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A complete landscape of post-transcriptional modifications in mammalian mitochondrial tRNAs

In mammalian mitochondria, 22 species of tRNAs encoded in mitochondrial DNA play crucial roles in the translation of 13 essential subunits of the respiratory chain complexes involved in oxidative phosphorylation. Following transcription, mitochondrial tRNAs are modified by nuclear-encoded tRNA-modif...

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Detalles Bibliográficos
Autores principales: Suzuki, Takeo, Suzuki, Tsutomu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2014
Materias:
RNA
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4066797/
https://www.ncbi.nlm.nih.gov/pubmed/24831542
http://dx.doi.org/10.1093/nar/gku390
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author Suzuki, Takeo
Suzuki, Tsutomu
author_facet Suzuki, Takeo
Suzuki, Tsutomu
author_sort Suzuki, Takeo
collection PubMed
description In mammalian mitochondria, 22 species of tRNAs encoded in mitochondrial DNA play crucial roles in the translation of 13 essential subunits of the respiratory chain complexes involved in oxidative phosphorylation. Following transcription, mitochondrial tRNAs are modified by nuclear-encoded tRNA-modifying enzymes. These modifications are required for the proper functioning of mitochondrial tRNAs (mt tRNAs), and the absence of these modifications can cause pathological consequences. To date, however, the information available about these modifications has been incomplete. To address this issue, we isolated all 22 species of mt tRNAs from bovine liver and comprehensively determined the post-transcriptional modifications in each tRNA by mass spectrometry. Here, we describe the primary structures with post-transcriptional modifications of seven species of mt tRNAs which were previously uncharacterized, and provide revised information regarding base modifications in five other mt tRNAs. In the complete set of bovine mt tRNAs, we found 15 species of modified nucleosides at 118 positions (7.48% of total bases). This result provides insight into the molecular mechanisms underlying the decoding system in mammalian mitochondria and enables prediction of candidate tRNA-modifying enzymes responsible for each modification of mt tRNAs.
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spelling pubmed-40667972014-06-24 A complete landscape of post-transcriptional modifications in mammalian mitochondrial tRNAs Suzuki, Takeo Suzuki, Tsutomu Nucleic Acids Res RNA In mammalian mitochondria, 22 species of tRNAs encoded in mitochondrial DNA play crucial roles in the translation of 13 essential subunits of the respiratory chain complexes involved in oxidative phosphorylation. Following transcription, mitochondrial tRNAs are modified by nuclear-encoded tRNA-modifying enzymes. These modifications are required for the proper functioning of mitochondrial tRNAs (mt tRNAs), and the absence of these modifications can cause pathological consequences. To date, however, the information available about these modifications has been incomplete. To address this issue, we isolated all 22 species of mt tRNAs from bovine liver and comprehensively determined the post-transcriptional modifications in each tRNA by mass spectrometry. Here, we describe the primary structures with post-transcriptional modifications of seven species of mt tRNAs which were previously uncharacterized, and provide revised information regarding base modifications in five other mt tRNAs. In the complete set of bovine mt tRNAs, we found 15 species of modified nucleosides at 118 positions (7.48% of total bases). This result provides insight into the molecular mechanisms underlying the decoding system in mammalian mitochondria and enables prediction of candidate tRNA-modifying enzymes responsible for each modification of mt tRNAs. Oxford University Press 2014-07-01 2014-05-15 /pmc/articles/PMC4066797/ /pubmed/24831542 http://dx.doi.org/10.1093/nar/gku390 Text en © The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research. http://creativecommons.org/licenses/by/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle RNA
Suzuki, Takeo
Suzuki, Tsutomu
A complete landscape of post-transcriptional modifications in mammalian mitochondrial tRNAs
title A complete landscape of post-transcriptional modifications in mammalian mitochondrial tRNAs
title_full A complete landscape of post-transcriptional modifications in mammalian mitochondrial tRNAs
title_fullStr A complete landscape of post-transcriptional modifications in mammalian mitochondrial tRNAs
title_full_unstemmed A complete landscape of post-transcriptional modifications in mammalian mitochondrial tRNAs
title_short A complete landscape of post-transcriptional modifications in mammalian mitochondrial tRNAs
title_sort complete landscape of post-transcriptional modifications in mammalian mitochondrial trnas
topic RNA
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4066797/
https://www.ncbi.nlm.nih.gov/pubmed/24831542
http://dx.doi.org/10.1093/nar/gku390
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