Yang-Je, Kim, Hyun-Woo, Heo, Kyung, Lee, Byung-In, Kim, Won-Joo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korean Epilepsy Society 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4066622/
https://www.ncbi.nlm.nih.gov/pubmed/24977124
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author Cho, Inja
Cho, Yang-Je
Kim, Hyun-Woo
Heo, Kyung
Lee, Byung-In
Kim, Won-Joo
author_facet Cho, Inja
Cho, Yang-Je
Kim, Hyun-Woo
Heo, Kyung
Lee, Byung-In
Kim, Won-Joo
author_sort Cho, Inja
collection PubMed
description BACKGROUND AND PURPOSE: Neurosteroids exert their antiepileptic effects via GABAA and NMDA receptors. Another cell death mechanism is excessive Ca(2+) influx into cells. Calbindin-D28k (CB) is a protein that modulates intracellular Ca(2+) in the nervous system. We evaluated whether androsterone up-regulates the expression of CB and has a neuroprotective effect by controlling Ca(2+) after pilocarpine-induced status epilepticus (SE) in mice. METHODS: SE was induced in ICR mice by injection of pilocarpine. Two hours after SE, mice were treated intraperitoneally (i.p.) with androsterone (100–200 mg/kg) or vehicle, and compared with other control groups. Two days after injection, immunohistochemical staining for CB was performed using a hippocampal slice from each mice group. We also used cresyl violet staining to compare changes in hippocampal structures. RESULTS: Two days after pilocarpine-induced SE, androsterone increased the expression of CB in the hippocampus compared with control SE mice. The number of CB-positive cells was 1±0.4 cells/mm(3) in pilocarpine-only group, 14±1.1 cells/mm(3) in pilocarpine plus androsterone 100 mg group and 29±2.5 cells/mm(3) in pilocarpine plus androsterone 200 mg group (p<0.001). CONCLUSIONS: These results suggest that the neuroprotective effect of androsterone after pilocarpine- induced SE may be mediated by an increased expression of CB.
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spelling pubmed-40666222014-06-30 Effect of Androsterone after Pilocarpine-induced Status Epilepticus in Mice Cho, Inja Cho, Yang-Je Kim, Hyun-Woo Heo, Kyung Lee, Byung-In Kim, Won-Joo J Epilepsy Res Original Article BACKGROUND AND PURPOSE: Neurosteroids exert their antiepileptic effects via GABAA and NMDA receptors. Another cell death mechanism is excessive Ca(2+) influx into cells. Calbindin-D28k (CB) is a protein that modulates intracellular Ca(2+) in the nervous system. We evaluated whether androsterone up-regulates the expression of CB and has a neuroprotective effect by controlling Ca(2+) after pilocarpine-induced status epilepticus (SE) in mice. METHODS: SE was induced in ICR mice by injection of pilocarpine. Two hours after SE, mice were treated intraperitoneally (i.p.) with androsterone (100–200 mg/kg) or vehicle, and compared with other control groups. Two days after injection, immunohistochemical staining for CB was performed using a hippocampal slice from each mice group. We also used cresyl violet staining to compare changes in hippocampal structures. RESULTS: Two days after pilocarpine-induced SE, androsterone increased the expression of CB in the hippocampus compared with control SE mice. The number of CB-positive cells was 1±0.4 cells/mm(3) in pilocarpine-only group, 14±1.1 cells/mm(3) in pilocarpine plus androsterone 100 mg group and 29±2.5 cells/mm(3) in pilocarpine plus androsterone 200 mg group (p<0.001). CONCLUSIONS: These results suggest that the neuroprotective effect of androsterone after pilocarpine- induced SE may be mediated by an increased expression of CB. Korean Epilepsy Society 2014-06-30 /pmc/articles/PMC4066622/ /pubmed/24977124 Text en Copyright©2014 Korean Epilepsy Society This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Cho, Inja
Cho, Yang-Je
Kim, Hyun-Woo
Heo, Kyung
Lee, Byung-In
Kim, Won-Joo
Effect of Androsterone after Pilocarpine-induced Status Epilepticus in Mice
title Effect of Androsterone after Pilocarpine-induced Status Epilepticus in Mice
title_full Effect of Androsterone after Pilocarpine-induced Status Epilepticus in Mice
title_fullStr Effect of Androsterone after Pilocarpine-induced Status Epilepticus in Mice
title_full_unstemmed Effect of Androsterone after Pilocarpine-induced Status Epilepticus in Mice
title_short Effect of Androsterone after Pilocarpine-induced Status Epilepticus in Mice
title_sort effect of androsterone after pilocarpine-induced status epilepticus in mice
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4066622/
https://www.ncbi.nlm.nih.gov/pubmed/24977124
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