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A complete landscape of post-transcriptional modifications in mammalian mitochondrial tRNAs
In mammalian mitochondria, 22 species of tRNAs encoded in mitochondrial DNA play crucial roles in the translation of 13 essential subunits of the respiratory chain complexes involved in oxidative phosphorylation. Following transcription, mitochondrial tRNAs are modified by nuclear-encoded tRNA-modif...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4066797/ https://www.ncbi.nlm.nih.gov/pubmed/24831542 http://dx.doi.org/10.1093/nar/gku390 |
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author | Suzuki, Takeo Suzuki, Tsutomu |
author_facet | Suzuki, Takeo Suzuki, Tsutomu |
author_sort | Suzuki, Takeo |
collection | PubMed |
description | In mammalian mitochondria, 22 species of tRNAs encoded in mitochondrial DNA play crucial roles in the translation of 13 essential subunits of the respiratory chain complexes involved in oxidative phosphorylation. Following transcription, mitochondrial tRNAs are modified by nuclear-encoded tRNA-modifying enzymes. These modifications are required for the proper functioning of mitochondrial tRNAs (mt tRNAs), and the absence of these modifications can cause pathological consequences. To date, however, the information available about these modifications has been incomplete. To address this issue, we isolated all 22 species of mt tRNAs from bovine liver and comprehensively determined the post-transcriptional modifications in each tRNA by mass spectrometry. Here, we describe the primary structures with post-transcriptional modifications of seven species of mt tRNAs which were previously uncharacterized, and provide revised information regarding base modifications in five other mt tRNAs. In the complete set of bovine mt tRNAs, we found 15 species of modified nucleosides at 118 positions (7.48% of total bases). This result provides insight into the molecular mechanisms underlying the decoding system in mammalian mitochondria and enables prediction of candidate tRNA-modifying enzymes responsible for each modification of mt tRNAs. |
format | Online Article Text |
id | pubmed-4066797 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-40667972014-06-24 A complete landscape of post-transcriptional modifications in mammalian mitochondrial tRNAs Suzuki, Takeo Suzuki, Tsutomu Nucleic Acids Res RNA In mammalian mitochondria, 22 species of tRNAs encoded in mitochondrial DNA play crucial roles in the translation of 13 essential subunits of the respiratory chain complexes involved in oxidative phosphorylation. Following transcription, mitochondrial tRNAs are modified by nuclear-encoded tRNA-modifying enzymes. These modifications are required for the proper functioning of mitochondrial tRNAs (mt tRNAs), and the absence of these modifications can cause pathological consequences. To date, however, the information available about these modifications has been incomplete. To address this issue, we isolated all 22 species of mt tRNAs from bovine liver and comprehensively determined the post-transcriptional modifications in each tRNA by mass spectrometry. Here, we describe the primary structures with post-transcriptional modifications of seven species of mt tRNAs which were previously uncharacterized, and provide revised information regarding base modifications in five other mt tRNAs. In the complete set of bovine mt tRNAs, we found 15 species of modified nucleosides at 118 positions (7.48% of total bases). This result provides insight into the molecular mechanisms underlying the decoding system in mammalian mitochondria and enables prediction of candidate tRNA-modifying enzymes responsible for each modification of mt tRNAs. Oxford University Press 2014-07-01 2014-05-15 /pmc/articles/PMC4066797/ /pubmed/24831542 http://dx.doi.org/10.1093/nar/gku390 Text en © The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research. http://creativecommons.org/licenses/by/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | RNA Suzuki, Takeo Suzuki, Tsutomu A complete landscape of post-transcriptional modifications in mammalian mitochondrial tRNAs |
title | A complete landscape of post-transcriptional modifications in mammalian mitochondrial tRNAs |
title_full | A complete landscape of post-transcriptional modifications in mammalian mitochondrial tRNAs |
title_fullStr | A complete landscape of post-transcriptional modifications in mammalian mitochondrial tRNAs |
title_full_unstemmed | A complete landscape of post-transcriptional modifications in mammalian mitochondrial tRNAs |
title_short | A complete landscape of post-transcriptional modifications in mammalian mitochondrial tRNAs |
title_sort | complete landscape of post-transcriptional modifications in mammalian mitochondrial trnas |
topic | RNA |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4066797/ https://www.ncbi.nlm.nih.gov/pubmed/24831542 http://dx.doi.org/10.1093/nar/gku390 |
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